Dromedary stool-associated circular ssDNA virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1ENW9|A0A0A1ENW9_9CIRC ATP-dependent helicase Rep OS=Dromedary stool-associated circular ssDNA virus OX=1574422 GN=rep PE=3 SV=1
MM1 pKa = 7.4SRR3 pKa = 11.84GKK5 pKa = 9.91RR6 pKa = 11.84WCFTINNYY14 pKa = 7.61TEE16 pKa = 4.63EE17 pKa = 4.18DD18 pKa = 3.23TSRR21 pKa = 11.84CEE23 pKa = 5.35RR24 pKa = 11.84IDD26 pKa = 3.6CEE28 pKa = 4.08YY29 pKa = 11.25LVFGKK34 pKa = 10.53EE35 pKa = 3.65IGEE38 pKa = 4.36EE39 pKa = 3.9EE40 pKa = 4.49HH41 pKa = 6.59TPHH44 pKa = 6.23LQGFIVFKK52 pKa = 10.63NRR54 pKa = 11.84KK55 pKa = 5.28TFNVVKK61 pKa = 10.65RR62 pKa = 11.84IIGEE66 pKa = 3.92NAHH69 pKa = 7.15IEE71 pKa = 4.17IARR74 pKa = 11.84GTVKK78 pKa = 10.3EE79 pKa = 3.77ASEE82 pKa = 4.31YY83 pKa = 10.17CKK85 pKa = 10.76KK86 pKa = 10.31EE87 pKa = 3.43GQYY90 pKa = 10.2FEE92 pKa = 5.42KK93 pKa = 11.22GEE95 pKa = 4.12LPPEE99 pKa = 3.92QNEE102 pKa = 4.34RR103 pKa = 11.84GGQATKK109 pKa = 10.6RR110 pKa = 11.84KK111 pKa = 9.33WEE113 pKa = 4.1EE114 pKa = 3.43TLKK117 pKa = 10.72AAKK120 pKa = 9.77EE121 pKa = 4.03GRR123 pKa = 11.84FDD125 pKa = 6.41DD126 pKa = 4.33IAPDD130 pKa = 3.58LYY132 pKa = 10.43IRR134 pKa = 11.84YY135 pKa = 9.24RR136 pKa = 11.84SSLKK140 pKa = 10.43AIYY143 pKa = 9.88QEE145 pKa = 4.13EE146 pKa = 4.55VNKK149 pKa = 8.49NTKK152 pKa = 10.11EE153 pKa = 3.79ITDD156 pKa = 4.15FDD158 pKa = 4.79LKK160 pKa = 11.35GHH162 pKa = 6.57FYY164 pKa = 10.24WIYY167 pKa = 10.65GPTGTGKK174 pKa = 9.65SHH176 pKa = 8.12LARR179 pKa = 11.84TMAASVDD186 pKa = 4.12PDD188 pKa = 3.71TPPYY192 pKa = 10.83LKK194 pKa = 10.66GLNKK198 pKa = 8.92WWSGYY203 pKa = 10.22KK204 pKa = 8.06MQKK207 pKa = 10.36AVIIEE212 pKa = 4.02EE213 pKa = 4.5ANPEE217 pKa = 4.17TCKK220 pKa = 10.94YY221 pKa = 9.79LAPLFKK227 pKa = 10.1QWCDD231 pKa = 2.7KK232 pKa = 10.78WPFTAEE238 pKa = 4.02TKK240 pKa = 10.6GGSFEE245 pKa = 4.5HH246 pKa = 7.43GIRR249 pKa = 11.84PQYY252 pKa = 10.99IFITSNYY259 pKa = 9.41SINEE263 pKa = 4.16CFPDD267 pKa = 4.32PNDD270 pKa = 3.61SEE272 pKa = 4.25PMKK275 pKa = 10.42RR276 pKa = 11.84RR277 pKa = 11.84CHH279 pKa = 6.08EE280 pKa = 3.88FFKK283 pKa = 10.66EE284 pKa = 4.89NKK286 pKa = 9.91DD287 pKa = 3.2SWIPLYY293 pKa = 10.38TDD295 pKa = 3.69NDD297 pKa = 4.04TQTFPIEE304 pKa = 4.08PVHH307 pKa = 6.91LGLNDD312 pKa = 3.43KK313 pKa = 10.51PIDD316 pKa = 3.48IGTQEE321 pKa = 4.52MPLLTPEE328 pKa = 4.94LEE330 pKa = 4.24VLEE333 pKa = 5.22DD334 pKa = 3.71GG335 pKa = 5.14

Molecular weight: 38.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1ENW9|A0A0A1ENW9_9CIRC ATP-dependent helicase Rep OS=Dromedary stool-associated circular ssDNA virus OX=1574422 GN=rep PE=3 SV=1
MM1 pKa = 7.82PNTVSFQVGTAYY13 pKa = 9.28TKK15 pKa = 10.17KK16 pKa = 9.71RR17 pKa = 11.84KK18 pKa = 9.63RR19 pKa = 11.84MPGTNNVNKK28 pKa = 10.79AEE30 pKa = 4.04IKK32 pKa = 9.35QWNPRR37 pKa = 11.84SFGEE41 pKa = 4.2GNTYY45 pKa = 10.86NGVNIWNVLDD55 pKa = 3.5KK56 pKa = 10.74TLWSVVNSSSWLPRR70 pKa = 11.84PIYY73 pKa = 10.17MPTQGSGSYY82 pKa = 8.66QRR84 pKa = 11.84IGSKK88 pKa = 10.02IFLKK92 pKa = 9.99YY93 pKa = 10.72LRR95 pKa = 11.84FKK97 pKa = 11.09GYY99 pKa = 10.27ISVFWRR105 pKa = 11.84NLIGVRR111 pKa = 11.84WRR113 pKa = 11.84LRR115 pKa = 11.84LLRR118 pKa = 11.84CDD120 pKa = 3.12NYY122 pKa = 11.0LFKK125 pKa = 10.73EE126 pKa = 4.48VTDD129 pKa = 3.86GTTAQAAIIQYY140 pKa = 11.08LDD142 pKa = 3.27LLKK145 pKa = 10.87NHH147 pKa = 6.43EE148 pKa = 4.69HH149 pKa = 6.14PTSGSEE155 pKa = 3.93EE156 pKa = 3.95WSVASTVWDD165 pKa = 3.49NCRR168 pKa = 11.84HH169 pKa = 5.28NFYY172 pKa = 11.05KK173 pKa = 10.46SVKK176 pKa = 9.88DD177 pKa = 3.67VNDD180 pKa = 3.68RR181 pKa = 11.84NVIRR185 pKa = 11.84SKK187 pKa = 11.0VIASGYY193 pKa = 9.77IPVSAPAEE201 pKa = 4.05LVTLGGALTGNAAGAITLSGYY222 pKa = 7.72NTTYY226 pKa = 10.75DD227 pKa = 3.42INNTRR232 pKa = 11.84YY233 pKa = 10.63AMPLDD238 pKa = 3.8VKK240 pKa = 9.63VKK242 pKa = 10.75CNDD245 pKa = 3.43YY246 pKa = 11.25VDD248 pKa = 5.64DD249 pKa = 4.24SVSYY253 pKa = 10.31YY254 pKa = 10.86YY255 pKa = 10.98VLEE258 pKa = 4.24TDD260 pKa = 3.65CGIGISYY267 pKa = 9.47TSANGAYY274 pKa = 10.14ASLATAGALFQLSFFIRR291 pKa = 11.84GYY293 pKa = 9.32FTDD296 pKa = 3.35SS297 pKa = 2.95

Molecular weight: 33.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

632

297

335

316.0

36.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.538 ± 0.821

1.899 ± 0.379

5.063 ± 0.24

7.12 ± 3.04

4.43 ± 0.499

7.278 ± 0.32

1.741 ± 0.502

6.329 ± 0.416

7.278 ± 1.299

6.487 ± 0.632

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.424 ± 0.053

6.171 ± 1.08

4.905 ± 1.056

2.69 ± 0.229

5.063 ± 0.222

6.171 ± 1.774

7.12 ± 0.197

5.222 ± 1.732

2.532 ± 0.111

5.538 ± 0.821
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski