Escherichia phage APC_JM3.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GLI5|A0A2D1GLI5_9CAUD Serine/threonine protein phosphatase OS=Escherichia phage APC_JM3.2 OX=2045361 GN=APCJM32_9 PE=4 SV=1
MM1 pKa = 8.29DD2 pKa = 4.56YY3 pKa = 11.03SQLSDD8 pKa = 3.52FEE10 pKa = 5.23INVAVFEE17 pKa = 5.08AIHH20 pKa = 6.25NGSPDD25 pKa = 3.53YY26 pKa = 11.27KK27 pKa = 10.28EE28 pKa = 4.73GEE30 pKa = 3.99NGAMVFISFEE40 pKa = 4.02GDD42 pKa = 3.11IVNGDD47 pKa = 3.41AVEE50 pKa = 4.33VEE52 pKa = 4.34VEE54 pKa = 3.87RR55 pKa = 11.84GSFNPCANPADD66 pKa = 3.66AWPIITEE73 pKa = 4.09NNISIILDD81 pKa = 3.81NPSMPCATDD90 pKa = 3.23NARR93 pKa = 11.84DD94 pKa = 3.79LFDD97 pKa = 5.03DD98 pKa = 4.5AGPNVGVAYY107 pKa = 9.96DD108 pKa = 3.76NPLRR112 pKa = 11.84AAMIVFLMMQDD123 pKa = 3.31ANNAA127 pKa = 3.59

Molecular weight: 13.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GLQ7|A0A2D1GLQ7_9CAUD Spanin inner membrane subunit OS=Escherichia phage APC_JM3.2 OX=2045361 GN=APCJM32_13 PE=3 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84GLAYY6 pKa = 10.73NPGILPAEE14 pKa = 4.07MIIRR18 pKa = 11.84QRR20 pKa = 11.84VKK22 pKa = 10.19PMPSRR27 pKa = 11.84EE28 pKa = 3.93EE29 pKa = 3.67LLKK32 pKa = 10.96RR33 pKa = 11.84NSFPSVNQNKK43 pKa = 9.51YY44 pKa = 10.34LNAMWRR50 pKa = 11.84KK51 pKa = 9.48GGKK54 pKa = 7.24QQ55 pKa = 2.91

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11740

37

911

206.0

23.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.165 ± 0.601

1.21 ± 0.182

6.141 ± 0.24

7.121 ± 0.48

3.237 ± 0.192

7.095 ± 0.415

1.695 ± 0.154

6.133 ± 0.351

6.397 ± 0.359

7.266 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.888 ± 0.17

5.009 ± 0.298

3.978 ± 0.232

4.787 ± 0.434

5.937 ± 0.351

6.039 ± 0.401

5.077 ± 0.327

5.894 ± 0.325

1.508 ± 0.154

3.424 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski