Sinimarinibacterium flocculans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Nevskiales; Sinobacteraceae; Sinimarinibacterium

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4018 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A318EDB5|A0A318EDB5_9GAMM Cytochrome P450 OS=Sinimarinibacterium flocculans OX=985250 GN=C8D93_102340 PE=3 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84TLANLGVVLALAAPCSAFAGHH23 pKa = 6.13QSNLDD28 pKa = 3.29GYY30 pKa = 9.56YY31 pKa = 9.69IPQSEE36 pKa = 4.5FSFEE40 pKa = 4.28PEE42 pKa = 3.4TALPFDD48 pKa = 4.38FDD50 pKa = 4.71EE51 pKa = 5.28NGDD54 pKa = 3.73GYY56 pKa = 10.88GIKK59 pKa = 10.42AQFQVSSVFFIHH71 pKa = 7.33GEE73 pKa = 4.04YY74 pKa = 10.05QAIDD78 pKa = 3.69YY79 pKa = 11.41GEE81 pKa = 5.21DD82 pKa = 3.74DD83 pKa = 3.86DD84 pKa = 5.4TGVLKK89 pKa = 10.74DD90 pKa = 4.23DD91 pKa = 4.58LNTYY95 pKa = 10.27RR96 pKa = 11.84IGGGFFFSDD105 pKa = 3.46SQPFFVKK112 pKa = 10.7GEE114 pKa = 4.18YY115 pKa = 9.74IGVDD119 pKa = 3.73LGDD122 pKa = 4.13SDD124 pKa = 6.29DD125 pKa = 4.17GDD127 pKa = 3.91EE128 pKa = 5.91EE129 pKa = 4.85IDD131 pKa = 3.57EE132 pKa = 5.1DD133 pKa = 4.7YY134 pKa = 11.37NGYY137 pKa = 9.51GVHH140 pKa = 6.55IGALGHH146 pKa = 6.9VGDD149 pKa = 5.0ALNLTASIGYY159 pKa = 9.71VDD161 pKa = 5.14LDD163 pKa = 3.55GTAGIEE169 pKa = 4.02FLVGVGFQLTPLIGVFADD187 pKa = 3.58YY188 pKa = 11.1RR189 pKa = 11.84NTDD192 pKa = 3.39LEE194 pKa = 5.13LDD196 pKa = 4.03DD197 pKa = 5.46GGGDD201 pKa = 3.76FKK203 pKa = 11.21LTDD206 pKa = 3.01VRR208 pKa = 11.84TGVRR212 pKa = 11.84FVFF215 pKa = 4.26

Molecular weight:
23.25 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A318E3N4|A0A318E3N4_9GAMM RadC-like JAB domain-containing protein (Fragment) OS=Sinimarinibacterium flocculans OX=985250 GN=C8D93_1231 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 9.58QPKK9 pKa = 9.34KK10 pKa = 9.24LRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 7.83RR15 pKa = 11.84THH17 pKa = 5.96GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.34GGRR29 pKa = 11.84LVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.93GRR40 pKa = 11.84KK41 pKa = 8.79RR42 pKa = 11.84LAPP45 pKa = 3.99

Molecular weight:
5.35 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4018

0

4018

1348336

27

2842

335.6

36.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.784 ± 0.052

0.972 ± 0.013

6.004 ± 0.031

5.607 ± 0.031

3.446 ± 0.02

8.503 ± 0.035

2.186 ± 0.02

4.304 ± 0.024

2.505 ± 0.031

10.759 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.138 ± 0.019

2.338 ± 0.023

5.449 ± 0.034

3.605 ± 0.02

8.09 ± 0.049

4.978 ± 0.027

4.99 ± 0.029

7.437 ± 0.033

1.484 ± 0.017

2.419 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski