Lake Sarah-associated circular virus-21

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA94|A0A126GA94_9VIRU Coat protein OS=Lake Sarah-associated circular virus-21 OX=1685748 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 3.73VQQQIKK8 pKa = 7.28TQSIEE13 pKa = 3.89MANGITTAFWGMVINNPDD31 pKa = 3.62DD32 pKa = 3.76NDD34 pKa = 3.23FVLIHH39 pKa = 6.58NGHH42 pKa = 7.25PDD44 pKa = 2.87HH45 pKa = 6.72CRR47 pKa = 11.84EE48 pKa = 5.05LIHH51 pKa = 6.09TLEE54 pKa = 4.85KK55 pKa = 11.09GEE57 pKa = 4.62NGTIHH62 pKa = 5.63IQAWLKK68 pKa = 10.24LQRR71 pKa = 11.84QQRR74 pKa = 11.84LSFVKK79 pKa = 10.56KK80 pKa = 10.33LFPRR84 pKa = 11.84ANFKK88 pKa = 10.52PLTSDD93 pKa = 3.33EE94 pKa = 4.56YY95 pKa = 10.8IRR97 pKa = 11.84NAKK100 pKa = 10.09SYY102 pKa = 10.04AQKK105 pKa = 10.67LDD107 pKa = 3.28KK108 pKa = 10.08TAQSPSIHH116 pKa = 5.99VFQDD120 pKa = 2.91PMITLEE126 pKa = 3.98SVMRR130 pKa = 11.84KK131 pKa = 9.23LVEE134 pKa = 3.94HH135 pKa = 6.86VIQEE139 pKa = 4.32TSEE142 pKa = 3.94KK143 pKa = 10.81DD144 pKa = 3.69YY145 pKa = 11.01MDD147 pKa = 3.98EE148 pKa = 4.5RR149 pKa = 11.84CPAQCWDD156 pKa = 3.53RR157 pKa = 11.84YY158 pKa = 9.71KK159 pKa = 11.16LHH161 pKa = 7.49GDD163 pKa = 3.73TIMRR167 pKa = 11.84SMVVDD172 pKa = 4.14DD173 pKa = 4.37FRR175 pKa = 11.84IAKK178 pKa = 9.44VFVSDD183 pKa = 3.64VFKK186 pKa = 10.99KK187 pKa = 9.5IWKK190 pKa = 9.66DD191 pKa = 3.14YY192 pKa = 8.58GRR194 pKa = 11.84EE195 pKa = 4.05VFVSIHH201 pKa = 5.62NAHH204 pKa = 5.99THH206 pKa = 4.1THH208 pKa = 4.9TQRR211 pKa = 11.84EE212 pKa = 4.0DD213 pKa = 3.43DD214 pKa = 4.18EE215 pKa = 5.8NNVAVVDD222 pKa = 4.06IPTTNADD229 pKa = 3.3DD230 pKa = 4.07DD231 pKa = 4.26TRR233 pKa = 11.84SGQGSRR239 pKa = 11.84ILEE242 pKa = 4.36GEE244 pKa = 4.07HH245 pKa = 7.77DD246 pKa = 5.03DD247 pKa = 4.01GTEE250 pKa = 3.92QEE252 pKa = 4.64DD253 pKa = 3.87SEE255 pKa = 6.19DD256 pKa = 3.71YY257 pKa = 11.18DD258 pKa = 3.52EE259 pKa = 6.16CEE261 pKa = 4.45TEE263 pKa = 4.06GSEE266 pKa = 4.61GSSEE270 pKa = 4.37SGDD273 pKa = 3.72SQSDD277 pKa = 3.79EE278 pKa = 4.36EE279 pKa = 5.78DD280 pKa = 3.58DD281 pKa = 3.74

Molecular weight: 32.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA94|A0A126GA94_9VIRU Coat protein OS=Lake Sarah-associated circular virus-21 OX=1685748 PE=4 SV=1
MM1 pKa = 7.82PMTTRR6 pKa = 11.84GAGKK10 pKa = 10.22GAGFWKK16 pKa = 10.67GSTTTGRR23 pKa = 11.84SKK25 pKa = 10.03KK26 pKa = 7.09TVKK29 pKa = 8.93ITTSVRR35 pKa = 11.84PKK37 pKa = 9.93VPKK40 pKa = 10.16GAAKK44 pKa = 10.4AGIVSLMKK52 pKa = 10.82KK53 pKa = 9.36MISQGEE59 pKa = 4.17EE60 pKa = 3.47TKK62 pKa = 11.02YY63 pKa = 10.33VALNNFTPLQPVLVPAGASSPINSMLPILSQGTDD97 pKa = 2.5DD98 pKa = 3.41WQRR101 pKa = 11.84IGEE104 pKa = 4.29QIQPTAASVNFRR116 pKa = 11.84FQFIPEE122 pKa = 4.02VNATEE127 pKa = 4.47DD128 pKa = 3.79LTVVLYY134 pKa = 10.28ILTYY138 pKa = 10.45KK139 pKa = 10.08KK140 pKa = 10.82VKK142 pKa = 10.29SFPLAIADD150 pKa = 3.59PTVCQFLDD158 pKa = 3.45NGQGAAQFFQPNNEE172 pKa = 4.1ALGPPAVYY180 pKa = 10.38DD181 pKa = 3.7CGAQISAMMPIHH193 pKa = 6.98KK194 pKa = 8.76STHH197 pKa = 4.63TLLKK201 pKa = 9.93KK202 pKa = 10.21KK203 pKa = 9.59VFRR206 pKa = 11.84FVRR209 pKa = 11.84NQGVTDD215 pKa = 4.34FDD217 pKa = 4.06ATPSATPNIRR227 pKa = 11.84ANHH230 pKa = 6.22HH231 pKa = 6.07EE232 pKa = 4.01YY233 pKa = 10.0TYY235 pKa = 11.22KK236 pKa = 10.54FKK238 pKa = 10.96KK239 pKa = 10.0LPKK242 pKa = 10.02LKK244 pKa = 11.03YY245 pKa = 9.37EE246 pKa = 4.89DD247 pKa = 3.66NHH249 pKa = 6.37AQYY252 pKa = 10.14PSNFAPFALAYY263 pKa = 10.4VYY265 pKa = 10.21TNSGRR270 pKa = 11.84ALATPSPIGWSMRR283 pKa = 11.84SNLYY287 pKa = 10.61FKK289 pKa = 10.85DD290 pKa = 3.56SS291 pKa = 3.39

Molecular weight: 31.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

572

281

291

286.0

32.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.993 ± 1.759

1.049 ± 0.242

7.168 ± 2.496

5.769 ± 2.02

4.545 ± 0.637

5.769 ± 0.508

3.147 ± 0.956

5.594 ± 0.524

6.993 ± 0.839

5.594 ± 0.625

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.797 ± 0.032

4.72 ± 0.291

5.42 ± 1.662

5.42 ± 0.407

4.196 ± 0.508

6.993 ± 0.08

7.343 ± 0.605

6.294 ± 0.072

1.224 ± 0.129

2.972 ± 0.541
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski