Clostridium phage CPS2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Guelinviridae; Brucesealvirus; Clostridium virus CPS2

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A343X838|A0A343X838_9CAUD Major head protein OS=Clostridium phage CPS2 OX=2175605 GN=CPS2_7 PE=4 SV=1
MM1 pKa = 7.48TDD3 pKa = 3.41EE4 pKa = 5.54EE5 pKa = 4.28IQALQQSVIEE15 pKa = 4.13LQEE18 pKa = 3.82KK19 pKa = 8.56QKK21 pKa = 11.34NYY23 pKa = 10.61DD24 pKa = 3.53LEE26 pKa = 4.28KK27 pKa = 10.69QAWEE31 pKa = 4.25TEE33 pKa = 4.16KK34 pKa = 10.62ATLSQQVEE42 pKa = 4.45TLKK45 pKa = 10.46ATADD49 pKa = 3.53TYY51 pKa = 11.92KK52 pKa = 11.3DD53 pKa = 3.49MNAKK57 pKa = 10.41LSLNLATQMTSTSVAGMPGKK77 pKa = 10.29KK78 pKa = 9.46EE79 pKa = 4.02EE80 pKa = 4.07QTEE83 pKa = 4.23PVDD86 pKa = 3.6NTPTLDD92 pKa = 5.7DD93 pKa = 5.31IINDD97 pKa = 3.94LL98 pKa = 4.2

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A343X845|A0A343X845_9CAUD Uncharacterized protein OS=Clostridium phage CPS2 OX=2175605 GN=CPS2_14 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.86HH3 pKa = 6.33INDD6 pKa = 2.96IAFWVRR12 pKa = 11.84TLSEE16 pKa = 4.09GTPKK20 pKa = 10.63GFYY23 pKa = 10.14QIGRR27 pKa = 11.84GVGTFF32 pKa = 3.21

Molecular weight: 3.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

5587

29

727

223.5

25.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.795 ± 0.364

1.682 ± 0.251

6.175 ± 0.196

7.482 ± 0.536

4.528 ± 0.331

7.213 ± 0.578

1.682 ± 0.232

7.875 ± 0.335

9.057 ± 0.703

7.213 ± 0.41

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.043 ± 0.305

8.037 ± 0.493

2.524 ± 0.226

2.792 ± 0.267

3.598 ± 0.286

5.298 ± 0.433

5.942 ± 0.371

5.549 ± 0.354

1.128 ± 0.144

5.388 ± 0.416
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski