Novosphingobium guangzhouense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5282 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K2FUE4|A0A2K2FUE4_9SPHN 3-isopropylmalate dehydratase small subunit OS=Novosphingobium guangzhouense OX=1850347 GN=leuD PE=3 SV=1
MM1 pKa = 7.26LVIGGIAGAAFAALTTGALPKK22 pKa = 10.39FADD25 pKa = 3.81PAANRR30 pKa = 11.84SVVYY34 pKa = 8.15EE35 pKa = 4.09APPAQRR41 pKa = 11.84SWLDD45 pKa = 3.32EE46 pKa = 4.1GLSALNSPAWPFGRR60 pKa = 11.84GAHH63 pKa = 5.88EE64 pKa = 4.21EE65 pKa = 4.31VPPPEE70 pKa = 4.89PPQDD74 pKa = 3.72YY75 pKa = 10.73APYY78 pKa = 10.41GYY80 pKa = 10.43GPPDD84 pKa = 5.44DD85 pKa = 5.24YY86 pKa = 11.94DD87 pKa = 5.62DD88 pKa = 5.26DD89 pKa = 4.1AWEE92 pKa = 3.73QRR94 pKa = 11.84QYY96 pKa = 11.74GNHH99 pKa = 6.65APYY102 pKa = 10.26QSEE105 pKa = 4.97DD106 pKa = 3.71YY107 pKa = 10.93ASSASAADD115 pKa = 4.05SQPLPALPRR124 pKa = 11.84ADD126 pKa = 4.01AADD129 pKa = 4.0DD130 pKa = 3.76AAARR134 pKa = 11.84AADD137 pKa = 3.72AAQDD141 pKa = 3.89VISAEE146 pKa = 4.26SAPP149 pKa = 3.72

Molecular weight: 15.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K2FUN2|A0A2K2FUN2_9SPHN Uncharacterized protein OS=Novosphingobium guangzhouense OX=1850347 GN=A8V01_08925 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.45GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 8.17VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84AKK41 pKa = 10.7LSAA44 pKa = 4.0

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5282

0

5282

1720164

29

2171

325.7

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.99 ± 0.052

0.838 ± 0.011

5.953 ± 0.025

5.424 ± 0.03

3.578 ± 0.019

8.934 ± 0.033

2.014 ± 0.019

4.865 ± 0.022

3.029 ± 0.025

9.735 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.016

2.627 ± 0.024

5.227 ± 0.027

3.196 ± 0.018

7.147 ± 0.034

5.417 ± 0.029

5.417 ± 0.031

7.296 ± 0.027

1.468 ± 0.014

2.339 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski