Brachyspira pilosicoli (strain ATCC BAA-1826 / 95/1000)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Brachyspirales; Brachyspiraceae; Brachyspira; Brachyspira pilosicoli

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8IBM7|D8IBM7_BRAP9 Cell division protein FtsZ OS=Brachyspira pilosicoli (strain ATCC BAA-1826 / 95/1000) OX=759914 GN=ftsZ PE=3 SV=1
MM1 pKa = 7.37FGVFMIKK8 pKa = 10.01KK9 pKa = 9.48VLLLVLFLFVACNNKK24 pKa = 10.28DD25 pKa = 3.76NLDD28 pKa = 3.75KK29 pKa = 11.38NQEE32 pKa = 3.86NTEE35 pKa = 4.2NIEE38 pKa = 3.97NNYY41 pKa = 9.84IDD43 pKa = 4.06TNQNALPEE51 pKa = 4.75TITTTTTTVKK61 pKa = 10.67SDD63 pKa = 2.81GDD65 pKa = 3.84YY66 pKa = 11.58NEE68 pKa = 4.27WFSPYY73 pKa = 10.29TFMKK77 pKa = 10.31NDD79 pKa = 3.81NGSMAQLALNDD90 pKa = 3.91MVLPEE95 pKa = 3.94YY96 pKa = 10.87NIAQFRR102 pKa = 11.84FTYY105 pKa = 9.95IDD107 pKa = 3.35EE108 pKa = 4.44NLIKK112 pKa = 9.97TYY114 pKa = 9.94FYY116 pKa = 11.69SNATIDD122 pKa = 3.65NDD124 pKa = 4.14GADD127 pKa = 4.29MYY129 pKa = 10.97AQSDD133 pKa = 3.26TGYY136 pKa = 8.86TIRR139 pKa = 11.84GNYY142 pKa = 8.02YY143 pKa = 9.55NKK145 pKa = 10.45KK146 pKa = 8.75FTITTKK152 pKa = 9.87IDD154 pKa = 3.44EE155 pKa = 4.42VDD157 pKa = 3.17ISASDD162 pKa = 4.34FISAVSYY169 pKa = 11.06NYY171 pKa = 10.65LYY173 pKa = 10.79TNSSQNDD180 pKa = 3.61SLGNGSTFFYY190 pKa = 10.12KK191 pKa = 10.64DD192 pKa = 3.61SIFVIIPKK200 pKa = 8.77DD201 pKa = 3.66TNNLEE206 pKa = 4.26VYY208 pKa = 10.62DD209 pKa = 5.78KK210 pKa = 10.48ITLDD214 pKa = 3.25INNNIDD220 pKa = 3.64NIVRR224 pKa = 11.84DD225 pKa = 3.54EE226 pKa = 4.16RR227 pKa = 11.84LWLKK231 pKa = 11.12NEE233 pKa = 3.91TLPFFEE239 pKa = 5.1KK240 pKa = 10.79AMSDD244 pKa = 4.88FYY246 pKa = 11.69NDD248 pKa = 3.04WYY250 pKa = 10.14TNSDD254 pKa = 3.3FNYY257 pKa = 10.13EE258 pKa = 4.09YY259 pKa = 10.49IAGKK263 pKa = 10.22DD264 pKa = 2.92ILYY267 pKa = 10.79FNDD270 pKa = 3.25TTISINSYY278 pKa = 9.65SSIYY282 pKa = 9.46MGGAHH287 pKa = 6.44GVYY290 pKa = 10.6NNTSLVYY297 pKa = 10.24SLKK300 pKa = 10.82DD301 pKa = 3.28GEE303 pKa = 4.98KK304 pKa = 10.13ISITNLIKK312 pKa = 10.7DD313 pKa = 4.18FKK315 pKa = 10.89DD316 pKa = 2.99EE317 pKa = 4.05KK318 pKa = 10.96LRR320 pKa = 11.84ISMKK324 pKa = 10.6SKK326 pKa = 10.4LLSISDD332 pKa = 3.75RR333 pKa = 11.84TEE335 pKa = 3.32EE336 pKa = 4.69DD337 pKa = 3.81YY338 pKa = 11.24LVPLDD343 pKa = 5.07EE344 pKa = 4.7ITLEE348 pKa = 4.05DD349 pKa = 3.25TSFYY353 pKa = 10.77VYY355 pKa = 10.66KK356 pKa = 10.85DD357 pKa = 3.59GIHH360 pKa = 5.82FVWPIYY366 pKa = 10.32TITAYY371 pKa = 10.64VQGEE375 pKa = 4.38TEE377 pKa = 3.73IVYY380 pKa = 10.7SFDD383 pKa = 3.63EE384 pKa = 3.87IRR386 pKa = 11.84PFVNEE391 pKa = 3.8EE392 pKa = 3.58YY393 pKa = 10.77LYY395 pKa = 10.5ILEE398 pKa = 4.32EE399 pKa = 4.07

Molecular weight: 46.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8ICZ5|D8ICZ5_BRAP9 30S ribosomal protein S10 OS=Brachyspira pilosicoli (strain ATCC BAA-1826 / 95/1000) OX=759914 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.48ARR3 pKa = 11.84LALKK7 pKa = 10.57VKK9 pKa = 8.9ATKK12 pKa = 8.79KK13 pKa = 9.94QKK15 pKa = 10.86YY16 pKa = 6.83KK17 pKa = 8.55TRR19 pKa = 11.84QYY21 pKa = 10.82NRR23 pKa = 11.84CPICGRR29 pKa = 11.84PRR31 pKa = 11.84AYY33 pKa = 9.34IRR35 pKa = 11.84QYY37 pKa = 10.9KK38 pKa = 7.87MCRR41 pKa = 11.84ICFRR45 pKa = 11.84DD46 pKa = 3.63LANKK50 pKa = 10.02GLIPGVTKK58 pKa = 10.8SSWW61 pKa = 2.87

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2301

0

2301

762952

34

5567

331.6

37.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.917 ± 0.059

0.88 ± 0.018

5.939 ± 0.057

6.857 ± 0.082

4.844 ± 0.047

5.482 ± 0.071

1.293 ± 0.021

10.386 ± 0.071

8.876 ± 0.053

8.765 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 0.025

7.591 ± 0.081

2.674 ± 0.031

2.014 ± 0.023

3.155 ± 0.03

6.633 ± 0.044

4.879 ± 0.041

5.768 ± 0.049

0.611 ± 0.015

4.935 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski