Pseudomonas phage vB_PaeP_YA3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TNL5|A0A4Y5TNL5_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_YA3 OX=2588095 PE=4 SV=1
MM1 pKa = 7.26YY2 pKa = 9.94KK3 pKa = 10.05HH4 pKa = 6.6ASISDD9 pKa = 3.92LLGKK13 pKa = 8.38TIKK16 pKa = 10.62QITGLEE22 pKa = 4.12AGSDD26 pKa = 3.75SVDD29 pKa = 4.29FEE31 pKa = 5.04CEE33 pKa = 3.87DD34 pKa = 3.56GSLFRR39 pKa = 11.84MYY41 pKa = 10.09HH42 pKa = 4.88QQDD45 pKa = 3.58CCEE48 pKa = 4.18SVSINDD54 pKa = 3.66IEE56 pKa = 6.26GDD58 pKa = 3.47TSDD61 pKa = 5.77LVGQPLVVAEE71 pKa = 4.78DD72 pKa = 3.82VSSEE76 pKa = 4.42DD77 pKa = 3.69FPAPPGYY84 pKa = 10.27YY85 pKa = 9.38VEE87 pKa = 4.88SYY89 pKa = 8.35TWTFYY94 pKa = 11.33RR95 pKa = 11.84LATAKK100 pKa = 10.31GFVVIRR106 pKa = 11.84WLGEE110 pKa = 3.9SNGYY114 pKa = 9.78YY115 pKa = 9.88SEE117 pKa = 4.87SVDD120 pKa = 3.84FSRR123 pKa = 11.84INN125 pKa = 3.02

Molecular weight: 13.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H0QS94|A0A6H0QS94_9CAUD Putative transcriptional repressor OS=Pseudomonas phage vB_PaeP_YA3 OX=2588095 PE=4 SV=1
MM1 pKa = 6.54TRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 10.02GKK8 pKa = 9.79AQSAPMDD15 pKa = 3.85GKK17 pKa = 10.62KK18 pKa = 10.13RR19 pKa = 11.84KK20 pKa = 8.91PDD22 pKa = 3.59PNGYY26 pKa = 9.75YY27 pKa = 10.41DD28 pKa = 3.34WLIGKK33 pKa = 9.63GYY35 pKa = 10.96APANAMRR42 pKa = 11.84MVEE45 pKa = 4.02AKK47 pKa = 10.5FGGEE51 pKa = 3.95AKK53 pKa = 10.32RR54 pKa = 11.84EE55 pKa = 3.98PSLGWAWKK63 pKa = 9.98GG64 pKa = 3.36

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

14134

20

847

217.4

23.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.299 ± 0.64

1.358 ± 0.206

5.837 ± 0.179

6.856 ± 0.471

3.092 ± 0.208

8.023 ± 0.369

1.698 ± 0.159

5.299 ± 0.263

4.302 ± 0.328

7.606 ± 0.357

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.207

3.389 ± 0.323

4.323 ± 0.223

4.38 ± 0.42

7.181 ± 0.366

6.983 ± 0.548

5.278 ± 0.376

5.936 ± 0.285

1.698 ± 0.142

2.915 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski