Staphylococcus phage phiSa2wa_st93

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus; unclassified Triavirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6PEU0|A0A2I6PEU0_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSa2wa_st93 OX=2060956 PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 4.48MMNNRR7 pKa = 11.84EE8 pKa = 4.11QIEE11 pKa = 3.87QSIISASAYY20 pKa = 10.06NGNDD24 pKa = 3.29TEE26 pKa = 4.52GLLKK30 pKa = 10.49EE31 pKa = 4.44IEE33 pKa = 4.24DD34 pKa = 4.28VYY36 pKa = 11.57KK37 pKa = 10.64KK38 pKa = 10.72AQAFDD43 pKa = 4.66EE44 pKa = 4.34ILEE47 pKa = 4.2GLPNAMQDD55 pKa = 3.61ALKK58 pKa = 10.63EE59 pKa = 4.3DD60 pKa = 3.43IGLDD64 pKa = 3.48EE65 pKa = 5.32AVGIMTGQVVYY76 pKa = 10.26KK77 pKa = 10.68YY78 pKa = 10.96EE79 pKa = 4.05EE80 pKa = 4.33DD81 pKa = 3.75QEE83 pKa = 4.57NDD85 pKa = 2.92

Molecular weight: 9.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6PES5|A0A2I6PES5_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSa2wa_st93 OX=2060956 PE=4 SV=1
MM1 pKa = 7.63LFNFSLAPDD10 pKa = 4.35FSKK13 pKa = 11.23LLFNGLLRR21 pKa = 11.84VVSCFLFLSTIKK33 pKa = 10.57FSPPFNVYY41 pKa = 7.3TSRR44 pKa = 11.84RR45 pKa = 11.84FLIFFNPFSRR55 pKa = 11.84HH56 pKa = 5.68AII58 pKa = 3.72

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14638

34

1996

218.5

25.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.697 ± 0.598

0.547 ± 0.101

6.128 ± 0.254

7.59 ± 0.467

3.983 ± 0.218

6.012 ± 0.518

1.763 ± 0.136

7.064 ± 0.27

9.769 ± 0.47

8.184 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.698 ± 0.158

6.538 ± 0.31

2.726 ± 0.205

3.751 ± 0.168

4.119 ± 0.21

6.408 ± 0.366

5.725 ± 0.239

5.636 ± 0.2

1.264 ± 0.117

4.4 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski