Clostridium sp. Maddingley MBC34-26

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K6TT97|K6TT97_9CLOT CTP:phosphocholine cytidylyltransferase OS=Clostridium sp. Maddingley MBC34-26 OX=1196322 GN=A370_05500 PE=4 SV=1
MM1 pKa = 7.83SDD3 pKa = 3.17YY4 pKa = 11.88SMDD7 pKa = 2.86ISGNIEE13 pKa = 3.72LSDD16 pKa = 3.54YY17 pKa = 11.84SNIFDD22 pKa = 4.24YY23 pKa = 11.56LNIIDD28 pKa = 4.78KK29 pKa = 10.77DD30 pKa = 4.12DD31 pKa = 3.86NFVIRR36 pKa = 11.84IDD38 pKa = 3.91KK39 pKa = 10.44NNKK42 pKa = 8.97NDD44 pKa = 3.27INIINTMLKK53 pKa = 10.43DD54 pKa = 3.53NQFVISYY61 pKa = 10.86AEE63 pKa = 3.77YY64 pKa = 10.89DD65 pKa = 3.66DD66 pKa = 4.41FGNYY70 pKa = 8.57YY71 pKa = 9.67ICANKK76 pKa = 10.29DD77 pKa = 3.16SSKK80 pKa = 11.56NNII83 pKa = 3.49

Molecular weight: 9.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K6UC12|K6UC12_9CLOT Uncharacterized protein OS=Clostridium sp. Maddingley MBC34-26 OX=1196322 GN=A370_00302 PE=4 SV=1
MM1 pKa = 7.61SRR3 pKa = 11.84EE4 pKa = 3.76EE5 pKa = 4.22GNNNRR10 pKa = 11.84RR11 pKa = 11.84PGGKK15 pKa = 8.05MRR17 pKa = 11.84RR18 pKa = 11.84SRR20 pKa = 11.84KK21 pKa = 9.11KK22 pKa = 8.92VCAFCVDD29 pKa = 3.07KK30 pKa = 11.69AEE32 pKa = 4.83FIDD35 pKa = 4.01YY36 pKa = 11.0KK37 pKa = 11.0DD38 pKa = 3.24INKK41 pKa = 9.06LRR43 pKa = 11.84KK44 pKa = 9.21YY45 pKa = 7.83VTEE48 pKa = 4.22RR49 pKa = 11.84GKK51 pKa = 10.15ILPRR55 pKa = 11.84RR56 pKa = 11.84ISGTCAKK63 pKa = 9.87HH64 pKa = 5.43QRR66 pKa = 11.84EE67 pKa = 4.29LTASIKK73 pKa = 9.88RR74 pKa = 11.84ARR76 pKa = 11.84NIALLPFTTEE86 pKa = 3.59

Molecular weight: 10.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5778

0

5778

1727297

20

3038

298.9

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.728 ± 0.034

1.201 ± 0.013

5.58 ± 0.03

7.398 ± 0.042

4.39 ± 0.029

6.293 ± 0.033

1.366 ± 0.014

9.916 ± 0.041

8.781 ± 0.031

8.977 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.55 ± 0.019

6.616 ± 0.034

2.76 ± 0.019

2.494 ± 0.016

3.244 ± 0.022

6.546 ± 0.029

4.947 ± 0.03

6.199 ± 0.03

0.798 ± 0.013

4.215 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski