Acidithiobacillus sp. SH

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales; Acidithiobacillaceae; Acidithiobacillus; unclassified Acidithiobacillus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I1DMJ9|A0A2I1DMJ9_9PROT Gly-zipper_Omp domain-containing protein OS=Acidithiobacillus sp. SH OX=187490 GN=B1757_06265 PE=4 SV=1
MM1 pKa = 6.85STATEE6 pKa = 4.19TMTNLDD12 pKa = 4.28SIPPVMTLTQNAADD26 pKa = 4.82KK27 pKa = 10.56ISALIEE33 pKa = 4.16EE34 pKa = 5.06EE35 pKa = 4.15GSEE38 pKa = 4.04DD39 pKa = 3.0LKK41 pKa = 11.4LRR43 pKa = 11.84VFVTGGGCSGFQYY56 pKa = 11.01GFTFDD61 pKa = 4.11EE62 pKa = 4.55NMNEE66 pKa = 3.96GDD68 pKa = 3.97TEE70 pKa = 4.19VHH72 pKa = 5.42QFGVSLLIDD81 pKa = 3.75PMSYY85 pKa = 10.31QYY87 pKa = 11.58LVGAEE92 pKa = 3.74IDD94 pKa = 3.65YY95 pKa = 11.51SEE97 pKa = 4.53GLEE100 pKa = 4.05GAQFVIKK107 pKa = 10.5NPNATTTCGCGSSFSAA123 pKa = 4.79

Molecular weight: 13.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I1DI10|A0A2I1DI10_9PROT Membrane protein insertase YidC OS=Acidithiobacillus sp. SH OX=187490 GN=yidC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTHH11 pKa = 7.03RR12 pKa = 11.84KK13 pKa = 7.23RR14 pKa = 11.84THH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29SGRR28 pKa = 11.84LVLKK32 pKa = 10.26RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.25GRR39 pKa = 11.84QRR41 pKa = 11.84LCPP44 pKa = 3.78

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2792

0

2792

849494

32

3220

304.3

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.662 ± 0.046

0.92 ± 0.017

4.961 ± 0.036

5.603 ± 0.053

3.645 ± 0.032

7.629 ± 0.059

2.711 ± 0.028

5.548 ± 0.039

3.64 ± 0.042

11.137 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.619 ± 0.025

3.118 ± 0.047

5.092 ± 0.035

4.863 ± 0.04

6.271 ± 0.054

5.859 ± 0.05

4.817 ± 0.047

6.662 ± 0.041

1.601 ± 0.023

2.643 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski