Faecalibacterium sp. CAG:74

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Faecalibacterium; environmental samples

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2464 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7I4K8|R7I4K8_9FIRM Bacterial SH3 domain protein OS=Faecalibacterium sp. CAG:74 OX=1262897 GN=BN770_00947 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.47KK3 pKa = 10.36FFSLFLAVLLIFSCWTAATAEE24 pKa = 4.32STDD27 pKa = 3.78AVSGAPLDD35 pKa = 3.73NDD37 pKa = 3.28IEE39 pKa = 4.42IVYY42 pKa = 10.21KK43 pKa = 10.12EE44 pKa = 4.76HH45 pKa = 7.01FDD47 pKa = 4.82DD48 pKa = 4.03MVTRR52 pKa = 11.84YY53 pKa = 10.27ADD55 pKa = 3.54EE56 pKa = 4.08LTEE59 pKa = 5.13AEE61 pKa = 4.75LLVSADD67 pKa = 3.52VYY69 pKa = 11.32DD70 pKa = 5.05AIGMIRR76 pKa = 11.84FDD78 pKa = 5.11SEE80 pKa = 4.05DD81 pKa = 3.25SLAARR86 pKa = 11.84QRR88 pKa = 11.84IYY90 pKa = 11.26GIASADD96 pKa = 3.75DD97 pKa = 3.36LHH99 pKa = 7.76AILTGYY105 pKa = 10.34FDD107 pKa = 5.55PNDD110 pKa = 3.54AAYY113 pKa = 8.5TADD116 pKa = 3.56AALDD120 pKa = 3.67ARR122 pKa = 11.84LQAILAACGLNPEE135 pKa = 5.24DD136 pKa = 4.38YY137 pKa = 10.43DD138 pKa = 3.83ISVIRR143 pKa = 11.84NLSGMPEE150 pKa = 5.19PITGTNWYY158 pKa = 7.34CTLIRR163 pKa = 11.84KK164 pKa = 9.26GIEE167 pKa = 3.85VAEE170 pKa = 4.91DD171 pKa = 3.19EE172 pKa = 4.62TNPYY176 pKa = 11.47DD177 pKa = 3.85MVIVLYY183 pKa = 10.38GDD185 pKa = 3.66EE186 pKa = 4.24MTVGAFVLNPEE197 pKa = 4.23VV198 pKa = 3.3

Molecular weight: 21.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7I6M8|R7I6M8_9FIRM Ribonuclease P protein component OS=Faecalibacterium sp. CAG:74 OX=1262897 GN=rnpA PE=3 SV=1
MM1 pKa = 7.25FRR3 pKa = 11.84TYY5 pKa = 9.97QPKK8 pKa = 9.54KK9 pKa = 7.56RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.87GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.78NGRR28 pKa = 11.84RR29 pKa = 11.84VLSARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.14SLTVV44 pKa = 3.2

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2464

0

2464

819612

29

1818

332.6

36.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.779 ± 0.06

1.606 ± 0.021

5.818 ± 0.04

6.231 ± 0.051

3.626 ± 0.027

7.322 ± 0.043

2.04 ± 0.026

5.523 ± 0.038

4.491 ± 0.044

9.915 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.292 ± 0.026

3.547 ± 0.03

4.24 ± 0.035

3.727 ± 0.033

5.613 ± 0.05

5.331 ± 0.038

6.021 ± 0.047

7.193 ± 0.045

1.173 ± 0.022

3.512 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski