Mycobacterium phage MilleniumForce

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KRJ9|A0A386KRJ9_9CAUD Uncharacterized protein OS=Mycobacterium phage MilleniumForce OX=2315711 GN=1 PE=4 SV=1
MM1 pKa = 7.98PDD3 pKa = 3.43SDD5 pKa = 5.65PIDD8 pKa = 3.45FTAAGEE14 pKa = 4.07AFAEE18 pKa = 4.23IFMDD22 pKa = 4.96GIRR25 pKa = 11.84AIIAQEE31 pKa = 3.5LDD33 pKa = 3.05ARR35 pKa = 11.84GVKK38 pKa = 10.45GPSTVVNNVVPYY50 pKa = 10.34SLPDD54 pKa = 3.59SQDD57 pKa = 3.03AQYY60 pKa = 10.67MEE62 pKa = 4.28ATYY65 pKa = 10.41EE66 pKa = 4.01ADD68 pKa = 3.24VV69 pKa = 3.7

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KMS7|A0A386KMS7_9CAUD Uncharacterized protein OS=Mycobacterium phage MilleniumForce OX=2315711 GN=84 PE=4 SV=1
MM1 pKa = 7.21VARR4 pKa = 11.84PMVTIHH10 pKa = 6.57RR11 pKa = 11.84RR12 pKa = 11.84TVHH15 pKa = 5.94KK16 pKa = 10.82QFTKK20 pKa = 10.21QIAWEE25 pKa = 4.43KK26 pKa = 9.83EE27 pKa = 3.69LQAYY31 pKa = 7.44RR32 pKa = 11.84TMPWATPKK40 pKa = 10.9LIDD43 pKa = 4.8FGPMWIEE50 pKa = 3.98VEE52 pKa = 4.07RR53 pKa = 11.84CTPILNIHH61 pKa = 6.14PNWSRR66 pKa = 11.84RR67 pKa = 11.84YY68 pKa = 9.88AEE70 pKa = 4.53PLWDD74 pKa = 4.07LLAAIHH80 pKa = 6.56AAGWWHH86 pKa = 7.44CDD88 pKa = 2.83PCLINVVVHH97 pKa = 6.4PDD99 pKa = 3.27RR100 pKa = 11.84GVLLIDD106 pKa = 4.48FEE108 pKa = 4.65NLTLATGNRR117 pKa = 11.84SYY119 pKa = 11.62DD120 pKa = 3.21LYY122 pKa = 10.41GATRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84CRR130 pKa = 11.84TRR132 pKa = 11.84LARR135 pKa = 11.84TRR137 pKa = 11.84PRR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84ALEE144 pKa = 3.7RR145 pKa = 11.84TMAVVPRR152 pKa = 11.84TLLGRR157 pKa = 11.84TMIGIVQHH165 pKa = 6.43PDD167 pKa = 2.29EE168 pKa = 4.41WWRR171 pKa = 11.84HH172 pKa = 4.07KK173 pKa = 10.95

Molecular weight: 20.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

18539

30

1215

178.3

19.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.68 ± 0.478

1.316 ± 0.175

6.505 ± 0.205

5.831 ± 0.336

2.87 ± 0.213

8.652 ± 0.514

2.233 ± 0.18

4.353 ± 0.172

3.247 ± 0.172

7.374 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.12

3.35 ± 0.144

6.16 ± 0.187

3.371 ± 0.187

7.109 ± 0.384

5.755 ± 0.266

6.78 ± 0.257

7.277 ± 0.271

2.239 ± 0.158

2.568 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski