Lactococcus phage 98204

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8BMI3|A0A1P8BMI3_9CAUD Distal tail protein OS=Lactococcus phage 98204 OX=1868860 GN=DS98204_51 PE=4 SV=1
MM1 pKa = 7.37RR2 pKa = 11.84WEE4 pKa = 3.92MTNLYY9 pKa = 10.45DD10 pKa = 3.6EE11 pKa = 4.73TVTILEE17 pKa = 4.37SHH19 pKa = 7.5DD20 pKa = 3.43KK21 pKa = 10.26TIADD25 pKa = 3.41IEE27 pKa = 4.68YY28 pKa = 9.72IGSSEE33 pKa = 4.39TKK35 pKa = 10.27INTNKK40 pKa = 10.44ALEE43 pKa = 4.12LMKK46 pKa = 9.95KK47 pKa = 7.93TNYY50 pKa = 10.17DD51 pKa = 2.86SGYY54 pKa = 9.28GGQEE58 pKa = 2.95IAYY61 pKa = 10.2NLMIKK66 pKa = 10.65GNGFIMTRR74 pKa = 11.84GEE76 pKa = 4.1YY77 pKa = 10.23DD78 pKa = 3.15GSEE81 pKa = 3.54WWDD84 pKa = 3.55YY85 pKa = 9.77MQTDD89 pKa = 3.84PSLPQVEE96 pKa = 4.37RR97 pKa = 11.84DD98 pKa = 3.6VKK100 pKa = 10.74SFKK103 pKa = 11.3ANIGWDD109 pKa = 3.46SLEE112 pKa = 4.71GINGLEE118 pKa = 4.13DD119 pKa = 3.58TKK121 pKa = 11.71NDD123 pKa = 3.37

Molecular weight: 14.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8BME4|A0A1P8BME4_9CAUD Uncharacterized protein OS=Lactococcus phage 98204 OX=1868860 GN=DS98204_07 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.35FGMRR6 pKa = 11.84KK7 pKa = 9.38PSITKK12 pKa = 9.31ILKK15 pKa = 10.0ARR17 pKa = 11.84TTTKK21 pKa = 10.49YY22 pKa = 9.79KK23 pKa = 10.62RR24 pKa = 11.84KK25 pKa = 9.38VKK27 pKa = 10.28KK28 pKa = 10.44ALIPGYY34 pKa = 9.54GKK36 pKa = 10.74KK37 pKa = 10.71GMGWIKK43 pKa = 10.5NPKK46 pKa = 7.19KK47 pKa = 10.16AAYY50 pKa = 9.03IEE52 pKa = 4.37HH53 pKa = 6.51SRR55 pKa = 11.84HH56 pKa = 5.12

Molecular weight: 6.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12032

47

1356

214.9

24.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.416 ± 0.302

0.59 ± 0.096

6.15 ± 0.204

6.674 ± 0.593

4.513 ± 0.194

6.499 ± 0.594

1.388 ± 0.152

7.006 ± 0.283

8.951 ± 0.542

7.571 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.502 ± 0.157

6.533 ± 0.288

2.718 ± 0.215

4.388 ± 0.229

3.665 ± 0.247

6.558 ± 0.319

6.499 ± 0.282

5.768 ± 0.284

1.621 ± 0.162

3.989 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski