Bodo saltans virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Imitervirales; Mimiviridae; unclassified Mimiviridae; Klosneuvirinae; Klosneuvirus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1186 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4UTS5|A0A2H4UTS5_9VIRU Uncharacterized protein OS=Bodo saltans virus OX=2024608 GN=BMW23_0283 PE=4 SV=1
MM1 pKa = 7.27YY2 pKa = 10.6KK3 pKa = 10.08NDD5 pKa = 5.46KK6 pKa = 10.23IDD8 pKa = 4.67LNDD11 pKa = 3.79DD12 pKa = 3.75SIYY15 pKa = 10.85DD16 pKa = 3.77YY17 pKa = 11.22VIEE20 pKa = 4.64DD21 pKa = 4.0TLTSYY26 pKa = 10.45VPKK29 pKa = 10.4RR30 pKa = 11.84KK31 pKa = 9.65EE32 pKa = 3.77NEE34 pKa = 4.36DD35 pKa = 3.36IEE37 pKa = 4.69TFIDD41 pKa = 3.86DD42 pKa = 3.88SSNFEE47 pKa = 3.7IPFFYY52 pKa = 9.93MIFVMVILALVYY64 pKa = 10.49IFFIQQ69 pKa = 3.52

Molecular weight: 8.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4UUI7|A0A2H4UUI7_9VIRU Uncharacterized protein OS=Bodo saltans virus OX=2024608 GN=BMW23_0484 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.48AEE4 pKa = 4.23CTVGRR9 pKa = 11.84RR10 pKa = 11.84QVDD13 pKa = 2.94KK14 pKa = 10.58FQRR17 pKa = 11.84VDD19 pKa = 3.59PLRR22 pKa = 11.84GLYY25 pKa = 9.35ILKK28 pKa = 10.0RR29 pKa = 11.84YY30 pKa = 9.38IMRR33 pKa = 11.84AA34 pKa = 3.16

Molecular weight: 4.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1186

0

1186

387114

33

4840

326.4

38.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.761 ± 0.101

3.023 ± 0.099

6.183 ± 0.094

6.771 ± 0.115

4.539 ± 0.065

3.975 ± 0.108

2.438 ± 0.054

9.643 ± 0.123

9.991 ± 0.168

7.98 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.353 ± 0.048

9.828 ± 0.143

2.409 ± 0.047

3.026 ± 0.073

2.543 ± 0.051

5.761 ± 0.112

5.364 ± 0.191

3.867 ± 0.063

0.96 ± 0.031

5.584 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski