Rhodohalobacter sp. SW132

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Balneolaeota; Balneolia; Balneolales; Balneolaceae; Rhodohalobacter; unclassified Rhodohalobacter

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4092 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371QIZ6|A0A371QIZ6_9BACT SusC/RagA family TonB-linked outer membrane protein OS=Rhodohalobacter sp. SW132 OX=2293433 GN=DYD21_17495 PE=4 SV=1
MM1 pKa = 7.48RR2 pKa = 11.84QLFTVGIYY10 pKa = 9.92IATLILIASCTKK22 pKa = 10.42EE23 pKa = 3.82NPTNFNMEE31 pKa = 4.3TPQSVAVQDD40 pKa = 4.11SLDD43 pKa = 4.06SNFSPLKK50 pKa = 9.96TYY52 pKa = 10.87INTEE56 pKa = 3.61NSDD59 pKa = 3.96FKK61 pKa = 11.4LGTAVSASAFADD73 pKa = 3.17QGAIFGLVNSNFQEE87 pKa = 4.39VTVHH91 pKa = 6.64DD92 pKa = 4.4MSHH95 pKa = 6.27GAVVQADD102 pKa = 4.1GSHH105 pKa = 7.07DD106 pKa = 4.06LLSISDD112 pKa = 4.75LIGVAEE118 pKa = 3.94EE119 pKa = 4.6AGISVYY125 pKa = 10.97GNALISFEE133 pKa = 4.35NQNEE137 pKa = 4.18AYY139 pKa = 10.48LNDD142 pKa = 5.11LIAPEE147 pKa = 4.39TIEE150 pKa = 3.75VSEE153 pKa = 4.67PSWEE157 pKa = 4.65LISSADD163 pKa = 3.91FEE165 pKa = 4.74TDD167 pKa = 3.22DD168 pKa = 5.14DD169 pKa = 4.96SNYY172 pKa = 9.94EE173 pKa = 4.08ANEE176 pKa = 3.98GADD179 pKa = 3.82LSFTADD185 pKa = 3.18GEE187 pKa = 4.65GANGEE192 pKa = 4.08GRR194 pKa = 11.84ALQIVNAEE202 pKa = 3.92VRR204 pKa = 11.84EE205 pKa = 4.07NDD207 pKa = 2.97WDD209 pKa = 3.84SQFFMTFEE217 pKa = 4.67PNVEE221 pKa = 4.16EE222 pKa = 4.56GDD224 pKa = 3.55QLRR227 pKa = 11.84FVMDD231 pKa = 3.05VRR233 pKa = 11.84AEE235 pKa = 4.04QEE237 pKa = 3.95ASFPTQAHH245 pKa = 6.69DD246 pKa = 4.05APTEE250 pKa = 3.92YY251 pKa = 10.94LHH253 pKa = 7.05WDD255 pKa = 3.88FFGTINATPEE265 pKa = 3.88WSQHH269 pKa = 4.96LMEE272 pKa = 4.56ITVSEE277 pKa = 4.32EE278 pKa = 3.46QASAGTIAFNLGATATTYY296 pKa = 11.08YY297 pKa = 9.66FDD299 pKa = 4.76NMEE302 pKa = 3.45VWYY305 pKa = 10.68YY306 pKa = 8.83NTEE309 pKa = 3.95TGTEE313 pKa = 4.09LVEE316 pKa = 4.01KK317 pKa = 9.91TPEE320 pKa = 3.95EE321 pKa = 4.15KK322 pKa = 10.81EE323 pKa = 4.4EE324 pKa = 3.99ILSTEE329 pKa = 4.03LEE331 pKa = 4.25NWVSTMVSEE340 pKa = 5.23ASYY343 pKa = 10.95VDD345 pKa = 3.0AWDD348 pKa = 3.86VVSGTIEE355 pKa = 4.27GGDD358 pKa = 3.33EE359 pKa = 3.94NSFQLRR365 pKa = 11.84SDD367 pKa = 4.03GGFNWYY373 pKa = 9.67EE374 pKa = 3.71YY375 pKa = 10.44LGEE378 pKa = 4.44DD379 pKa = 4.02FGVQAFQVARR389 pKa = 11.84EE390 pKa = 3.99HH391 pKa = 7.06AGDD394 pKa = 3.83GDD396 pKa = 3.75ILFISDD402 pKa = 3.9YY403 pKa = 11.59GLDD406 pKa = 3.98NLDD409 pKa = 3.88KK410 pKa = 10.25THH412 pKa = 6.62GLINYY417 pKa = 8.95VEE419 pKa = 4.26YY420 pKa = 10.56IEE422 pKa = 5.37NNGAVVDD429 pKa = 5.16GIGTHH434 pKa = 5.69MNININSSRR443 pKa = 11.84QDD445 pKa = 2.87ITEE448 pKa = 3.94MFEE451 pKa = 4.69LLAATDD457 pKa = 4.45KK458 pKa = 10.69IIKK461 pKa = 10.0ISGLNVGLDD470 pKa = 4.2GISAGAASPEE480 pKa = 4.26VYY482 pKa = 9.67EE483 pKa = 4.87AQSEE487 pKa = 4.88MYY489 pKa = 10.46QFVADD494 pKa = 3.74QYY496 pKa = 11.03FSIVPEE502 pKa = 4.0SQRR505 pKa = 11.84YY506 pKa = 8.94GITIWNPLDD515 pKa = 4.46SSDD518 pKa = 4.19NPSGLWTSDD527 pKa = 3.36YY528 pKa = 10.61EE529 pKa = 4.47RR530 pKa = 11.84KK531 pKa = 9.57RR532 pKa = 11.84AYY534 pKa = 10.41AGFAVGLMNGFNSGNN549 pKa = 3.38

Molecular weight: 60.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371QHH9|A0A371QHH9_9BACT ATP-binding protein OS=Rhodohalobacter sp. SW132 OX=2293433 GN=DYD21_19700 PE=4 SV=1
AA1 pKa = 7.67AWQEE5 pKa = 3.77SGRR8 pKa = 11.84RR9 pKa = 11.84YY10 pKa = 10.04GSPRR14 pKa = 11.84IYY16 pKa = 10.74NQLRR20 pKa = 11.84KK21 pKa = 10.17DD22 pKa = 3.14GCTASRR28 pKa = 11.84PRR30 pKa = 11.84IARR33 pKa = 11.84LMVKK37 pKa = 9.75MGIASRR43 pKa = 11.84IRR45 pKa = 11.84KK46 pKa = 8.73KK47 pKa = 9.61WIKK50 pKa = 7.15TTHH53 pKa = 5.61STHH56 pKa = 5.79GWPVAANVLDD66 pKa = 4.61RR67 pKa = 11.84NFSPEE72 pKa = 3.48GLGQVWVSDD81 pKa = 3.01ITYY84 pKa = 10.15IRR86 pKa = 11.84GEE88 pKa = 4.19QGWMYY93 pKa = 11.15LL94 pKa = 3.63

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4092

0

4092

1445183

22

3562

353.2

39.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.69 ± 0.031

0.602 ± 0.011

6.312 ± 0.029

7.546 ± 0.038

4.759 ± 0.029

7.194 ± 0.043

2.133 ± 0.02

7.029 ± 0.032

4.928 ± 0.048

9.203 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.018

4.868 ± 0.028

4.06 ± 0.023

3.629 ± 0.025

5.139 ± 0.034

6.903 ± 0.038

5.537 ± 0.031

6.242 ± 0.027

1.288 ± 0.017

3.517 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski