Microbacterium phage Jacko

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5HCW5|A0A2Z5HCW5_9CAUD Uncharacterized protein OS=Microbacterium phage Jacko OX=2250370 GN=51 PE=4 SV=1
MM1 pKa = 7.25SWIGDD6 pKa = 3.39GDD8 pKa = 3.61IDD10 pKa = 5.13YY11 pKa = 9.25EE12 pKa = 4.25ALDD15 pKa = 4.2ASDD18 pKa = 4.52YY19 pKa = 11.32DD20 pKa = 3.82YY21 pKa = 11.53DD22 pKa = 4.44PYY24 pKa = 11.55YY25 pKa = 10.69DD26 pKa = 4.93DD27 pKa = 5.35PRR29 pKa = 11.84QEE31 pKa = 3.95DD32 pKa = 3.93EE33 pKa = 3.97RR34 pKa = 11.84WW35 pKa = 3.16

Molecular weight: 4.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5HD07|A0A2Z5HD07_9CAUD Uncharacterized protein OS=Microbacterium phage Jacko OX=2250370 GN=101 PE=4 SV=1
MM1 pKa = 7.34TMATPHH7 pKa = 6.27TCHH10 pKa = 6.92IDD12 pKa = 3.37CEE14 pKa = 4.68LIIEE18 pKa = 4.64GTRR21 pKa = 11.84KK22 pKa = 8.81IHH24 pKa = 5.64MMHH27 pKa = 6.78AAEE30 pKa = 4.75PSSAAGRR37 pKa = 11.84YY38 pKa = 7.2QWEE41 pKa = 4.12QNAKK45 pKa = 9.68ILAQGRR51 pKa = 11.84RR52 pKa = 11.84AWRR55 pKa = 11.84ATGQGQSRR63 pKa = 11.84ACRR66 pKa = 11.84VWDD69 pKa = 3.34YY70 pKa = 11.5LRR72 pKa = 11.84RR73 pKa = 11.84TDD75 pKa = 3.38APRR78 pKa = 11.84PRR80 pKa = 11.84VRR82 pKa = 11.84APRR85 pKa = 11.84RR86 pKa = 11.84FMAMVGSPWSDD97 pKa = 2.61EE98 pKa = 3.84HH99 pKa = 6.29TLRR102 pKa = 11.84TFNARR107 pKa = 11.84VRR109 pKa = 11.84EE110 pKa = 4.13MAEE113 pKa = 3.67EE114 pKa = 3.91AAVII118 pKa = 3.97

Molecular weight: 13.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

18315

35

1264

172.8

19.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.631 ± 0.41

0.453 ± 0.08

6.377 ± 0.224

7.704 ± 0.322

3.161 ± 0.152

8.397 ± 0.444

1.818 ± 0.164

4.903 ± 0.15

4.264 ± 0.326

7.131 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.165

3.62 ± 0.176

4.717 ± 0.32

3.145 ± 0.17

7.327 ± 0.254

5.924 ± 0.275

5.689 ± 0.245

7.355 ± 0.184

1.977 ± 0.153

3.047 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski