Myotis polyomavirus VM-2008

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus; Myotis lucifugus polyomavirus 1

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6E000|B6E000_9POLY Minor capsid protein OS=Myotis polyomavirus VM-2008 OX=563775 PE=3 SV=1
MM1 pKa = 7.38GALLTVLAEE10 pKa = 4.4VIEE13 pKa = 4.3LASVTGLSAEE23 pKa = 4.43SIISGEE29 pKa = 4.03AFATAEE35 pKa = 4.25LLEE38 pKa = 4.17SHH40 pKa = 7.11IANLVTYY47 pKa = 10.3GGLTEE52 pKa = 4.96AEE54 pKa = 3.92ALAATEE60 pKa = 4.16VTTEE64 pKa = 4.03AFEE67 pKa = 4.51ALQSLTPNFSQVFGAIAGIDD87 pKa = 3.15IAANSSLFLGAAVAAALYY105 pKa = 8.64PYY107 pKa = 10.13NWDD110 pKa = 3.32YY111 pKa = 11.26SQPIAMANMDD121 pKa = 4.14YY122 pKa = 10.56PIVLYY127 pKa = 10.78HH128 pKa = 7.39PDD130 pKa = 3.63FDD132 pKa = 4.65IDD134 pKa = 4.24FPGLRR139 pKa = 11.84TLARR143 pKa = 11.84FIHH146 pKa = 6.33YY147 pKa = 9.88ISPEE151 pKa = 3.49HH152 pKa = 6.44WGADD156 pKa = 3.4LFRR159 pKa = 11.84AMGNYY164 pKa = 9.99LYY166 pKa = 10.8EE167 pKa = 4.09LLQRR171 pKa = 11.84EE172 pKa = 4.43RR173 pKa = 11.84RR174 pKa = 11.84RR175 pKa = 11.84QIDD178 pKa = 3.07RR179 pKa = 11.84MTRR182 pKa = 11.84EE183 pKa = 3.63LARR186 pKa = 11.84RR187 pKa = 11.84SGEE190 pKa = 3.98EE191 pKa = 3.64VADD194 pKa = 3.98RR195 pKa = 11.84LARR198 pKa = 11.84YY199 pKa = 9.22FEE201 pKa = 3.92NARR204 pKa = 11.84WAVTNAISTPVTIYY218 pKa = 10.5RR219 pKa = 11.84YY220 pKa = 10.37LGDD223 pKa = 3.85YY224 pKa = 10.18YY225 pKa = 10.92RR226 pKa = 11.84ALPGPNPIRR235 pKa = 11.84ARR237 pKa = 11.84QLARR241 pKa = 11.84SLGQEE246 pKa = 3.46EE247 pKa = 5.4PYY249 pKa = 10.39RR250 pKa = 11.84YY251 pKa = 10.31DD252 pKa = 3.46RR253 pKa = 11.84FEE255 pKa = 4.33TEE257 pKa = 3.45HH258 pKa = 6.84GEE260 pKa = 3.95PSFTPKK266 pKa = 10.06QKK268 pKa = 10.36SADD271 pKa = 3.5YY272 pKa = 10.32VEE274 pKa = 5.85RR275 pKa = 11.84YY276 pKa = 7.48EE277 pKa = 4.4PPGGAYY283 pKa = 9.39QRR285 pKa = 11.84NTPDD289 pKa = 2.53WMLPLILGLYY299 pKa = 10.37GDD301 pKa = 5.24ISPSWGDD308 pKa = 3.14TIEE311 pKa = 4.1RR312 pKa = 11.84VEE314 pKa = 4.13EE315 pKa = 4.13EE316 pKa = 4.32EE317 pKa = 5.02KK318 pKa = 11.11EE319 pKa = 4.24EE320 pKa = 4.15EE321 pKa = 4.85DD322 pKa = 4.14GPKK325 pKa = 9.92KK326 pKa = 10.44KK327 pKa = 10.28KK328 pKa = 10.2LRR330 pKa = 11.84TVRR333 pKa = 11.84GPKK336 pKa = 8.95TNNKK340 pKa = 8.73RR341 pKa = 11.84RR342 pKa = 11.84NRR344 pKa = 11.84STQRR348 pKa = 11.84PNRR351 pKa = 11.84PRR353 pKa = 4.21

Molecular weight: 39.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6DZZ9|B6DZZ9_9POLY Minor capsid protein OS=Myotis polyomavirus VM-2008 OX=563775 PE=3 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84GRR4 pKa = 11.84SSWYY8 pKa = 10.04RR9 pKa = 11.84RR10 pKa = 11.84WKK12 pKa = 10.42DD13 pKa = 3.15LLKK16 pKa = 10.67IFVNLNKK23 pKa = 10.21RR24 pKa = 11.84SGAPPRR30 pKa = 3.92

Molecular weight: 3.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1809

30

670

301.5

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.91 ± 1.051

1.879 ± 0.742

5.694 ± 0.562

7.463 ± 0.624

4.643 ± 0.555

5.804 ± 0.623

1.658 ± 0.191

5.086 ± 0.108

5.915 ± 1.028

9.121 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.208

4.422 ± 0.232

6.025 ± 0.73

4.367 ± 0.564

7.186 ± 1.328

5.694 ± 0.33

5.196 ± 0.232

4.865 ± 0.761

1.382 ± 0.253

4.256 ± 0.613
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski