Piscirickettsia litoralis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Piscirickettsia

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3GEB7|A0A1E3GEB7_9GAMM Arsenate reductase OS=Piscirickettsia litoralis OX=1891921 GN=BGC07_01175 PE=3 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84FEE4 pKa = 4.3GQEE7 pKa = 3.69PALFSSKK14 pKa = 8.55TLYY17 pKa = 10.93SLAAHH22 pKa = 6.58QIPADD27 pKa = 4.17SIAIDD32 pKa = 3.83ALSCDD37 pKa = 3.82EE38 pKa = 5.56LEE40 pKa = 4.6GLTLSFNDD48 pKa = 3.81GLIAVIDD55 pKa = 3.86WQQLYY60 pKa = 9.7PLSLNQQEE68 pKa = 4.19

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3GAT3|A0A1E3GAT3_9GAMM MFS domain-containing protein OS=Piscirickettsia litoralis OX=1891921 GN=BGC07_04920 PE=4 SV=1
MM1 pKa = 7.64AKK3 pKa = 9.71VCMIQRR9 pKa = 11.84EE10 pKa = 4.43EE11 pKa = 3.78KK12 pKa = 10.22RR13 pKa = 11.84ARR15 pKa = 11.84TVAKK19 pKa = 10.21YY20 pKa = 10.28AEE22 pKa = 4.06KK23 pKa = 10.41RR24 pKa = 11.84AALKK28 pKa = 10.73AIINDD33 pKa = 3.87LNASDD38 pKa = 4.14EE39 pKa = 4.49EE40 pKa = 4.35RR41 pKa = 11.84WDD43 pKa = 3.73AQVKK47 pKa = 9.19LQALPRR53 pKa = 11.84DD54 pKa = 3.85ASPVRR59 pKa = 11.84QQRR62 pKa = 11.84RR63 pKa = 11.84CRR65 pKa = 11.84ITGRR69 pKa = 11.84PHH71 pKa = 5.93AVYY74 pKa = 10.28RR75 pKa = 11.84KK76 pKa = 9.93FGLCRR81 pKa = 11.84NKK83 pKa = 10.13LRR85 pKa = 11.84QHH87 pKa = 6.11VMFGDD92 pKa = 3.75VPGVRR97 pKa = 11.84KK98 pKa = 10.13ASWW101 pKa = 2.78

Molecular weight: 11.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3188

0

3188

810863

28

1932

254.3

28.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.979 ± 0.051

1.169 ± 0.017

5.112 ± 0.039

5.861 ± 0.05

4.193 ± 0.032

6.283 ± 0.053

2.362 ± 0.026

7.066 ± 0.046

6.548 ± 0.052

10.367 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.024

4.954 ± 0.042

3.63 ± 0.026

4.639 ± 0.042

4.183 ± 0.036

7.113 ± 0.038

5.292 ± 0.051

6.347 ± 0.041

1.066 ± 0.019

3.517 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski