Exophiala spinifera

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta;

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12047 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D1ZC81|A0A0D1ZC81_9EURO FAD-binding PCMH-type domain-containing protein OS=Exophiala spinifera OX=91928 GN=PV08_11596 PE=3 SV=1
MM1 pKa = 7.77TITGLLLAALAGTAIAQSSDD21 pKa = 3.11LVTVLNSTNGTATFSEE37 pKa = 4.79VLAEE41 pKa = 3.96IPGFLEE47 pKa = 5.01AIAGKK52 pKa = 8.34TNITVLAPNDD62 pKa = 3.55TAIAEE67 pKa = 4.43LLASPAGQALEE78 pKa = 5.39DD79 pKa = 3.98GGADD83 pKa = 3.39YY84 pKa = 11.52ALNLVLYY91 pKa = 9.48HH92 pKa = 6.55VIDD95 pKa = 3.95GGYY98 pKa = 10.98EE99 pKa = 4.02NITDD103 pKa = 3.91YY104 pKa = 11.67AVVPTLLTSSNYY116 pKa = 9.48TNVTGGQHH124 pKa = 6.42IGFYY128 pKa = 10.05WDD130 pKa = 3.85DD131 pKa = 4.44DD132 pKa = 4.21EE133 pKa = 6.65DD134 pKa = 4.23VVGFYY139 pKa = 10.74GGNDD143 pKa = 3.59FAPEE147 pKa = 3.97ASHH150 pKa = 6.48TPIPFDD156 pKa = 3.27QGWIYY161 pKa = 10.77VLNGVLQIPPSMSEE175 pKa = 4.15TVTSGDD181 pKa = 3.62FNGSSFVAALEE192 pKa = 4.08KK193 pKa = 10.88AGLKK197 pKa = 10.5EE198 pKa = 4.25EE199 pKa = 4.41FDD201 pKa = 4.39LLHH204 pKa = 7.07DD205 pKa = 3.63ATYY208 pKa = 9.72FVPVNDD214 pKa = 4.32GFDD217 pKa = 3.48AVEE220 pKa = 4.39DD221 pKa = 4.09ALSKK225 pKa = 11.11LSTEE229 pKa = 4.28EE230 pKa = 3.58LAEE233 pKa = 3.92VLKK236 pKa = 10.24YY237 pKa = 10.42HH238 pKa = 6.08VVAGKK243 pKa = 9.97VWDD246 pKa = 4.12FEE248 pKa = 4.48LLEE251 pKa = 4.52KK252 pKa = 10.73VSQVTTLQGKK262 pKa = 8.65NLTVTQTPDD271 pKa = 3.31EE272 pKa = 4.19EE273 pKa = 4.52WFINQAGIHH282 pKa = 4.82YY283 pKa = 8.6TDD285 pKa = 4.31LAVAGGVVLFIDD297 pKa = 4.08NVLNPLAADD306 pKa = 4.49FAAPRR311 pKa = 11.84NGTDD315 pKa = 4.11GEE317 pKa = 4.41GLPAFTVTNATSTSGTNDD335 pKa = 3.24STSSTASASPSIASATSTGLAAMPLSVGISTLIGGIAVALLFF377 pKa = 4.84

Molecular weight: 39.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D2BY59|A0A0D2BY59_9EURO T5orf172 domain-containing protein OS=Exophiala spinifera OX=91928 GN=PV08_06305 PE=4 SV=1
MM1 pKa = 7.89PDD3 pKa = 3.61DD4 pKa = 4.49VDD6 pKa = 4.33MKK8 pKa = 9.84STCNVDD14 pKa = 3.13GNQVTKK20 pKa = 11.32NKK22 pKa = 8.86MFSGNWMVVMEE33 pKa = 4.76GLTFAWMRR41 pKa = 11.84TFEE44 pKa = 4.38IVAGPPVTITSTPTATYY61 pKa = 8.99TITSTPRR68 pKa = 11.84ATVTTTSTDD77 pKa = 2.77IYY79 pKa = 10.13STTLSPLTVTVPSSTSTRR97 pKa = 11.84TITTTPKK104 pKa = 10.27QVTVDD109 pKa = 3.54STSTSTRR116 pKa = 11.84TRR118 pKa = 11.84ISRR121 pKa = 11.84TWSATVTTTTVTVSCQPQIVKK142 pKa = 10.09RR143 pKa = 11.84DD144 pKa = 3.75PTCTIRR150 pKa = 11.84PTKK153 pKa = 9.61ATLAAASNTTYY164 pKa = 9.78PAPRR168 pKa = 11.84GRR170 pKa = 11.84FFRR173 pKa = 11.84GAPRR177 pKa = 11.84RR178 pKa = 11.84SRR180 pKa = 11.84RR181 pKa = 11.84RR182 pKa = 11.84EE183 pKa = 3.72AFVEE187 pKa = 3.94PRR189 pKa = 11.84RR190 pKa = 11.84EE191 pKa = 3.83NQLFEE196 pKa = 5.0RR197 pKa = 11.84GAGTLLL203 pKa = 3.73

Molecular weight: 22.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12047

0

12047

5944843

49

5561

493.5

54.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.605 ± 0.016

1.219 ± 0.007

5.773 ± 0.014

6.004 ± 0.019

3.755 ± 0.012

6.868 ± 0.022

2.461 ± 0.011

4.836 ± 0.014

4.675 ± 0.018

8.989 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.158 ± 0.008

3.566 ± 0.01

5.924 ± 0.022

4.061 ± 0.015

6.165 ± 0.018

8.31 ± 0.023

6.084 ± 0.016

6.273 ± 0.016

1.491 ± 0.008

2.782 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski