Eragrostis streak virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0Z3X7|B0Z3X7_9GEMI Capsid protein OS=Eragrostis streak virus OX=496807 PE=3 SV=1
MM1 pKa = 7.71EE2 pKa = 4.89SFEE5 pKa = 4.43GAGSFVPQVALPRR18 pKa = 11.84VPPAAPSVPSLPWSSVGEE36 pKa = 3.59IAIFIFVAVLALYY49 pKa = 9.25LLWIWVVRR57 pKa = 11.84DD58 pKa = 3.29CLFVVKK64 pKa = 10.42ARR66 pKa = 11.84QGGSTEE72 pKa = 3.95EE73 pKa = 4.08LQFGPRR79 pKa = 11.84EE80 pKa = 4.13RR81 pKa = 11.84PPVPAGDD88 pKa = 4.07RR89 pKa = 11.84PPLPPAVSVATTSEE103 pKa = 3.95TRR105 pKa = 11.84PFSVV109 pKa = 3.54

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0Z3X8|B0Z3X8_9GEMI Replication-associated protein OS=Eragrostis streak virus OX=496807 PE=3 SV=1
MM1 pKa = 7.89PLTAGKK7 pKa = 10.09RR8 pKa = 11.84KK9 pKa = 9.88RR10 pKa = 11.84SGDD13 pKa = 3.37ASSSKK18 pKa = 10.07VARR21 pKa = 11.84PKK23 pKa = 10.51LGRR26 pKa = 11.84SSAARR31 pKa = 11.84AGKK34 pKa = 9.18PSTRR38 pKa = 11.84ARR40 pKa = 11.84PSLQIQTLQAAGQSMISVPSGGVCDD65 pKa = 4.55LLGSFARR72 pKa = 11.84GSDD75 pKa = 2.95EE76 pKa = 4.47GNRR79 pKa = 11.84HH80 pKa = 4.54TNEE83 pKa = 3.6TVTYY87 pKa = 9.82KK88 pKa = 10.8VALDD92 pKa = 3.36YY93 pKa = 11.49HH94 pKa = 6.46FVATAGACKK103 pKa = 10.04YY104 pKa = 10.58SSIGTGVVWLVYY116 pKa = 10.04DD117 pKa = 4.3AQPQGNCPQVKK128 pKa = 10.28DD129 pKa = 3.98IFPHH133 pKa = 6.65PDD135 pKa = 2.96SLSAFPYY142 pKa = 6.97TWKK145 pKa = 10.5VGRR148 pKa = 11.84EE149 pKa = 3.83VCHH152 pKa = 6.21RR153 pKa = 11.84FVVKK157 pKa = 10.4RR158 pKa = 11.84RR159 pKa = 11.84WLFTMEE165 pKa = 4.01TNGRR169 pKa = 11.84IGSDD173 pKa = 3.29TPGPTSCWPPCRR185 pKa = 11.84KK186 pKa = 9.9NIYY189 pKa = 7.73FHH191 pKa = 7.04KK192 pKa = 10.44FEE194 pKa = 5.89AGLGVKK200 pKa = 7.97TEE202 pKa = 4.16WKK204 pKa = 8.16NTTGGDD210 pKa = 3.17VGDD213 pKa = 3.87IKK215 pKa = 10.91KK216 pKa = 10.21GALYY220 pKa = 10.14IVIAPGNGLEE230 pKa = 4.27FTVHH234 pKa = 5.73GNARR238 pKa = 11.84LYY240 pKa = 10.33FKK242 pKa = 10.94SVGNQQ247 pKa = 2.8

Molecular weight: 26.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

656

109

300

218.7

24.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.622 ± 0.974

1.372 ± 0.449

3.963 ± 0.736

5.335 ± 1.062

4.116 ± 0.801

6.86 ± 1.999

2.591 ± 0.944

4.268 ± 0.415

4.726 ± 1.329

6.86 ± 0.58

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.22 ± 0.107

2.896 ± 0.944

7.774 ± 2.253

4.573 ± 1.041

5.64 ± 0.551

9.299 ± 0.569

7.622 ± 1.553

7.47 ± 2.257

2.439 ± 0.18

3.354 ± 0.879
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski