Brochothrix phage A9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Brochothrix virus A9

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 199 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9J0H2|D9J0H2_9CAUD Gp25 OS=Brochothrix phage A9 OX=857312 PE=4 SV=1
MM1 pKa = 8.21IIEE4 pKa = 4.37ITNSYY9 pKa = 10.17HH10 pKa = 5.02GTTEE14 pKa = 3.67IVEE17 pKa = 4.29DD18 pKa = 4.23EE19 pKa = 4.29SLEE22 pKa = 4.2VAKK25 pKa = 10.72NKK27 pKa = 10.12HH28 pKa = 5.07ISGLEE33 pKa = 3.85VTAGNLAEE41 pKa = 5.27NEE43 pKa = 4.44VEE45 pKa = 5.59DD46 pKa = 5.15DD47 pKa = 3.23WADD50 pKa = 3.46YY51 pKa = 10.01YY52 pKa = 11.07ATYY55 pKa = 10.76EE56 pKa = 4.07EE57 pKa = 5.35AEE59 pKa = 4.06QATYY63 pKa = 11.44DD64 pKa = 5.46DD65 pKa = 4.98ILNQLKK71 pKa = 10.7SEE73 pKa = 4.25VAYY76 pKa = 9.74KK77 pKa = 10.69VIEE80 pKa = 3.9QPMTIRR86 pKa = 11.84EE87 pKa = 4.11VMASVTEE94 pKa = 4.26YY95 pKa = 10.92KK96 pKa = 10.84DD97 pKa = 3.18EE98 pKa = 4.56ADD100 pKa = 3.74YY101 pKa = 10.4TKK103 pKa = 10.82HH104 pKa = 6.03EE105 pKa = 4.26NEE107 pKa = 4.13TLEE110 pKa = 4.33EE111 pKa = 4.01LLEE114 pKa = 4.42SDD116 pKa = 4.05FTHH119 pKa = 7.91DD120 pKa = 3.18ITPRR124 pKa = 11.84TYY126 pKa = 11.12NGASDD131 pKa = 3.21IAHH134 pKa = 6.1FHH136 pKa = 6.47EE137 pKa = 5.03LTPEE141 pKa = 4.44TIYY144 pKa = 11.23NALQRR149 pKa = 11.84DD150 pKa = 3.74MKK152 pKa = 10.46EE153 pKa = 4.0TDD155 pKa = 3.63FHH157 pKa = 8.01NDD159 pKa = 2.27IVEE162 pKa = 4.18YY163 pKa = 10.9DD164 pKa = 4.47DD165 pKa = 5.81DD166 pKa = 4.35LQGLSEE172 pKa = 4.79HH173 pKa = 6.72EE174 pKa = 4.55LLIQYY179 pKa = 9.24IEE181 pKa = 4.19CYY183 pKa = 10.39YY184 pKa = 10.8RR185 pKa = 11.84VFTLNGHH192 pKa = 7.16LYY194 pKa = 8.27ITNKK198 pKa = 10.16

Molecular weight: 23.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9J0K8|D9J0K8_9CAUD Gp61 OS=Brochothrix phage A9 OX=857312 PE=4 SV=1
MM1 pKa = 7.64SYY3 pKa = 11.09YY4 pKa = 10.08IGLLFKK10 pKa = 10.65ALNWATCTITTLSQTGLVSFSAKK33 pKa = 10.02RR34 pKa = 11.84LVGLPSATLAPTNAVKK50 pKa = 10.29AYY52 pKa = 8.83PSQRR56 pKa = 11.84CLLYY60 pKa = 10.47AKK62 pKa = 9.93RR63 pKa = 11.84VMCIILGTVHH73 pKa = 6.6VPFTSTGVFSTSTLASLDD91 pKa = 3.3LRR93 pKa = 11.84KK94 pKa = 9.8PFLEE98 pKa = 5.29AIATRR103 pKa = 11.84LDD105 pKa = 3.06MRR107 pKa = 11.84TYY109 pKa = 10.42ATVPLLAVLKK119 pKa = 8.37QFCTRR124 pKa = 11.84FLRR127 pKa = 11.84LPSKK131 pKa = 10.52DD132 pKa = 3.76LNGLLLYY139 pKa = 10.17II140 pKa = 4.99

Molecular weight: 15.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

199

0

199

38591

25

1191

193.9

21.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.509 ± 0.179

0.749 ± 0.067

6.009 ± 0.168

7.294 ± 0.289

3.475 ± 0.139

6.266 ± 0.258

1.969 ± 0.105

6.253 ± 0.149

6.789 ± 0.181

8.73 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.088

4.97 ± 0.159

3.503 ± 0.13

3.296 ± 0.184

4.395 ± 0.133

6.717 ± 0.196

7.292 ± 0.209

7.787 ± 0.225

0.935 ± 0.054

4.548 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski