Prevotella sp. AM34-19LB

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; unclassified Prevotella

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A414BEJ0|A0A414BEJ0_9BACT Uncharacterized protein OS=Prevotella sp. AM34-19LB OX=2292364 GN=DW830_13765 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 10.13QGKK5 pKa = 9.42IYY7 pKa = 10.49LYY9 pKa = 9.06NQYY12 pKa = 10.85VGLLTEE18 pKa = 5.13DD19 pKa = 3.19EE20 pKa = 4.57TGFTFAYY27 pKa = 10.32DD28 pKa = 3.76ADD30 pKa = 4.03YY31 pKa = 10.76LASDD35 pKa = 3.72GAEE38 pKa = 3.78AVSLTLPLSDD48 pKa = 4.41EE49 pKa = 4.86PYY51 pKa = 10.17HH52 pKa = 6.3DD53 pKa = 3.47TVLFPFFDD61 pKa = 3.93GLIPEE66 pKa = 4.81GWLLNIAEE74 pKa = 4.75SSWKK78 pKa = 9.98INQRR82 pKa = 11.84DD83 pKa = 3.71RR84 pKa = 11.84MSLLLACCKK93 pKa = 10.59DD94 pKa = 3.87CIGAVSVIPVEE105 pKa = 4.4EE106 pKa = 4.21PTSEE110 pKa = 4.04GTNASPQNN118 pKa = 3.57

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R6EWL9|A0A3R6EWL9_9BACT TolC family protein OS=Prevotella sp. AM34-19LB OX=2292364 GN=DW830_00200 PE=3 SV=1
MM1 pKa = 7.75ANTSCKK7 pKa = 9.85YY8 pKa = 9.57PSRR11 pKa = 11.84LRR13 pKa = 11.84LVHH16 pKa = 5.98THH18 pKa = 5.45TQNLYY23 pKa = 7.55PTTHH27 pKa = 6.94RR28 pKa = 11.84PSADD32 pKa = 2.58IRR34 pKa = 11.84LHH36 pKa = 4.99NRR38 pKa = 11.84CWTSAYY44 pKa = 10.41QLLCKK49 pKa = 9.74RR50 pKa = 11.84TTAVVRR56 pKa = 11.84LKK58 pKa = 10.58II59 pKa = 3.83

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2572

0

2572

830833

26

1801

323.0

36.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.245 ± 0.042

1.315 ± 0.018

5.836 ± 0.038

6.526 ± 0.046

4.437 ± 0.033

6.724 ± 0.045

2.077 ± 0.019

6.712 ± 0.042

7.354 ± 0.045

8.814 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.084 ± 0.021

5.075 ± 0.033

3.468 ± 0.023

3.744 ± 0.025

4.407 ± 0.033

5.96 ± 0.041

5.267 ± 0.033

6.361 ± 0.034

1.253 ± 0.02

4.343 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski