Porcine feces-associated gemycircularvirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus anima1

Average proteome isoelectric point is 8.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221LEA2|A0A221LEA2_9VIRU Capsid OS=Porcine feces-associated gemycircularvirus OX=2017712 PE=4 SV=1
MM1 pKa = 6.73VQYY4 pKa = 10.71NVNSWTIRR12 pKa = 11.84PFRR15 pKa = 11.84KK16 pKa = 9.8LMSSFRR22 pKa = 11.84FQARR26 pKa = 11.84YY27 pKa = 10.11GLFTYY32 pKa = 7.58SQCGRR37 pKa = 11.84LRR39 pKa = 11.84PQQVSEE45 pKa = 4.48HH46 pKa = 5.12FTSLGADD53 pKa = 3.66HH54 pKa = 7.29IIGRR58 pKa = 11.84EE59 pKa = 3.79THH61 pKa = 5.99QDD63 pKa = 3.12GGTHH67 pKa = 5.9LHH69 pKa = 5.88VFADD73 pKa = 4.56FRR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 9.94YY78 pKa = 9.02RR79 pKa = 11.84TRR81 pKa = 11.84DD82 pKa = 3.02NRR84 pKa = 11.84KK85 pKa = 8.04FDD87 pKa = 3.6VEE89 pKa = 4.33GFHH92 pKa = 6.93PNIVPSLRR100 pKa = 11.84NPAGGWDD107 pKa = 3.84YY108 pKa = 10.92ATKK111 pKa = 10.83DD112 pKa = 3.2GDD114 pKa = 3.43ICAGEE119 pKa = 4.27LSRR122 pKa = 11.84PDD124 pKa = 3.67DD125 pKa = 3.77EE126 pKa = 5.41RR127 pKa = 11.84GSRR130 pKa = 11.84SRR132 pKa = 11.84AADD135 pKa = 3.74AGWDD139 pKa = 3.53ALRR142 pKa = 11.84AAEE145 pKa = 4.19TRR147 pKa = 11.84DD148 pKa = 3.33DD149 pKa = 3.96FFRR152 pKa = 11.84IASEE156 pKa = 4.06HH157 pKa = 6.19LFSHH161 pKa = 6.53LVKK164 pKa = 10.71SFNSFSAYY172 pKa = 10.37ADD174 pKa = 2.82WKK176 pKa = 10.51FRR178 pKa = 11.84VDD180 pKa = 3.55RR181 pKa = 11.84TPYY184 pKa = 9.52IHH186 pKa = 7.59DD187 pKa = 4.32PNVTFDD193 pKa = 4.76LGQLHH198 pKa = 6.29QLRR201 pKa = 11.84EE202 pKa = 4.47WIRR205 pKa = 11.84GNLPPGNSKK214 pKa = 10.32YY215 pKa = 9.04RR216 pKa = 11.84TLHH219 pKa = 6.14LRR221 pKa = 11.84VQSIEE226 pKa = 3.65LRR228 pKa = 11.84EE229 pKa = 3.99RR230 pKa = 11.84GISLLLWGRR239 pKa = 11.84SRR241 pKa = 11.84TGKK244 pKa = 7.69TWLARR249 pKa = 11.84SFGSHH254 pKa = 6.83CYY256 pKa = 10.22FGGLFALDD264 pKa = 3.44EE265 pKa = 4.33YY266 pKa = 11.04EE267 pKa = 4.67YY268 pKa = 11.33NDD270 pKa = 3.63KK271 pKa = 10.87VDD273 pKa = 3.48YY274 pKa = 11.18AVFDD278 pKa = 5.21DD279 pKa = 3.75IQGGFKK285 pKa = 10.57YY286 pKa = 10.42FPAYY290 pKa = 9.71KK291 pKa = 9.97SWLGQQAEE299 pKa = 5.02FYY301 pKa = 9.88CTDD304 pKa = 3.05KK305 pKa = 10.79FRR307 pKa = 11.84KK308 pKa = 9.1KK309 pKa = 10.52KK310 pKa = 8.41YY311 pKa = 9.24IKK313 pKa = 9.54WGRR316 pKa = 11.84PCIWIMNEE324 pKa = 3.73DD325 pKa = 4.63PYY327 pKa = 10.22TQEE330 pKa = 5.17VDD332 pKa = 3.45IDD334 pKa = 3.78WLEE337 pKa = 4.06KK338 pKa = 10.45NCLIVHH344 pKa = 7.01IDD346 pKa = 3.45RR347 pKa = 11.84PLYY350 pKa = 8.64QTAA353 pKa = 3.5

Molecular weight: 41.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221LEA2|A0A221LEA2_9VIRU Capsid OS=Porcine feces-associated gemycircularvirus OX=2017712 PE=4 SV=1
MM1 pKa = 8.02AYY3 pKa = 10.14RR4 pKa = 11.84FRR6 pKa = 11.84RR7 pKa = 11.84FSTRR11 pKa = 11.84SRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84FTPRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84SSFRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84NYY29 pKa = 10.62RR30 pKa = 11.84MMRR33 pKa = 11.84RR34 pKa = 11.84PRR36 pKa = 11.84RR37 pKa = 11.84FSRR40 pKa = 11.84PRR42 pKa = 11.84TNVRR46 pKa = 11.84RR47 pKa = 11.84VRR49 pKa = 11.84NLASRR54 pKa = 11.84KK55 pKa = 9.61CRR57 pKa = 11.84DD58 pKa = 3.33NMLSNPIDD66 pKa = 3.78DD67 pKa = 4.48TGSPTTPGPVTMTGGNTYY85 pKa = 11.14AFIFSPSARR94 pKa = 11.84VAGNEE99 pKa = 3.61ISSGDD104 pKa = 3.56RR105 pKa = 11.84VNQPSSSQRR114 pKa = 11.84RR115 pKa = 11.84KK116 pKa = 9.89SKK118 pKa = 10.77CFVSGYY124 pKa = 10.48GEE126 pKa = 4.14TVEE129 pKa = 4.76IQTDD133 pKa = 3.94DD134 pKa = 3.29ASPWVWRR141 pKa = 11.84RR142 pKa = 11.84IVFSTIGLAVQFQPGLLYY160 pKa = 11.26NNDD163 pKa = 3.23DD164 pKa = 3.33TRR166 pKa = 11.84GYY168 pKa = 11.15EE169 pKa = 3.61RR170 pKa = 11.84TTFNLASGTAQADD183 pKa = 3.51QLQGIVYY190 pKa = 9.62RR191 pKa = 11.84YY192 pKa = 9.63LFQGSQDD199 pKa = 3.39IDD201 pKa = 3.3WANVMTANTAPRR213 pKa = 11.84HH214 pKa = 5.01VKK216 pKa = 9.97VYY218 pKa = 8.6PDD220 pKa = 3.47RR221 pKa = 11.84RR222 pKa = 11.84RR223 pKa = 11.84VIQSPNSEE231 pKa = 3.95GGTIRR236 pKa = 11.84ISKK239 pKa = 10.06HH240 pKa = 4.66YY241 pKa = 10.81DD242 pKa = 2.98NIRR245 pKa = 11.84RR246 pKa = 11.84SIIYY250 pKa = 10.02DD251 pKa = 4.07DD252 pKa = 4.12IEE254 pKa = 5.7AGSGAKK260 pKa = 8.39ATNAIASGTPYY271 pKa = 11.37GNMGDD276 pKa = 3.97LFVVDD281 pKa = 5.42FFSSASDD288 pKa = 3.96DD289 pKa = 3.79EE290 pKa = 4.86TTGATFQPHH299 pKa = 4.82GRR301 pKa = 11.84YY302 pKa = 8.68YY303 pKa = 8.44WHH305 pKa = 7.46EE306 pKa = 4.17GQAVV310 pKa = 3.08

Molecular weight: 35.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

663

310

353

331.5

38.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.335 ± 0.286

1.207 ± 0.366

7.089 ± 0.625

3.62 ± 0.677

6.033 ± 0.568

7.541 ± 0.131

2.564 ± 0.83

4.977 ± 0.12

3.469 ± 0.999

5.43 ± 1.646

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.508 ± 0.488

4.374 ± 0.723

4.525 ± 0.415

4.072 ± 0.079

11.463 ± 1.358

7.843 ± 1.195

6.033 ± 1.113

4.827 ± 0.638

2.262 ± 0.633

4.827 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski