White sucker hepatitis B virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Parahepadnavirus

Average proteome isoelectric point is 9.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M3YV36|A0A0M3YV36_9HEPA DNA-directed DNA polymerase OS=White sucker hepatitis B virus OX=1690672 GN=wsHBV_gp2 PE=3 SV=1
MM1 pKa = 7.51GKK3 pKa = 10.41GSSKK7 pKa = 10.95LMTYY11 pKa = 10.57EE12 pKa = 3.88EE13 pKa = 4.51AQHH16 pKa = 4.73QRR18 pKa = 11.84RR19 pKa = 11.84MEE21 pKa = 3.88QYY23 pKa = 8.65MDD25 pKa = 3.41QQARR29 pKa = 11.84TALQRR34 pKa = 11.84LVSQSPARR42 pKa = 11.84DD43 pKa = 3.52VNKK46 pKa = 9.81TKK48 pKa = 10.72SLQWPKK54 pKa = 10.91LPDD57 pKa = 3.62PVTIHH62 pKa = 6.38KK63 pKa = 10.02PSYY66 pKa = 9.55QGSVPPKK73 pKa = 9.87RR74 pKa = 11.84PRR76 pKa = 11.84TEE78 pKa = 3.88LDD80 pKa = 3.0QPLDD84 pKa = 3.78HH85 pKa = 6.29QQSVPLQAPQTVLQPLEE102 pKa = 4.07PPPRR106 pKa = 11.84VMQIEE111 pKa = 4.44QPLQSPPLASKK122 pKa = 10.38VSPLSPIKK130 pKa = 10.27AVPFLAPLLIPVAHH144 pKa = 4.66QTVRR148 pKa = 11.84TSYY151 pKa = 11.83NMTTSNISEE160 pKa = 4.16IMDD163 pKa = 3.7EE164 pKa = 4.09ARR166 pKa = 11.84TAFTTMGSAFQGLSFDD182 pKa = 3.41LWVLFLVVLLVVFFLLIKK200 pKa = 10.17IHH202 pKa = 6.74TILKK206 pKa = 9.56AADD209 pKa = 2.67WWLISRR215 pKa = 11.84SFLGTQTCPFRR226 pKa = 11.84NTEE229 pKa = 4.43SPTSMHH235 pKa = 6.87FKK237 pKa = 9.69TDD239 pKa = 3.37CPLTCTGFRR248 pKa = 11.84WTLVRR253 pKa = 11.84RR254 pKa = 11.84SIIFLCILALVVIFWYY270 pKa = 10.67LMAWEE275 pKa = 4.92PFTAFVKK282 pKa = 10.61RR283 pKa = 11.84LWEE286 pKa = 4.42LGSVLGSYY294 pKa = 9.88IFSHH298 pKa = 7.04LSSQLGSVPTIQFIALLTWMIFYY321 pKa = 10.53SHH323 pKa = 6.72IPVWLHH329 pKa = 5.62FLLSFVTSWHH339 pKa = 5.96FLQNGGFF346 pKa = 3.55

Molecular weight: 39.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M3P4Q2|A0A0M3P4Q2_9HEPA Large envelope protein OS=White sucker hepatitis B virus OX=1690672 GN=wsHBV_gp3 PE=4 SV=1
MM1 pKa = 7.16FAFVLILLRR10 pKa = 11.84LWSGCLNGSSTMDD23 pKa = 4.19PLVKK27 pKa = 10.27AALEE31 pKa = 4.26GVKK34 pKa = 10.3DD35 pKa = 3.88LPHH38 pKa = 7.5DD39 pKa = 4.28FFPLLKK45 pKa = 10.77DD46 pKa = 3.26QVQFSKK52 pKa = 11.27DD53 pKa = 3.33VIKK56 pKa = 10.35EE57 pKa = 4.08YY58 pKa = 10.99NEE60 pKa = 3.75HH61 pKa = 6.72HH62 pKa = 5.95SQNRR66 pKa = 11.84HH67 pKa = 4.16VEE69 pKa = 4.36VINCSLEE76 pKa = 4.53SYY78 pKa = 11.25DD79 pKa = 4.39NLQLIYY85 pKa = 10.98AKK87 pKa = 9.37VTISNWTAIANNGDD101 pKa = 3.98SVQVALDD108 pKa = 3.52EE109 pKa = 4.17NARR112 pKa = 11.84TGLAYY117 pKa = 9.76IFQSTIEE124 pKa = 3.73MSIRR128 pKa = 11.84RR129 pKa = 11.84KK130 pKa = 9.91LWWHH134 pKa = 5.95TNCLMWGEE142 pKa = 4.41SQTAEE147 pKa = 4.02YY148 pKa = 8.24TAKK151 pKa = 10.31LRR153 pKa = 11.84SWLMTPTSYY162 pKa = 11.07RR163 pKa = 11.84NQYY166 pKa = 10.6APTIEE171 pKa = 4.11ALTRR175 pKa = 11.84TVKK178 pKa = 9.51ATHH181 pKa = 6.39LSSRR185 pKa = 11.84SSRR188 pKa = 11.84PTARR192 pKa = 11.84SPGVRR197 pKa = 11.84ARR199 pKa = 11.84SSSKK203 pKa = 10.75SKK205 pKa = 10.64SKK207 pKa = 10.62SPRR210 pKa = 11.84RR211 pKa = 11.84KK212 pKa = 7.49TT213 pKa = 3.29

Molecular weight: 24.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1347

213

788

449.0

50.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.35 ± 0.891

1.633 ± 0.236

3.267 ± 0.24

2.747 ± 0.584

4.826 ± 0.559

4.232 ± 0.485

3.638 ± 0.454

4.9 ± 0.228

5.048 ± 0.462

11.359 ± 0.485

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.856 ± 0.633

3.192 ± 0.625

7.275 ± 0.702

4.677 ± 0.86

5.271 ± 0.356

10.69 ± 0.635

6.904 ± 0.359

5.939 ± 0.389

2.153 ± 0.538

3.044 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski