Cohnella sp. G13

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Cohnella

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A559J506|A0A559J506_9BACL Ankyrin repeat domain-containing protein OS=Cohnella sp. G13 OX=1289167 GN=FPZ45_24610 PE=4 SV=1
MM1 pKa = 7.58GRR3 pKa = 11.84VTWLFTDD10 pKa = 4.75GAIDD14 pKa = 3.71EE15 pKa = 4.34QVIIAVEE22 pKa = 3.78NSFGVRR28 pKa = 11.84FPDD31 pKa = 5.16DD32 pKa = 3.35YY33 pKa = 11.59RR34 pKa = 11.84EE35 pKa = 4.37CIKK38 pKa = 10.75KK39 pKa = 10.55NNGGYY44 pKa = 9.49PEE46 pKa = 4.36PNIYY50 pKa = 10.23DD51 pKa = 3.55INDD54 pKa = 3.88DD55 pKa = 3.4EE56 pKa = 6.07GGAVFDD62 pKa = 4.92CLLSFTSKK70 pKa = 10.6DD71 pKa = 3.28ANIEE75 pKa = 3.96VVYY78 pKa = 10.22EE79 pKa = 4.04LTSLPEE85 pKa = 4.31GIIPFARR92 pKa = 11.84DD93 pKa = 3.2PFGGLICFNYY103 pKa = 10.1CDD105 pKa = 4.78AKK107 pKa = 11.0DD108 pKa = 3.57SPTVVFYY115 pKa = 11.04DD116 pKa = 3.62EE117 pKa = 4.89EE118 pKa = 4.68SVNEE122 pKa = 4.13DD123 pKa = 4.08KK124 pKa = 11.02IKK126 pKa = 10.75LICDD130 pKa = 3.67SFSEE134 pKa = 4.9LIDD137 pKa = 3.72RR138 pKa = 11.84LYY140 pKa = 11.46SDD142 pKa = 4.07

Molecular weight: 16.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A559JKG7|A0A559JKG7_9BACL VOC domain-containing protein OS=Cohnella sp. G13 OX=1289167 GN=FPZ45_10025 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.87PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.57KK14 pKa = 8.17VHH16 pKa = 5.5GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 10.35NGRR28 pKa = 11.84KK29 pKa = 8.13VLKK32 pKa = 10.34ARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.21GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4843

0

4843

1556618

23

2441

321.4

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.5 ± 0.043

0.74 ± 0.01

5.273 ± 0.028

6.701 ± 0.04

4.13 ± 0.026

7.532 ± 0.036

1.945 ± 0.017

6.693 ± 0.032

5.279 ± 0.032

10.044 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.708 ± 0.018

3.862 ± 0.029

4.08 ± 0.023

3.48 ± 0.02

5.531 ± 0.038

6.419 ± 0.033

5.244 ± 0.03

7.142 ± 0.031

1.314 ± 0.016

3.384 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski