Stappia indica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Stappiaceae; Stappia

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4539 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285SR42|A0A285SR42_9RHOB CRP/FNR family transcriptional regulator anaerobic regulatory protein OS=Stappia indica OX=538381 GN=SAMN05421512_106280 PE=4 SV=1
MM1 pKa = 8.03SEE3 pKa = 3.9EE4 pKa = 3.91HH5 pKa = 6.91RR6 pKa = 11.84EE7 pKa = 3.9PDD9 pKa = 3.58GLTEE13 pKa = 4.31PDD15 pKa = 3.1EE16 pKa = 5.5GYY18 pKa = 10.82DD19 pKa = 4.59EE20 pKa = 4.85EE21 pKa = 5.9LLDD24 pKa = 5.38LFDD27 pKa = 5.77DD28 pKa = 3.82YY29 pKa = 11.84RR30 pKa = 11.84EE31 pKa = 4.14AFEE34 pKa = 6.2DD35 pKa = 3.84YY36 pKa = 10.61DD37 pKa = 4.39AEE39 pKa = 4.3AVADD43 pKa = 4.11CFAYY47 pKa = 9.46PAVIWQFGKK56 pKa = 11.29GNVFQDD62 pKa = 3.87GEE64 pKa = 4.31EE65 pKa = 3.86LLEE68 pKa = 4.59NIEE71 pKa = 4.38KK72 pKa = 10.73LFAALEE78 pKa = 4.28KK79 pKa = 10.46EE80 pKa = 4.51GVVASAFDD88 pKa = 3.35ILSAHH93 pKa = 5.95VEE95 pKa = 3.92GDD97 pKa = 3.1AAMATLTWTQSDD109 pKa = 3.82ADD111 pKa = 3.99GEE113 pKa = 4.38AVIEE117 pKa = 4.6FTCHH121 pKa = 3.9YY122 pKa = 10.07HH123 pKa = 7.66LIYY126 pKa = 11.05DD127 pKa = 4.06GDD129 pKa = 3.38AWRR132 pKa = 11.84IAMIVNEE139 pKa = 4.21PP140 pKa = 3.33

Molecular weight: 15.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285TBQ2|A0A285TBQ2_9RHOB Cupin superfamily protein OS=Stappia indica OX=538381 GN=SAMN05421512_11045 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.48NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4539

0

4539

1479176

25

6900

325.9

35.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.128 ± 0.055

0.826 ± 0.011

5.665 ± 0.024

6.052 ± 0.039

3.664 ± 0.024

8.927 ± 0.044

1.942 ± 0.017

4.889 ± 0.027

2.716 ± 0.029

10.427 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.502 ± 0.018

2.298 ± 0.028

5.177 ± 0.031

2.87 ± 0.019

7.45 ± 0.041

5.305 ± 0.028

5.255 ± 0.04

7.574 ± 0.029

1.259 ± 0.013

2.073 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski