Firmicutes bacterium CAG:536

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5ZBT4|R5ZBT4_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:536 OX=1263028 GN=BN700_00522 PE=4 SV=1
MM1 pKa = 7.31IANYY5 pKa = 9.79DD6 pKa = 3.95VNVSSAPSKK15 pKa = 10.83DD16 pKa = 3.13ATQVDD21 pKa = 4.84YY22 pKa = 10.37IYY24 pKa = 11.15SGTTVNIIKK33 pKa = 8.86TVQNGNEE40 pKa = 3.77TWGQIGDD47 pKa = 4.24DD48 pKa = 3.13RR49 pKa = 11.84WVCINDD55 pKa = 3.47GNLTIISS62 pKa = 3.87

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5Z2E5|R5Z2E5_9FIRM Nitroreductase family protein OS=Firmicutes bacterium CAG:536 OX=1263028 GN=BN700_00385 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84EE3 pKa = 3.56KK4 pKa = 10.26IQRR7 pKa = 11.84FFYY10 pKa = 10.49GRR12 pKa = 11.84YY13 pKa = 9.74GLDD16 pKa = 3.03RR17 pKa = 11.84LNQFILVLALIIEE30 pKa = 4.57VINLFFRR37 pKa = 11.84SFVLNGIFYY46 pKa = 9.93VLILIYY52 pKa = 9.06MFRR55 pKa = 11.84ILSKK59 pKa = 10.87NIVARR64 pKa = 11.84SIEE67 pKa = 3.78NDD69 pKa = 2.64KK70 pKa = 10.72YY71 pKa = 10.97LRR73 pKa = 11.84YY74 pKa = 9.77KK75 pKa = 10.51KK76 pKa = 10.36FVIRR80 pKa = 11.84RR81 pKa = 11.84GKK83 pKa = 8.36TFQSNIKK90 pKa = 10.09DD91 pKa = 3.72RR92 pKa = 11.84EE93 pKa = 4.35HH94 pKa = 7.27KK95 pKa = 10.73VVLCPKK101 pKa = 8.32CAQMVRR107 pKa = 11.84LPRR110 pKa = 11.84KK111 pKa = 9.08RR112 pKa = 11.84GKK114 pKa = 10.97LEE116 pKa = 3.96VTCPNCHH123 pKa = 6.49AKK125 pKa = 10.33FEE127 pKa = 4.35KK128 pKa = 9.97RR129 pKa = 11.84TT130 pKa = 3.6

Molecular weight: 15.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1621

0

1621

496565

29

1557

306.3

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.141 ± 0.049

1.66 ± 0.024

5.981 ± 0.051

6.676 ± 0.062

4.351 ± 0.047

6.294 ± 0.055

2.241 ± 0.024

7.473 ± 0.055

7.375 ± 0.053

9.182 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.11 ± 0.028

4.48 ± 0.05

3.212 ± 0.029

4.239 ± 0.041

3.675 ± 0.045

5.94 ± 0.044

5.162 ± 0.041

6.904 ± 0.047

0.843 ± 0.019

4.059 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski