Adelaide River virus (ARV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ephemerovirus; Adelaide River ephemerovirus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4HH67|K4HH67_ARV Beta protein OS=Adelaide River virus OX=31612 GN=beta PE=4 SV=1
MM1 pKa = 7.6EE2 pKa = 5.49KK3 pKa = 10.51FNPSKK8 pKa = 11.13VLGEE12 pKa = 4.11YY13 pKa = 9.21DD14 pKa = 3.57TDD16 pKa = 4.18KK17 pKa = 11.38LIASINEE24 pKa = 4.43TNWNLDD30 pKa = 3.41DD31 pKa = 5.58DD32 pKa = 5.12PEE34 pKa = 4.47FSAPQEE40 pKa = 4.31SIEE43 pKa = 4.19PSNSIDD49 pKa = 3.37NYY51 pKa = 9.63RR52 pKa = 11.84DD53 pKa = 3.5EE54 pKa = 4.06IVKK57 pKa = 10.36IGVCLSSNNQDD68 pKa = 3.35YY69 pKa = 11.6SFLDD73 pKa = 3.43NQKK76 pKa = 9.89EE77 pKa = 4.06KK78 pKa = 10.96EE79 pKa = 4.04IFLEE83 pKa = 4.67EE84 pKa = 4.0EE85 pKa = 3.92NDD87 pKa = 3.54WEE89 pKa = 4.85VEE91 pKa = 4.29FTKK94 pKa = 10.74KK95 pKa = 10.84LEE97 pKa = 4.07DD98 pKa = 3.46HH99 pKa = 6.44NVPNEE104 pKa = 4.13SEE106 pKa = 4.89LEE108 pKa = 4.06VQINIEE114 pKa = 4.06DD115 pKa = 5.14LIALLHH121 pKa = 5.76FLNVEE126 pKa = 3.71IDD128 pKa = 3.43QYY130 pKa = 11.91NILPLSLQNKK140 pKa = 9.52VILQRR145 pKa = 11.84KK146 pKa = 9.12KK147 pKa = 10.45EE148 pKa = 4.06GKK150 pKa = 9.84SDD152 pKa = 3.49DD153 pKa = 4.06KK154 pKa = 11.25KK155 pKa = 10.39RR156 pKa = 11.84QNGKK160 pKa = 9.83GDD162 pKa = 3.66KK163 pKa = 10.24KK164 pKa = 10.94SCPDD168 pKa = 4.58SKK170 pKa = 11.17NQSNNCQTMNAEE182 pKa = 4.69TNGSTNLGADD192 pKa = 3.65RR193 pKa = 11.84GPNQTEE199 pKa = 4.57DD200 pKa = 3.44NSSMLEE206 pKa = 3.96NDD208 pKa = 3.4SEE210 pKa = 5.74AILDD214 pKa = 3.76SDD216 pKa = 4.4GGEE219 pKa = 3.79LFQFIIKK226 pKa = 9.65TMDD229 pKa = 2.66QGIRR233 pKa = 11.84VKK235 pKa = 10.8KK236 pKa = 9.93KK237 pKa = 9.66FSGKK241 pKa = 8.99KK242 pKa = 9.31VKK244 pKa = 8.69ITRR247 pKa = 11.84EE248 pKa = 4.08NIGLDD253 pKa = 3.08YY254 pKa = 11.02HH255 pKa = 6.95VINGHH260 pKa = 4.91IHH262 pKa = 6.03GKK264 pKa = 7.33SAKK267 pKa = 10.29DD268 pKa = 3.52SIDD271 pKa = 3.28LKK273 pKa = 11.19KK274 pKa = 10.92LMRR277 pKa = 11.84EE278 pKa = 4.37LIKK281 pKa = 10.61KK282 pKa = 7.77GRR284 pKa = 11.84KK285 pKa = 8.52YY286 pKa = 10.86KK287 pKa = 10.57DD288 pKa = 3.21YY289 pKa = 11.42SNQLDD294 pKa = 3.51LSEE297 pKa = 5.02IEE299 pKa = 4.29FF300 pKa = 4.22

Molecular weight: 34.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4HJF7|K4HJF7_ARV Isoform of K4HKI5 Alpha 2 protein OS=Adelaide River virus OX=31612 GN=alpha PE=4 SV=1
MM1 pKa = 7.9ADD3 pKa = 3.31HH4 pKa = 7.08ARR6 pKa = 11.84LISLPSSVQDD16 pKa = 3.38FFNQISSIFKK26 pKa = 10.25SIEE29 pKa = 3.77NFYY32 pKa = 11.17LLYY35 pKa = 9.07KK36 pKa = 9.9TRR38 pKa = 11.84LKK40 pKa = 10.62IFLICTIALIVIVLVAKK57 pKa = 10.32IIKK60 pKa = 8.21YY61 pKa = 7.96TLAFHH66 pKa = 6.95ACIKK70 pKa = 10.08IYY72 pKa = 10.27CKK74 pKa = 10.31PLIKK78 pKa = 8.49TTNCLLKK85 pKa = 10.45LGKK88 pKa = 9.8RR89 pKa = 11.84KK90 pKa = 9.46RR91 pKa = 11.84RR92 pKa = 11.84KK93 pKa = 9.4KK94 pKa = 9.47KK95 pKa = 10.32RR96 pKa = 11.84KK97 pKa = 8.98GKK99 pKa = 9.7SIRR102 pKa = 11.84LKK104 pKa = 10.41EE105 pKa = 3.98LSSS108 pKa = 3.53

Molecular weight: 12.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

1

9

4719

100

2144

524.3

60.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.437 ± 0.265

1.759 ± 0.303

5.912 ± 0.421

6.887 ± 0.655

4.153 ± 0.203

5.043 ± 0.226

2.352 ± 0.252

9.176 ± 0.441

8.646 ± 0.604

9.833 ± 0.651

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.67 ± 0.472

7.438 ± 0.482

3.285 ± 0.166

2.882 ± 0.256

4.471 ± 0.269

7.819 ± 0.544

4.641 ± 0.574

4.62 ± 0.45

1.441 ± 0.257

4.535 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski