Eubacteriaceae bacterium CHKCI004

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; unclassified Eubacteriaceae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3682 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143XJ95|A0A143XJ95_9FIRM Putative acetyltransferase OS=Eubacteriaceae bacterium CHKCI004 OX=1780380 GN=BN3660_00497 PE=4 SV=1
MM1 pKa = 8.15SEE3 pKa = 3.86FDD5 pKa = 3.95YY6 pKa = 11.66NNTNDD11 pKa = 4.65GLNNDD16 pKa = 4.05NTDD19 pKa = 3.31DD20 pKa = 4.57SLNHH24 pKa = 6.94DD25 pKa = 3.78PAGNDD30 pKa = 3.89SLNDD34 pKa = 3.47DD35 pKa = 4.71AGWNKK40 pKa = 10.42PGDD43 pKa = 4.93DD44 pKa = 3.73IDD46 pKa = 4.01TAASEE51 pKa = 4.4EE52 pKa = 4.57TGTSSYY58 pKa = 10.91EE59 pKa = 3.46PAAADD64 pKa = 3.47TAPADD69 pKa = 3.67GTYY72 pKa = 10.11HH73 pKa = 6.17YY74 pKa = 11.16VGDD77 pKa = 4.22PYY79 pKa = 10.97RR80 pKa = 11.84EE81 pKa = 3.98EE82 pKa = 4.53SGTASEE88 pKa = 4.9DD89 pKa = 3.25GRR91 pKa = 11.84NPRR94 pKa = 11.84YY95 pKa = 9.76RR96 pKa = 11.84YY97 pKa = 9.62QGQQNAQYY105 pKa = 10.38NNSFSEE111 pKa = 4.63GSWQGDD117 pKa = 3.54GTSADD122 pKa = 3.8SQQDD126 pKa = 3.16AQYY129 pKa = 11.51NGGAYY134 pKa = 10.08NEE136 pKa = 4.73SSQQYY141 pKa = 8.65YY142 pKa = 10.96SSIPDD147 pKa = 3.46EE148 pKa = 4.39PDD150 pKa = 2.78KK151 pKa = 11.4KK152 pKa = 10.41RR153 pKa = 11.84GGMGMRR159 pKa = 11.84IAKK162 pKa = 9.65IAGAALLFGIVAGVGFAGVSIARR185 pKa = 11.84DD186 pKa = 3.45RR187 pKa = 11.84LIPSATEE194 pKa = 3.72RR195 pKa = 11.84VQTTTVQNNGSDD207 pKa = 3.98DD208 pKa = 4.0SSSSSASAQDD218 pKa = 3.0ISNVVDD224 pKa = 3.51AVMPSVVSITSTTQVSNYY242 pKa = 9.48FFGTQEE248 pKa = 4.05SEE250 pKa = 4.26GAGSGFILAKK260 pKa = 10.07TDD262 pKa = 3.73DD263 pKa = 4.31ALMIATNNHH272 pKa = 4.31VVEE275 pKa = 4.58GANALTVGFSDD286 pKa = 4.06GTTADD291 pKa = 3.39ATIVGTDD298 pKa = 3.12SDD300 pKa = 4.27ADD302 pKa = 3.85LAVISVQASGLSEE315 pKa = 4.22DD316 pKa = 3.78TLSTIKK322 pKa = 10.75VAVLGSSDD330 pKa = 3.64DD331 pKa = 3.93LKK333 pKa = 11.42VGEE336 pKa = 4.26TVIAIGNALGYY347 pKa = 8.84GQSVTTGVVSAKK359 pKa = 10.24DD360 pKa = 3.57RR361 pKa = 11.84EE362 pKa = 4.33VSFTDD367 pKa = 3.38GTMTLLQTDD376 pKa = 3.91AAINPGNSGGVLVNLNGEE394 pKa = 4.33VVGINNAKK402 pKa = 10.68LEE404 pKa = 4.23DD405 pKa = 3.75TSVEE409 pKa = 4.0GMGYY413 pKa = 10.35AIPISTAQGTLEE425 pKa = 3.86TLMNSGSIPEE435 pKa = 4.4GEE437 pKa = 3.8AAYY440 pKa = 10.57LGILGRR446 pKa = 11.84TIDD449 pKa = 3.64STYY452 pKa = 11.08SEE454 pKa = 4.51ALGMPSGVYY463 pKa = 9.41VSQVVEE469 pKa = 4.11GSPAEE474 pKa = 4.13TAGIAAGDD482 pKa = 4.04VITGFEE488 pKa = 4.53GNTVSTMDD496 pKa = 3.67GLKK499 pKa = 10.69DD500 pKa = 4.18RR501 pKa = 11.84ISAQQAGTEE510 pKa = 4.16VQITLQRR517 pKa = 11.84ANQNGTYY524 pKa = 9.95EE525 pKa = 4.26EE526 pKa = 4.16QTVTVTLGKK535 pKa = 10.63EE536 pKa = 3.7SDD538 pKa = 3.91YY539 pKa = 11.54QDD541 pKa = 4.47AEE543 pKa = 4.37TATEE547 pKa = 4.1EE548 pKa = 4.49SADD551 pKa = 3.67TTEE554 pKa = 5.22QDD556 pKa = 3.31SQQYY560 pKa = 7.85GTDD563 pKa = 3.11PYY565 pKa = 11.11EE566 pKa = 4.23YY567 pKa = 10.84YY568 pKa = 10.91NGGNGNDD575 pKa = 4.3YY576 pKa = 10.96YY577 pKa = 11.3SSPYY581 pKa = 10.74DD582 pKa = 3.37YY583 pKa = 11.02FFNN586 pKa = 4.47

Molecular weight: 61.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143XYV9|A0A143XYV9_9FIRM VWA domain containing CoxE-like protein OS=Eubacteriaceae bacterium CHKCI004 OX=1780380 GN=BN3660_02929 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.03KK3 pKa = 10.06QRR5 pKa = 11.84VLLGRR10 pKa = 11.84TLAPSAVALATKK22 pKa = 7.91FVCARR27 pKa = 11.84VRR29 pKa = 11.84IPPGEE34 pKa = 3.88LLYY37 pKa = 10.89YY38 pKa = 9.99RR39 pKa = 11.84NSSGISYY46 pKa = 9.88NIKK49 pKa = 10.24KK50 pKa = 10.01LRR52 pKa = 11.84PCKK55 pKa = 9.51RR56 pKa = 11.84DD57 pKa = 3.22EE58 pKa = 4.42VIRR61 pKa = 11.84GSTLIDD67 pKa = 3.57AQTGHH72 pKa = 7.82PLTAVNAWYY81 pKa = 9.66VQRR84 pKa = 11.84SLSMPAGPSHH94 pKa = 6.5CQLPNALSRR103 pKa = 11.84FRR105 pKa = 11.84WNKK108 pKa = 9.55DD109 pKa = 2.73AFSLRR114 pKa = 11.84HH115 pKa = 5.8PLSVSLQNGTLFVLCLFHH133 pKa = 7.84IHH135 pKa = 7.31LSLCNFRR142 pKa = 11.84KK143 pKa = 9.35FVNCFPQKK151 pKa = 9.37MFSVQTISS159 pKa = 2.97

Molecular weight: 17.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3682

0

3682

1165227

29

5559

316.5

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.492 ± 0.042

1.483 ± 0.021

5.705 ± 0.038

7.912 ± 0.048

4.033 ± 0.029

7.023 ± 0.043

1.786 ± 0.017

7.057 ± 0.044

6.7 ± 0.042

8.835 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.086 ± 0.026

4.19 ± 0.03

3.333 ± 0.023

3.409 ± 0.023

4.957 ± 0.043

5.871 ± 0.034

5.373 ± 0.053

6.686 ± 0.036

0.909 ± 0.014

4.16 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski