Micromonospora sp. HK10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5548 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2RH07|A0A0M2RH07_9ACTN MFS transporter OS=Micromonospora sp. HK10 OX=1538294 GN=LQ51_31065 PE=4 SV=1
MM1 pKa = 6.97TALAFTVSAPAQANDD16 pKa = 3.59HH17 pKa = 6.22EE18 pKa = 5.26NIPGEE23 pKa = 4.01PTITVPINDD32 pKa = 3.64SAAGGTYY39 pKa = 10.09NQDD42 pKa = 3.11FTRR45 pKa = 11.84VYY47 pKa = 11.41ANTTPDD53 pKa = 3.31GTPVYY58 pKa = 10.08IYY60 pKa = 10.52VPKK63 pKa = 9.73GTPLDD68 pKa = 3.74GTAPTGVASLDD79 pKa = 3.45PQFDD83 pKa = 4.21HH84 pKa = 7.16NPGDD88 pKa = 3.72EE89 pKa = 4.42FVPCATATAAQFTLTQAQITYY110 pKa = 10.67VGDD113 pKa = 3.35KK114 pKa = 10.18LANQIVAVDD123 pKa = 4.02EE124 pKa = 4.38EE125 pKa = 5.08HH126 pKa = 6.85FGPMDD131 pKa = 3.96AADD134 pKa = 4.22PADD137 pKa = 4.08PASDD141 pKa = 3.6SLVMLVYY148 pKa = 10.79NIQDD152 pKa = 3.32DD153 pKa = 4.43AYY155 pKa = 9.35YY156 pKa = 10.54DD157 pKa = 3.97CAVTSYY163 pKa = 9.73TAGYY167 pKa = 7.63FAPDD171 pKa = 4.87FIDD174 pKa = 4.12SIGMNVISVDD184 pKa = 3.38GLDD187 pKa = 3.07WANRR191 pKa = 11.84VGPNDD196 pKa = 4.05SPWRR200 pKa = 11.84DD201 pKa = 3.06ADD203 pKa = 3.81PANDD207 pKa = 3.72RR208 pKa = 11.84PEE210 pKa = 4.78LYY212 pKa = 9.95EE213 pKa = 3.8GTVAHH218 pKa = 6.3EE219 pKa = 4.42LEE221 pKa = 4.91HH222 pKa = 6.83LLHH225 pKa = 6.67NYY227 pKa = 9.83SDD229 pKa = 4.0PGEE232 pKa = 4.2LSWVDD237 pKa = 3.2EE238 pKa = 4.25GLADD242 pKa = 4.89FAIFLNGLDD251 pKa = 3.84AGGSHH256 pKa = 6.51LTNHH260 pKa = 4.98QVLYY264 pKa = 10.66EE265 pKa = 4.13EE266 pKa = 4.79TSLTRR271 pKa = 11.84WGGSLANYY279 pKa = 8.39GAAFTYY285 pKa = 9.16FQYY288 pKa = 10.98LWEE291 pKa = 4.13QAGGNGDD298 pKa = 4.05GTWTPDD304 pKa = 2.86KK305 pKa = 11.09QYY307 pKa = 11.18DD308 pKa = 3.87GKK310 pKa = 11.05AGDD313 pKa = 4.72LLIKK317 pKa = 9.82TIFEE321 pKa = 4.07EE322 pKa = 4.52QADD325 pKa = 3.96GMTGVQNAIDD335 pKa = 4.61DD336 pKa = 4.3FNAATGANLRR346 pKa = 11.84SAADD350 pKa = 3.93LFRR353 pKa = 11.84DD354 pKa = 3.46WSVAVYY360 pKa = 10.7LDD362 pKa = 5.32DD363 pKa = 4.81EE364 pKa = 5.26DD365 pKa = 5.57SPIYY369 pKa = 9.82DD370 pKa = 2.86IKK372 pKa = 11.21AFNFGDD378 pKa = 3.86PADD381 pKa = 4.44TSWTMDD387 pKa = 3.31IADD390 pKa = 3.65DD391 pKa = 4.46VYY393 pKa = 9.68WGGRR397 pKa = 11.84GKK399 pKa = 10.62YY400 pKa = 9.76HH401 pKa = 7.13GATPEE406 pKa = 4.11AKK408 pKa = 9.21WARR411 pKa = 11.84LKK413 pKa = 10.72NRR415 pKa = 11.84PDD417 pKa = 3.11TTAVPFGMSVEE428 pKa = 4.27RR429 pKa = 11.84FRR431 pKa = 11.84NPGPTVSVAFDD442 pKa = 3.9GDD444 pKa = 4.11DD445 pKa = 3.51EE446 pKa = 5.01TVVPPHH452 pKa = 6.56TGATHH457 pKa = 6.73WYY459 pKa = 9.47GGYY462 pKa = 10.23EE463 pKa = 4.08SQDD466 pKa = 3.59DD467 pKa = 4.51NILDD471 pKa = 3.87VTTSGAVTSLDD482 pKa = 2.99FWSWYY487 pKa = 10.04FIEE490 pKa = 4.87EE491 pKa = 4.05GWDD494 pKa = 3.46FGFVEE499 pKa = 4.83ALVGGNWVTVPLRR512 pKa = 11.84TDD514 pKa = 3.44DD515 pKa = 4.1GQVVTTNDD523 pKa = 3.8DD524 pKa = 3.28PHH526 pKa = 8.04GNNTEE531 pKa = 4.32GNGLTGTSGGEE542 pKa = 4.02YY543 pKa = 10.35FVDD546 pKa = 3.8DD547 pKa = 4.4PVYY550 pKa = 10.6QHH552 pKa = 6.59LTADD556 pKa = 4.52LPAGTTDD563 pKa = 2.73VRR565 pKa = 11.84FRR567 pKa = 11.84YY568 pKa = 8.57STDD571 pKa = 2.88AAYY574 pKa = 9.84MDD576 pKa = 4.32TGWFVDD582 pKa = 3.81DD583 pKa = 4.01VRR585 pKa = 11.84VNGADD590 pKa = 3.38AQLSSAEE597 pKa = 4.38GNWFATDD604 pKa = 4.12GAQDD608 pKa = 3.87NNWTVQVVSHH618 pKa = 7.17CDD620 pKa = 3.26LTPGVTSPGEE630 pKa = 4.28TTDD633 pKa = 4.23GAGNWVYY640 pKa = 10.96RR641 pKa = 11.84STGDD645 pKa = 3.26QFTMSGLRR653 pKa = 11.84TKK655 pKa = 10.3CANGNQDD662 pKa = 4.03DD663 pKa = 4.16FAVIINNLPTGDD675 pKa = 4.23LTYY678 pKa = 11.12LDD680 pKa = 3.93ADD682 pKa = 3.59YY683 pKa = 10.57TFRR686 pKa = 11.84VKK688 pKa = 9.97NTGNAKK694 pKa = 10.2

Molecular weight: 75.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2RXK1|A0A0M2RXK1_9ACTN Alanine--tRNA ligase OS=Micromonospora sp. HK10 OX=1538294 GN=alaS PE=3 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84RR3 pKa = 11.84TTTRR7 pKa = 11.84PRR9 pKa = 11.84IRR11 pKa = 11.84RR12 pKa = 11.84LRR14 pKa = 11.84RR15 pKa = 11.84PAGRR19 pKa = 11.84PRR21 pKa = 11.84IRR23 pKa = 11.84RR24 pKa = 11.84LRR26 pKa = 11.84RR27 pKa = 11.84TPTRR31 pKa = 11.84PRR33 pKa = 11.84IRR35 pKa = 11.84RR36 pKa = 11.84LRR38 pKa = 11.84RR39 pKa = 11.84TPTRR43 pKa = 11.84PRR45 pKa = 11.84IRR47 pKa = 11.84RR48 pKa = 11.84LRR50 pKa = 11.84RR51 pKa = 11.84AGRR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84VRR58 pKa = 11.84RR59 pKa = 11.84AGRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84PARR67 pKa = 11.84RR68 pKa = 11.84GRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84GGRR75 pKa = 11.84PAGRR79 pKa = 11.84RR80 pKa = 11.84VRR82 pKa = 11.84AGWAGGGVGHH92 pKa = 7.09PRR94 pKa = 11.84DD95 pKa = 3.84PRR97 pKa = 11.84AGDD100 pKa = 3.28RR101 pKa = 11.84GAAAPPAGRR110 pKa = 11.84RR111 pKa = 11.84HH112 pKa = 6.07HH113 pKa = 6.94PGAPVRR119 pKa = 11.84CRR121 pKa = 11.84SRR123 pKa = 11.84RR124 pKa = 11.84RR125 pKa = 11.84GGPRR129 pKa = 11.84PAGRR133 pKa = 11.84RR134 pKa = 11.84TPRR137 pKa = 11.84PPPPPCATRR146 pKa = 11.84CPTPAPDD153 pKa = 3.25GG154 pKa = 3.94

Molecular weight: 17.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5548

0

5548

1776529

29

3736

320.2

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.421 ± 0.052

0.72 ± 0.01

6.047 ± 0.026

5.064 ± 0.031

2.63 ± 0.02

9.5 ± 0.033

2.127 ± 0.019

3.086 ± 0.022

1.609 ± 0.024

10.77 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.544 ± 0.011

1.724 ± 0.019

6.43 ± 0.031

2.696 ± 0.019

8.583 ± 0.036

4.407 ± 0.023

5.974 ± 0.031

8.99 ± 0.031

1.607 ± 0.015

2.071 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski