Frankia canadensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7042 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I2KPJ2|A0A2I2KPJ2_9ACTN PemK-like protein OS=Frankia canadensis OX=1836972 GN=FRACA_1960010 PE=4 SV=1
MM1 pKa = 7.4ATPAKK6 pKa = 9.72PGVSADD12 pKa = 3.13LRR14 pKa = 11.84VRR16 pKa = 11.84VDD18 pKa = 3.4QGACTGDD25 pKa = 3.75GLCVQLAPSVFEE37 pKa = 4.03FDD39 pKa = 3.48VDD41 pKa = 3.27GLAYY45 pKa = 10.77VKK47 pKa = 10.85DD48 pKa = 3.87EE49 pKa = 4.4TGDD52 pKa = 3.66LQTSEE57 pKa = 3.99GAYY60 pKa = 10.32VGVPLPVVNAVLDD73 pKa = 4.22AADD76 pKa = 4.43EE77 pKa = 4.51CPGTCIYY84 pKa = 8.8VTRR87 pKa = 11.84PDD89 pKa = 3.4GTIEE93 pKa = 4.11AGGPADD99 pKa = 3.38

Molecular weight: 10.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I2KL22|A0A2I2KL22_9ACTN Uncharacterized protein OS=Frankia canadensis OX=1836972 GN=FRACA_1380004 PE=4 SV=1
MM1 pKa = 7.59AKK3 pKa = 10.27GRR5 pKa = 11.84GVPAALVVRR14 pKa = 11.84RR15 pKa = 11.84VMLARR20 pKa = 11.84VTRR23 pKa = 11.84FARR26 pKa = 11.84TGRR29 pKa = 11.84AGRR32 pKa = 11.84LVRR35 pKa = 11.84AAPVRR40 pKa = 11.84ACRR43 pKa = 11.84WLWGTALIARR53 pKa = 11.84RR54 pKa = 11.84AAVAATQQ61 pKa = 3.21

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7042

0

7042

2157780

20

4564

306.4

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.636 ± 0.047

0.8 ± 0.009

6.13 ± 0.025

4.785 ± 0.025

2.529 ± 0.018

9.827 ± 0.031

2.247 ± 0.016

3.242 ± 0.02

1.24 ± 0.016

10.108 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.603 ± 0.011

1.528 ± 0.014

6.763 ± 0.033

2.504 ± 0.015

9.126 ± 0.031

5.224 ± 0.024

5.974 ± 0.03

8.548 ± 0.029

1.424 ± 0.011

1.76 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski