Chlamydotis macqueenii (Macqueen s bustard)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria;

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8277 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091KLE7|A0A091KLE7_9GRUI Neuroblast differentiation-associated protein AHNAK (Fragment) OS=Chlamydotis macqueenii OX=187382 GN=N324_04926 PE=4 SV=1
QQ1 pKa = 7.23CRR3 pKa = 11.84YY4 pKa = 9.78SPCFPGVRR12 pKa = 11.84CVNTAPGFRR21 pKa = 11.84CEE23 pKa = 4.17TCPPGYY29 pKa = 9.16TGQTVQGVGLSYY41 pKa = 11.12AKK43 pKa = 10.53SNKK46 pKa = 7.95QVCLDD51 pKa = 3.73IDD53 pKa = 3.67EE54 pKa = 4.91CQNGGLGLCVPNSHH68 pKa = 7.46CINTLGSYY76 pKa = 10.2RR77 pKa = 11.84CGQCKK82 pKa = 9.57PGYY85 pKa = 9.17TGDD88 pKa = 3.61QARR91 pKa = 11.84GCQAEE96 pKa = 4.14RR97 pKa = 11.84SCRR100 pKa = 11.84NRR102 pKa = 11.84ALNPCSVHH110 pKa = 5.91ARR112 pKa = 11.84CIEE115 pKa = 4.02EE116 pKa = 3.8KK117 pKa = 10.27RR118 pKa = 11.84GEE120 pKa = 4.27VTCICGIGWAGDD132 pKa = 3.82GYY134 pKa = 10.69ICGKK138 pKa = 10.5DD139 pKa = 3.18IDD141 pKa = 4.02IDD143 pKa = 4.15GYY145 pKa = 10.37PNDD148 pKa = 4.44EE149 pKa = 4.52LSCSAEE155 pKa = 3.77NCRR158 pKa = 11.84KK159 pKa = 10.08DD160 pKa = 3.11NCRR163 pKa = 11.84FVPNSGQEE171 pKa = 4.02DD172 pKa = 4.12ADD174 pKa = 3.79GDD176 pKa = 4.61GIGDD180 pKa = 4.07ACDD183 pKa = 3.53EE184 pKa = 4.74DD185 pKa = 5.71ADD187 pKa = 4.46GDD189 pKa = 4.49GIPNEE194 pKa = 4.01QDD196 pKa = 3.11NCVLAPNVNQRR207 pKa = 11.84NVDD210 pKa = 3.19QDD212 pKa = 3.47IFGDD216 pKa = 3.6ACDD219 pKa = 3.4NCRR222 pKa = 11.84NILNNDD228 pKa = 3.09QRR230 pKa = 11.84DD231 pKa = 3.44TDD233 pKa = 3.87GDD235 pKa = 4.16GKK237 pKa = 11.42GDD239 pKa = 3.74ACDD242 pKa = 5.17DD243 pKa = 4.26DD244 pKa = 4.15MDD246 pKa = 6.26GDD248 pKa = 4.5GIKK251 pKa = 10.77NLLDD255 pKa = 3.41NCQRR259 pKa = 11.84IPNQDD264 pKa = 3.43QEE266 pKa = 5.62DD267 pKa = 3.92KK268 pKa = 11.46DD269 pKa = 3.9NDD271 pKa = 4.31GVGDD275 pKa = 4.14ACDD278 pKa = 3.51SCPTISNPNQSDD290 pKa = 3.32VDD292 pKa = 3.63NDD294 pKa = 4.07LVGDD298 pKa = 4.11SCDD301 pKa = 3.63TNQDD305 pKa = 3.04SDD307 pKa = 4.07GDD309 pKa = 4.03GHH311 pKa = 7.17QDD313 pKa = 3.22STDD316 pKa = 3.33NCPTIINSSQLDD328 pKa = 3.61TDD330 pKa = 3.69KK331 pKa = 11.62DD332 pKa = 4.04GLGDD336 pKa = 3.77EE337 pKa = 5.06CDD339 pKa = 4.04EE340 pKa = 6.11DD341 pKa = 5.22DD342 pKa = 5.9DD343 pKa = 5.67NDD345 pKa = 5.36GILDD349 pKa = 4.79LLPPGPDD356 pKa = 2.92NCRR359 pKa = 11.84LVPNPGQEE367 pKa = 4.18DD368 pKa = 4.25DD369 pKa = 4.42NGDD372 pKa = 3.69GVGDD376 pKa = 3.48ICEE379 pKa = 4.29SDD381 pKa = 3.45FDD383 pKa = 3.9QDD385 pKa = 3.89TVIDD389 pKa = 4.23QIDD392 pKa = 3.82VCPEE396 pKa = 3.54NAEE399 pKa = 3.78ITLTDD404 pKa = 3.83FRR406 pKa = 11.84AYY408 pKa = 8.19QTVVLDD414 pKa = 4.22PEE416 pKa = 5.32GDD418 pKa = 3.65AQIDD422 pKa = 4.13PNWVVLNQGMEE433 pKa = 4.11IVQTMNSDD441 pKa = 3.19PGLAVGYY448 pKa = 7.31TAFNGVDD455 pKa = 3.99FEE457 pKa = 4.97GTFHH461 pKa = 6.81VNTVTDD467 pKa = 3.57DD468 pKa = 4.08DD469 pKa = 4.35YY470 pKa = 12.0AGFIFGYY477 pKa = 9.58QDD479 pKa = 2.83SSSFYY484 pKa = 10.47VVMWKK489 pKa = 7.78QTEE492 pKa = 3.86QTYY495 pKa = 8.13WQATPFRR502 pKa = 11.84AVAEE506 pKa = 4.17PGIQLKK512 pKa = 10.07AVKK515 pKa = 10.09SKK517 pKa = 9.55TGPGEE522 pKa = 3.82HH523 pKa = 6.52LRR525 pKa = 11.84NSLWHH530 pKa = 6.51TGDD533 pKa = 3.06TSDD536 pKa = 5.19QVRR539 pKa = 11.84LLWKK543 pKa = 10.3DD544 pKa = 3.22PRR546 pKa = 11.84NVGWKK551 pKa = 10.39DD552 pKa = 3.18KK553 pKa = 11.21VSYY556 pKa = 10.33RR557 pKa = 11.84WFLQHH562 pKa = 6.9RR563 pKa = 11.84PQIGYY568 pKa = 9.07IRR570 pKa = 11.84ARR572 pKa = 11.84FYY574 pKa = 11.09EE575 pKa = 4.54GSDD578 pKa = 3.39LVADD582 pKa = 3.84SGVTIDD588 pKa = 3.09TTMRR592 pKa = 11.84GGRR595 pKa = 11.84LGVFCFSQEE604 pKa = 3.94NIIWSNLKK612 pKa = 9.68YY613 pKa = 10.31RR614 pKa = 11.84CNDD617 pKa = 4.18TIPEE621 pKa = 4.31DD622 pKa = 3.77FQEE625 pKa = 4.53FQAQQFGVGDD635 pKa = 3.49II636 pKa = 4.15

Molecular weight: 69.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091KCL2|A0A091KCL2_9GRUI Stanniocalcin-1 (Fragment) OS=Chlamydotis macqueenii OX=187382 GN=N324_02285 PE=3 SV=1
DDD2 pKa = 3.69EE3 pKa = 5.64DD4 pKa = 4.23LVRR7 pKa = 11.84GLSVLCNLANQLYYY21 pKa = 8.69YY22 pKa = 10.2CEEE25 pKa = 4.04HH26 pKa = 6.39AWAADDD32 pKa = 3.38GIIRR36 pKa = 11.84VSSQKKK42 pKa = 8.66WTLSTALWAFSLLLGLLRR60 pKa = 11.84SLRR63 pKa = 11.84ILFRR67 pKa = 11.84LRR69 pKa = 11.84RR70 pKa = 11.84KKK72 pKa = 9.14QQHHH76 pKa = 5.76KK77 pKa = 9.88ASPPLSRR84 pKa = 11.84KKK86 pKa = 5.44EE87 pKa = 3.9KKK89 pKa = 10.51RR90 pKa = 11.84VQAEEE95 pKa = 4.26LSILTDDD102 pKa = 3.62ADDD105 pKa = 3.9ANAIHHH111 pKa = 7.0LPAGFLWAGRR121 pKa = 11.84LPAWSVGLLGTISSLIGIYYY141 pKa = 9.57AARR144 pKa = 11.84GRR146 pKa = 11.84NS

Molecular weight: 16.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8277

0

8277

3590270

33

5242

433.8

48.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.38 ± 0.022

2.232 ± 0.023

5.043 ± 0.018

7.232 ± 0.032

3.942 ± 0.02

5.767 ± 0.035

2.523 ± 0.014

5.046 ± 0.019

6.495 ± 0.029

9.739 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.011

4.185 ± 0.017

5.219 ± 0.034

4.692 ± 0.025

5.127 ± 0.019

8.228 ± 0.036

5.407 ± 0.021

6.293 ± 0.021

1.183 ± 0.01

3.006 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski