Pseudomonas phage Pf-10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Pifdecavirus; Pseudomonas virus Pf10

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0YX84|A0A0A0YX84_9CAUD Portal protein OS=Pseudomonas phage Pf-10 OX=1562076 GN=NL61_31 PE=3 SV=1
MM1 pKa = 7.06KK2 pKa = 10.22HH3 pKa = 5.97IVQAMGDD10 pKa = 3.65VQVYY14 pKa = 10.71NDD16 pKa = 4.14ADD18 pKa = 3.75NTFEE22 pKa = 4.31TLPHH26 pKa = 6.67PGPYY30 pKa = 10.07LVGASVRR37 pKa = 11.84EE38 pKa = 4.19DD39 pKa = 3.55GVVLAFVDD47 pKa = 4.37PTAMNDD53 pKa = 3.42TEE55 pKa = 4.14ALAWQGDD62 pKa = 3.35KK63 pKa = 10.18WVAVV67 pKa = 3.82

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0YRW8|A0A0A0YRW8_9CAUD Uncharacterized protein OS=Pseudomonas phage Pf-10 OX=1562076 GN=NL61_12 PE=4 SV=1
MM1 pKa = 7.32TLAINLVHH9 pKa = 6.99TIDD12 pKa = 4.11ASFATIEE19 pKa = 4.0RR20 pKa = 11.84RR21 pKa = 11.84AMGHH25 pKa = 5.18TLAEE29 pKa = 3.76ITGRR33 pKa = 11.84KK34 pKa = 6.86VRR36 pKa = 11.84KK37 pKa = 8.87PGLYY41 pKa = 9.79DD42 pKa = 4.62RR43 pKa = 11.84KK44 pKa = 9.43ITDD47 pKa = 3.53AKK49 pKa = 10.14RR50 pKa = 11.84GSIGATYY57 pKa = 10.13LAHH60 pKa = 7.55RR61 pKa = 11.84EE62 pKa = 3.9GKK64 pKa = 9.55RR65 pKa = 11.84AVMCMAYY72 pKa = 10.31GMRR75 pKa = 11.84PQDD78 pKa = 4.74DD79 pKa = 3.9LQQALDD85 pKa = 3.52ARR87 pKa = 11.84HH88 pKa = 5.91RR89 pKa = 11.84QPGFQGAQFFTEE101 pKa = 4.03RR102 pKa = 11.84GSFEE106 pKa = 4.05HH107 pKa = 6.53LAGRR111 pKa = 11.84GVV113 pKa = 3.03

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

12130

45

1331

263.7

29.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.206 ± 0.581

0.791 ± 0.156

6.397 ± 0.215

6.991 ± 0.367

3.611 ± 0.196

8.17 ± 0.364

2.234 ± 0.182

4.633 ± 0.148

6.035 ± 0.326

8.211 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.993 ± 0.233

3.817 ± 0.175

3.833 ± 0.193

4.254 ± 0.387

5.829 ± 0.244

5.326 ± 0.3

5.664 ± 0.249

6.777 ± 0.382

1.36 ± 0.155

2.869 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski