Pseudogymnoascus sp. VKM F-4281 (FW-2241)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8727 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093ZXN9|A0A093ZXN9_9PEZI Uncharacterized protein OS=Pseudogymnoascus sp. VKM F-4281 (FW-2241) OX=1420906 GN=V493_06818 PE=4 SV=1
MM1 pKa = 7.54SGYY4 pKa = 11.32GNDD7 pKa = 4.31NNDD10 pKa = 2.79SSNRR14 pKa = 11.84GGDD17 pKa = 3.39SYY19 pKa = 11.9GSSNNNSSDD28 pKa = 3.69SYY30 pKa = 11.04GSSGNTRR37 pKa = 11.84SGDD40 pKa = 3.59NNDD43 pKa = 4.0SYY45 pKa = 11.9GSSNTRR51 pKa = 11.84SGDD54 pKa = 3.24NDD56 pKa = 3.92SYY58 pKa = 11.69GSSNTRR64 pKa = 11.84SGDD67 pKa = 3.28NDD69 pKa = 4.0SYY71 pKa = 11.98GSTNKK76 pKa = 10.04SSSDD80 pKa = 3.47SYY82 pKa = 11.39GSSNTRR88 pKa = 11.84SGDD91 pKa = 3.19NDD93 pKa = 3.13NSYY96 pKa = 11.4GSSNKK101 pKa = 9.81SSSNDD106 pKa = 3.04NDD108 pKa = 3.89SYY110 pKa = 11.96GG111 pKa = 3.28

Molecular weight: 11.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094CDS0|A0A094CDS0_9PEZI Uncharacterized protein (Fragment) OS=Pseudogymnoascus sp. VKM F-4281 (FW-2241) OX=1420906 GN=V493_07628 PE=4 SV=1
MM1 pKa = 7.67GPPIPPRR8 pKa = 11.84KK9 pKa = 8.69PPSVSIGSYY18 pKa = 10.25NRR20 pKa = 11.84IPPRR24 pKa = 11.84GPPQPARR31 pKa = 11.84PPFPPTQAQASHH43 pKa = 5.71SRR45 pKa = 11.84RR46 pKa = 11.84LHH48 pKa = 4.92QRR50 pKa = 11.84HH51 pKa = 6.23AFPPTPQHH59 pKa = 5.65PRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84SAPPLRR69 pKa = 11.84RR70 pKa = 11.84PHH72 pKa = 6.49KK73 pKa = 9.7RR74 pKa = 11.84RR75 pKa = 11.84HH76 pKa = 5.38HH77 pKa = 4.97QVPPRR82 pKa = 11.84IDD84 pKa = 3.15QLAQHH89 pKa = 6.11TTAQVQSRR97 pKa = 11.84HH98 pKa = 4.89RR99 pKa = 11.84RR100 pKa = 11.84QPRR103 pKa = 11.84QLLRR107 pKa = 11.84PEE109 pKa = 3.98RR110 pKa = 11.84AQRR113 pKa = 11.84GGHH116 pKa = 5.38QLQDD120 pKa = 3.39SPGLGKK126 pKa = 9.54HH127 pKa = 6.63HH128 pKa = 7.17IPAQPLHH135 pKa = 6.72APALL139 pKa = 3.97

Molecular weight: 15.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8727

0

8727

4351004

15

4962

498.6

54.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.942 ± 0.026

1.147 ± 0.009

5.715 ± 0.02

6.43 ± 0.025

3.557 ± 0.016

7.2 ± 0.024

2.238 ± 0.011

5.004 ± 0.019

5.083 ± 0.024

8.665 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.169 ± 0.009

3.737 ± 0.013

5.93 ± 0.03

3.883 ± 0.021

5.937 ± 0.019

8.109 ± 0.029

6.021 ± 0.017

6.145 ± 0.016

1.372 ± 0.008

2.717 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski