Alistipes sp. CAG:268

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; environmental samples

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6VQN4|R6VQN4_9BACT Uncharacterized protein OS=Alistipes sp. CAG:268 OX=1262693 GN=BN576_01836 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 9.92IKK4 pKa = 10.27RR5 pKa = 11.84YY6 pKa = 10.79AMMLAATTGLFAACQDD22 pKa = 3.79LDD24 pKa = 3.82EE25 pKa = 4.43MHH27 pKa = 7.04TYY29 pKa = 10.72APEE32 pKa = 4.2DD33 pKa = 3.4VVAPVLHH40 pKa = 6.44NLPEE44 pKa = 4.63EE45 pKa = 4.0IVITSEE51 pKa = 4.4NMGEE55 pKa = 4.3TQVFTWDD62 pKa = 3.24AADD65 pKa = 4.49FGVSTQINYY74 pKa = 10.32SLEE77 pKa = 4.22ASCGEE82 pKa = 4.03GDD84 pKa = 4.08PVVLFADD91 pKa = 3.99LTDD94 pKa = 3.74TSTEE98 pKa = 3.82QPYY101 pKa = 11.0EE102 pKa = 4.27SINTKK107 pKa = 10.08LVYY110 pKa = 10.2DD111 pKa = 4.57AGVEE115 pKa = 4.16PDD117 pKa = 3.93TPTQVNFYY125 pKa = 9.89ISATIGSDD133 pKa = 3.45FEE135 pKa = 4.5KK136 pKa = 10.55FYY138 pKa = 10.7SAPVAVTMTVTKK150 pKa = 10.59AEE152 pKa = 3.92RR153 pKa = 11.84VYY155 pKa = 9.5PTVWVIGDD163 pKa = 3.5YY164 pKa = 11.13CGWNHH169 pKa = 6.47SNSQFLFSFSDD180 pKa = 3.72DD181 pKa = 3.97EE182 pKa = 4.44INYY185 pKa = 10.45EE186 pKa = 3.93GMIDD190 pKa = 4.0FGEE193 pKa = 4.41KK194 pKa = 9.73AANGFKK200 pKa = 10.14LTGIGGWDD208 pKa = 4.05DD209 pKa = 3.54SCNWGTDD216 pKa = 3.49GEE218 pKa = 4.55AEE220 pKa = 4.07APEE223 pKa = 5.08AEE225 pKa = 4.18AASIKK230 pKa = 10.49LISAGNSGNITAYY243 pKa = 9.92SKK245 pKa = 10.6RR246 pKa = 11.84FYY248 pKa = 10.61RR249 pKa = 11.84FAFNRR254 pKa = 11.84EE255 pKa = 3.96SLTLTKK261 pKa = 10.26EE262 pKa = 3.86LSFDD266 pKa = 3.27QLGIVGDD273 pKa = 4.82GVGSWDD279 pKa = 3.48NDD281 pKa = 3.06VVMEE285 pKa = 4.95FDD287 pKa = 3.45TKK289 pKa = 7.84TQRR292 pKa = 11.84FWADD296 pKa = 3.07VEE298 pKa = 4.47LVSGAIKK305 pKa = 10.26FRR307 pKa = 11.84ADD309 pKa = 3.68GAWDD313 pKa = 3.56TSFGSSTEE321 pKa = 3.87GMLDD325 pKa = 3.24GSDD328 pKa = 4.2NIQVPAGNYY337 pKa = 9.48RR338 pKa = 11.84IYY340 pKa = 11.65VNLNNSAAMTYY351 pKa = 9.98EE352 pKa = 4.35LNADD356 pKa = 4.37DD357 pKa = 4.73YY358 pKa = 9.77GQSGGEE364 pKa = 4.03EE365 pKa = 4.13EE366 pKa = 5.17PDD368 pKa = 3.56PEE370 pKa = 4.07TADD373 pKa = 3.47WYY375 pKa = 10.4IHH377 pKa = 5.54GQTVATPDD385 pKa = 3.14WGPTPMEE392 pKa = 4.35SASSNIVAYY401 pKa = 9.46KK402 pKa = 9.92AAGVEE407 pKa = 4.42VAANSQFLFKK417 pKa = 10.94SGDD420 pKa = 3.24EE421 pKa = 4.4SQWLGADD428 pKa = 3.33ASLAGGDD435 pKa = 3.72EE436 pKa = 4.75MYY438 pKa = 10.0TCTVGSAFAVSANKK452 pKa = 9.81VNAVIAEE459 pKa = 4.24AGTYY463 pKa = 9.84DD464 pKa = 3.71YY465 pKa = 11.01WLLPEE470 pKa = 4.81AGRR473 pKa = 11.84AYY475 pKa = 11.1VMAAGVKK482 pKa = 8.97PEE484 pKa = 4.12YY485 pKa = 10.38VAEE488 pKa = 4.08TWGLIGTISGWGDD501 pKa = 3.32LGDD504 pKa = 4.13LSMSEE509 pKa = 3.84QEE511 pKa = 4.51GYY513 pKa = 10.45LVRR516 pKa = 11.84KK517 pKa = 9.63GVALTTADD525 pKa = 3.29EE526 pKa = 4.45FKK528 pKa = 10.8IRR530 pKa = 11.84FNNEE534 pKa = 2.8WDD536 pKa = 3.44DD537 pKa = 3.79SKK539 pKa = 11.94NYY541 pKa = 8.15GTASGGAIDD550 pKa = 4.8INTAVAVVTSGGSQNMKK567 pKa = 9.62VQLDD571 pKa = 3.92GTYY574 pKa = 10.42DD575 pKa = 3.12IYY577 pKa = 11.43FDD579 pKa = 4.38LANSTIYY586 pKa = 11.11VMSPGKK592 pKa = 8.89TPADD596 pKa = 3.63VQQ598 pKa = 3.61

Molecular weight: 64.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6VCK5|R6VCK5_9BACT Uncharacterized protein OS=Alistipes sp. CAG:268 OX=1262693 GN=BN576_01531 PE=4 SV=1
MM1 pKa = 7.84PNGKK5 pKa = 8.55KK6 pKa = 9.72HH7 pKa = 6.1KK8 pKa = 7.52RR9 pKa = 11.84HH10 pKa = 5.93KK11 pKa = 9.95MATHH15 pKa = 6.02KK16 pKa = 10.34RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.26NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.87KK28 pKa = 9.36KK29 pKa = 10.77

Molecular weight: 3.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2200

0

2200

781890

29

1639

355.4

39.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.469 ± 0.063

1.34 ± 0.02

5.699 ± 0.04

6.722 ± 0.047

4.161 ± 0.032

7.835 ± 0.047

1.921 ± 0.028

5.556 ± 0.039

3.889 ± 0.054

9.385 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.517 ± 0.023

3.547 ± 0.045

4.333 ± 0.032

3.008 ± 0.029

7.226 ± 0.064

5.655 ± 0.041

5.54 ± 0.041

7.078 ± 0.05

1.293 ± 0.024

3.822 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski