Lake Sarah-associated circular virus-8

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQK1|A0A140AQK1_9VIRU Replication associated protein (Fragment) OS=Lake Sarah-associated circular virus-8 OX=1685785 PE=3 SV=1
PP1 pKa = 7.8ALRR4 pKa = 11.84ITPTHH9 pKa = 6.09MATNNKK15 pKa = 9.38FSLQAKK21 pKa = 9.6RR22 pKa = 11.84FFLTFPQCPMDD33 pKa = 3.45KK34 pKa = 10.43HH35 pKa = 6.6QVLLWLTGAHH45 pKa = 6.39EE46 pKa = 4.02AHH48 pKa = 5.8QAIVAEE54 pKa = 4.44EE55 pKa = 3.89QHH57 pKa = 6.59EE58 pKa = 4.58DD59 pKa = 3.68GSPHH63 pKa = 5.29LHH65 pKa = 6.94IYY67 pKa = 8.26LTYY70 pKa = 10.16EE71 pKa = 3.49KK72 pKa = 10.46AKK74 pKa = 10.19RR75 pKa = 11.84ISHH78 pKa = 7.04ADD80 pKa = 3.44YY81 pKa = 10.81FDD83 pKa = 4.36LGNPEE88 pKa = 3.96NPEE91 pKa = 4.03QVFHH95 pKa = 7.08PNIQTVKK102 pKa = 10.69NMKK105 pKa = 9.95EE106 pKa = 3.91CVKK109 pKa = 10.92YY110 pKa = 9.24ITKK113 pKa = 9.46TDD115 pKa = 3.63KK116 pKa = 11.03EE117 pKa = 4.27PAQHH121 pKa = 6.55NINYY125 pKa = 9.6RR126 pKa = 11.84DD127 pKa = 3.57IIAGKK132 pKa = 8.59SSKK135 pKa = 10.22FAVIAASVMDD145 pKa = 4.46GKK147 pKa = 11.22SLLEE151 pKa = 4.72INDD154 pKa = 3.72SDD156 pKa = 3.85PGFVLQHH163 pKa = 5.39KK164 pKa = 9.94RR165 pKa = 11.84KK166 pKa = 10.14LEE168 pKa = 4.13EE169 pKa = 3.68YY170 pKa = 9.8QAWIVMKK177 pKa = 10.64KK178 pKa = 9.77PRR180 pKa = 11.84NDD182 pKa = 2.98LAAWYY187 pKa = 6.72PVPVPAFGPEE197 pKa = 3.9YY198 pKa = 10.73QLATWLNANLFAQPRR213 pKa = 11.84RR214 pKa = 11.84IKK216 pKa = 10.66ASQLYY221 pKa = 10.26LFSPPNCGKK230 pKa = 7.34TTLITWLSRR239 pKa = 11.84FCRR242 pKa = 11.84IYY244 pKa = 11.17YY245 pKa = 9.11MPLLEE250 pKa = 5.4DD251 pKa = 5.42FYY253 pKa = 11.64DD254 pKa = 5.11FYY256 pKa = 11.8DD257 pKa = 4.62DD258 pKa = 5.23DD259 pKa = 6.06SYY261 pKa = 12.22DD262 pKa = 3.52LVVIDD267 pKa = 4.36EE268 pKa = 4.9FKK270 pKa = 10.88GQKK273 pKa = 9.49QIQFLNQWLDD283 pKa = 3.6GQPCTVRR290 pKa = 11.84IKK292 pKa = 10.69GGQRR296 pKa = 11.84LKK298 pKa = 10.15TRR300 pKa = 11.84NLPCIILSNWALSTVYY316 pKa = 10.27SAAIEE321 pKa = 4.07KK322 pKa = 10.41SGPDD326 pKa = 3.39VIGPLIARR334 pKa = 11.84LEE336 pKa = 4.06IVNVNKK342 pKa = 10.43FITFYY347 pKa = 11.29DD348 pKa = 4.08PVWVEE353 pKa = 3.96AQNKK357 pKa = 7.97EE358 pKa = 3.96NN359 pKa = 3.73

Molecular weight: 41.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQK1|A0A140AQK1_9VIRU Replication associated protein (Fragment) OS=Lake Sarah-associated circular virus-8 OX=1685785 PE=3 SV=1
MM1 pKa = 7.37NKK3 pKa = 7.99TWNTGHH9 pKa = 7.29ILPYY13 pKa = 10.43LSAAGTAVQLGWNAWKK29 pKa = 9.67NQGYY33 pKa = 10.0GSYY36 pKa = 9.53EE37 pKa = 3.81LSKK40 pKa = 10.98EE41 pKa = 4.19PEE43 pKa = 3.88KK44 pKa = 11.3KK45 pKa = 9.34MGRR48 pKa = 11.84FRR50 pKa = 11.84QRR52 pKa = 11.84KK53 pKa = 5.63RR54 pKa = 11.84QRR56 pKa = 11.84RR57 pKa = 11.84GRR59 pKa = 11.84FNVRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84KK66 pKa = 10.09SRR68 pKa = 11.84IGGLRR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84IRR77 pKa = 11.84KK78 pKa = 9.08INRR81 pKa = 11.84TLFTKK86 pKa = 10.14GVKK89 pKa = 10.09SIEE92 pKa = 3.74VKK94 pKa = 10.82YY95 pKa = 10.62RR96 pKa = 11.84FSTADD101 pKa = 3.23GRR103 pKa = 11.84STSSWSSASTWDD115 pKa = 4.26HH116 pKa = 5.74ITDD119 pKa = 3.47IATGSGPTSRR129 pKa = 11.84VGSKK133 pKa = 10.41VFLRR137 pKa = 11.84HH138 pKa = 5.59LNLRR142 pKa = 11.84MHH144 pKa = 6.01VRR146 pKa = 11.84ASKK149 pKa = 9.04TVPAEE154 pKa = 3.55NEE156 pKa = 3.75QYY158 pKa = 10.34VRR160 pKa = 11.84VMVVRR165 pKa = 11.84HH166 pKa = 5.67SNIQVTPNWSAVSSIPDD183 pKa = 3.57LNILFEE189 pKa = 4.41PALANDD195 pKa = 5.22LDD197 pKa = 4.27YY198 pKa = 11.87QMLPFKK204 pKa = 10.58RR205 pKa = 11.84INNRR209 pKa = 11.84YY210 pKa = 8.66SKK212 pKa = 10.46GFKK215 pKa = 9.81VLYY218 pKa = 10.14SKK220 pKa = 9.71MVKK223 pKa = 9.79VSKK226 pKa = 9.34EE227 pKa = 3.65TGADD231 pKa = 3.31YY232 pKa = 10.34EE233 pKa = 4.11QRR235 pKa = 11.84IWKK238 pKa = 9.7ARR240 pKa = 11.84IPIMKK245 pKa = 8.85PCQWGNTDD253 pKa = 2.9ATGITDD259 pKa = 3.76VGPGQIVIYY268 pKa = 10.05CWDD271 pKa = 3.91NEE273 pKa = 4.13PSALAADD280 pKa = 3.95HH281 pKa = 6.82PLVKK285 pKa = 10.65VGGRR289 pKa = 11.84LSWTDD294 pKa = 3.05VV295 pKa = 3.09

Molecular weight: 33.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

654

295

359

327.0

37.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.881 ± 0.453

1.223 ± 0.317

4.893 ± 0.479

4.434 ± 0.607

3.976 ± 0.734

5.505 ± 1.135

2.599 ± 0.329

6.422 ± 0.383

7.339 ± 0.069

7.645 ± 0.897

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.988 ± 0.224

5.505 ± 0.047

5.657 ± 0.727

4.587 ± 0.696

6.575 ± 1.891

6.269 ± 1.281

5.352 ± 0.436

6.422 ± 0.602

2.752 ± 0.37

3.976 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski