Sheep faeces associated smacovirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; Porprismacovirus sheas1

Average proteome isoelectric point is 5.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160HWH4|A0A160HWH4_9VIRU Putative capsid protein OS=Sheep faeces associated smacovirus 1 OX=1843756 PE=4 SV=1
MM1 pKa = 7.2ATNFVKK7 pKa = 10.58CSYY10 pKa = 11.07KK11 pKa = 10.57EE12 pKa = 4.01IIDD15 pKa = 5.19LNTEE19 pKa = 4.29SNKK22 pKa = 8.07VTAIGIHH29 pKa = 6.07TPTGDD34 pKa = 3.69TPRR37 pKa = 11.84KK38 pKa = 8.03MFPGFFKK45 pKa = 10.47QYY47 pKa = 10.24RR48 pKa = 11.84KK49 pKa = 10.15YY50 pKa = 9.94KK51 pKa = 10.19YY52 pKa = 9.94SGASLAFYY60 pKa = 9.82PIAKK64 pKa = 10.0LPADD68 pKa = 4.41PLQVSYY74 pKa = 11.27DD75 pKa = 3.49AGEE78 pKa = 4.23PGIDD82 pKa = 4.05PRR84 pKa = 11.84DD85 pKa = 3.48LANPILVHH93 pKa = 6.23GCHH96 pKa = 6.42GEE98 pKa = 4.37DD99 pKa = 3.86MGQILNKK106 pKa = 10.53LYY108 pKa = 10.93GDD110 pKa = 3.79NNGISDD116 pKa = 4.38SLNLIEE122 pKa = 5.34GGSNIPVVGTDD133 pKa = 2.91PWYY136 pKa = 10.35DD137 pKa = 3.28LMEE140 pKa = 4.52RR141 pKa = 11.84LYY143 pKa = 11.27YY144 pKa = 10.24KK145 pKa = 10.84ALTDD149 pKa = 3.98RR150 pKa = 11.84TWKK153 pKa = 9.48QAGVQRR159 pKa = 11.84GFRR162 pKa = 11.84KK163 pKa = 10.31GGLRR167 pKa = 11.84PLLYY171 pKa = 10.35TLGTNHH177 pKa = 6.7QIMPSSQLSGMVLGDD192 pKa = 3.7DD193 pKa = 4.92GVPKK197 pKa = 10.12MLGGLPTTEE206 pKa = 4.06YY207 pKa = 10.76EE208 pKa = 3.92IDD210 pKa = 3.98EE211 pKa = 4.44EE212 pKa = 5.31DD213 pKa = 5.52EE214 pKa = 4.72PDD216 pKa = 3.81LLNNAVNKK224 pKa = 10.75SNIQFFTPRR233 pKa = 11.84LTRR236 pKa = 11.84LGWMDD241 pKa = 3.35TRR243 pKa = 11.84NVITTNTSQEE253 pKa = 4.48IPDD256 pKa = 3.56SWNADD261 pKa = 3.36TLEE264 pKa = 4.07TLFAQGMVDD273 pKa = 3.37QINWAEE279 pKa = 4.07LPNIYY284 pKa = 9.69MLMILLPPAYY294 pKa = 8.17KK295 pKa = 9.34TEE297 pKa = 3.76MYY299 pKa = 9.86FRR301 pKa = 11.84VVITHH306 pKa = 5.49YY307 pKa = 10.81FKK309 pKa = 11.05FKK311 pKa = 10.0GFRR314 pKa = 11.84GISMMPEE321 pKa = 3.73QTGVPSLVTQEE332 pKa = 4.16WNGSGPEE339 pKa = 4.05TKK341 pKa = 10.16TFDD344 pKa = 4.87DD345 pKa = 4.93LPIDD349 pKa = 4.18PPEE352 pKa = 4.73GGNPRR357 pKa = 11.84AA358 pKa = 4.08

Molecular weight: 40.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160HWH4|A0A160HWH4_9VIRU Putative capsid protein OS=Sheep faeces associated smacovirus 1 OX=1843756 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.09KK3 pKa = 10.28YY4 pKa = 10.52VLTVPRR10 pKa = 11.84EE11 pKa = 3.65ADD13 pKa = 3.27LRR15 pKa = 11.84PICRR19 pKa = 11.84MLSDD23 pKa = 3.59AKK25 pKa = 10.41KK26 pKa = 7.15WTIGFEE32 pKa = 4.2VGEE35 pKa = 4.28HH36 pKa = 6.35GFRR39 pKa = 11.84HH40 pKa = 4.55YY41 pKa = 10.59QIRR44 pKa = 11.84LVSSDD49 pKa = 2.98HH50 pKa = 7.77DD51 pKa = 3.66FFEE54 pKa = 4.23WCKK57 pKa = 10.95AFLPTAHH64 pKa = 6.9IEE66 pKa = 4.17EE67 pKa = 4.49ATEE70 pKa = 3.76EE71 pKa = 4.12RR72 pKa = 11.84DD73 pKa = 3.46DD74 pKa = 4.1YY75 pKa = 11.41EE76 pKa = 4.52RR77 pKa = 11.84KK78 pKa = 10.07SGNFLCSDD86 pKa = 3.61DD87 pKa = 3.88TDD89 pKa = 3.6QIRR92 pKa = 11.84QIRR95 pKa = 11.84YY96 pKa = 8.23GDD98 pKa = 3.36LRR100 pKa = 11.84KK101 pKa = 9.42IQKK104 pKa = 10.25KK105 pKa = 9.0ILKK108 pKa = 9.93LADD111 pKa = 3.95DD112 pKa = 3.77QNDD115 pKa = 3.51RR116 pKa = 11.84QISYY120 pKa = 10.26FYY122 pKa = 11.06DD123 pKa = 4.36PDD125 pKa = 4.3GGWAGKK131 pKa = 9.59SWLTIHH137 pKa = 6.29LWEE140 pKa = 5.16RR141 pKa = 11.84GNCFVVPRR149 pKa = 11.84SKK151 pKa = 10.23ATAEE155 pKa = 4.02KK156 pKa = 10.29LSAFICSGYY165 pKa = 10.06KK166 pKa = 9.42AEE168 pKa = 4.35EE169 pKa = 4.43YY170 pKa = 10.35IVIDD174 pKa = 4.53LPRR177 pKa = 11.84APTGDD182 pKa = 2.87IGLYY186 pKa = 10.15EE187 pKa = 4.32LLEE190 pKa = 4.23DD191 pKa = 3.84TKK193 pKa = 11.46NRR195 pKa = 11.84LIFYY199 pKa = 10.34SRR201 pKa = 11.84YY202 pKa = 9.58QPITRR207 pKa = 11.84NIRR210 pKa = 11.84CPNLIVFSNHH220 pKa = 6.4KK221 pKa = 10.4LDD223 pKa = 3.82TKK225 pKa = 10.67RR226 pKa = 11.84LSADD230 pKa = 3.13RR231 pKa = 11.84CQYY234 pKa = 11.09YY235 pKa = 10.51DD236 pKa = 3.76LSVWDD241 pKa = 4.29PLEE244 pKa = 4.01VTLL247 pKa = 5.97

Molecular weight: 29.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

605

247

358

302.5

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.793 ± 0.267

1.488 ± 0.766

7.603 ± 0.741

5.785 ± 0.394

4.132 ± 0.183

7.438 ± 1.467

1.818 ± 0.347

6.612 ± 0.384

5.785 ± 0.624

9.256 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 1.043

4.628 ± 1.02

6.281 ± 1.269

3.471 ± 0.132

5.62 ± 1.409

5.124 ± 0.309

6.446 ± 0.903

4.298 ± 0.142

1.983 ± 0.253

4.793 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski