Flavobacterium phage FCL-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ficleduovirus; Flavobacterium virus FCL2

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0YT00|A0A0A0YT00_9CAUD Uncharacterized protein OS=Flavobacterium phage FCL-2 OX=908819 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.61VLGNLPIIKK11 pKa = 9.56DD12 pKa = 3.72VNSVYY17 pKa = 10.69PFGAAIKK24 pKa = 9.82NQTEE28 pKa = 4.53TEE30 pKa = 4.02QGTPAIRR37 pKa = 11.84EE38 pKa = 4.08IYY40 pKa = 10.6NDD42 pKa = 3.29VLMNIYY48 pKa = 10.31KK49 pKa = 10.03ILQLTGVNPSNEE61 pKa = 3.68EE62 pKa = 3.85DD63 pKa = 3.45NNYY66 pKa = 10.67NGYY69 pKa = 10.31QIIEE73 pKa = 4.19ALKK76 pKa = 10.49KK77 pKa = 10.72LPNSLNDD84 pKa = 3.17IEE86 pKa = 5.65QILTLDD92 pKa = 3.66GTVWTVPFDD101 pKa = 4.26LQYY104 pKa = 11.47LPNKK108 pKa = 9.86YY109 pKa = 9.25MFVARR114 pKa = 11.84ASEE117 pKa = 4.25NYY119 pKa = 9.99NPAITYY125 pKa = 6.92TFKK128 pKa = 11.25GLGVTEE134 pKa = 5.19LPFNPINGFSATDD147 pKa = 3.52EE148 pKa = 4.28LLVIIDD154 pKa = 3.73TSGVRR159 pKa = 11.84SYY161 pKa = 11.74SLNKK165 pKa = 8.12PTEE168 pKa = 4.14ASSIVYY174 pKa = 8.84TGTGQVVTFNDD185 pKa = 3.92LNTVYY190 pKa = 10.68YY191 pKa = 10.15YY192 pKa = 11.54NNNFSSGWLVADD204 pKa = 4.72TPNSCDD210 pKa = 2.75ILNIVRR216 pKa = 11.84LFASRR221 pKa = 11.84ATLEE225 pKa = 3.88IKK227 pKa = 10.62DD228 pKa = 3.68IFIHH232 pKa = 6.48NNTIVCFCFDD242 pKa = 4.6PSDD245 pKa = 3.53VAYY248 pKa = 10.0FIYY251 pKa = 10.73DD252 pKa = 3.16VDD254 pKa = 4.15LTKK257 pKa = 10.42LTVSFNIEE265 pKa = 3.96LSNDD269 pKa = 2.99ADD271 pKa = 3.91SNYY274 pKa = 10.95DD275 pKa = 3.14PVAYY279 pKa = 9.49MDD281 pKa = 4.07EE282 pKa = 5.11NGDD285 pKa = 2.95LWMTNRR291 pKa = 11.84GNLNGGVNDD300 pKa = 3.46NEE302 pKa = 4.27VYY304 pKa = 10.7KK305 pKa = 10.25FVRR308 pKa = 11.84NGLGIFTFNSTITLGVSFVKK328 pKa = 9.87TRR330 pKa = 11.84NAAIKK335 pKa = 10.33HH336 pKa = 5.27GFLYY340 pKa = 9.7THH342 pKa = 7.19FEE344 pKa = 4.32LYY346 pKa = 10.35LNKK349 pKa = 10.46YY350 pKa = 9.77NLTTGALTSSVYY362 pKa = 10.2FPAIGQLMQLNGEE375 pKa = 4.42VYY377 pKa = 10.63FNSGYY382 pKa = 8.9IAKK385 pKa = 10.25RR386 pKa = 11.84FDD388 pKa = 3.2LL389 pKa = 4.72

Molecular weight: 43.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0YQ72|A0A0A0YQ72_9CAUD Uncharacterized protein OS=Flavobacterium phage FCL-2 OX=908819 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84AWNANLRR9 pKa = 11.84LKK11 pKa = 10.72ANRR14 pKa = 11.84VVYY17 pKa = 10.17RR18 pKa = 11.84AYY20 pKa = 8.46THH22 pKa = 6.43NMVKK26 pKa = 8.85PTGTTDD32 pKa = 3.4YY33 pKa = 10.89VSVSFVLPVKK43 pKa = 10.66SDD45 pKa = 3.41FLANGGLLYY54 pKa = 10.87VNPNTGITITNRR66 pKa = 11.84YY67 pKa = 7.56IAAGLLHH74 pKa = 6.79KK75 pKa = 10.68GVV77 pKa = 3.71

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14454

41

841

195.3

22.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.237 ± 0.459

0.941 ± 0.116

5.825 ± 0.247

7.23 ± 0.452

4.725 ± 0.175

5.521 ± 0.293

1.335 ± 0.128

7.991 ± 0.256

9.202 ± 0.406

8.655 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.159

7.292 ± 0.298

2.927 ± 0.161

3.667 ± 0.192

3.722 ± 0.206

6.337 ± 0.281

5.978 ± 0.248

5.825 ± 0.231

1.072 ± 0.127

4.283 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski