Gordonia phage Madeline

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nymbaxtervirinae; Nymphadoravirus; unclassified Nymphadoravirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514A328|A0A514A328_9CAUD Helix-turn-helix DNA binding domain protein OS=Gordonia phage Madeline OX=2591189 GN=72 PE=4 SV=1
MM1 pKa = 7.17STPLVPDD8 pKa = 3.18WTDD11 pKa = 2.91SDD13 pKa = 4.06IEE15 pKa = 4.34IEE17 pKa = 4.33VPDD20 pKa = 4.07NQVQVIDD27 pKa = 4.08APAVEE32 pKa = 4.43DD33 pKa = 3.94VEE35 pKa = 4.62VLVIQGRR42 pKa = 11.84DD43 pKa = 3.71GQGLQPDD50 pKa = 4.17AAVDD54 pKa = 4.21TYY56 pKa = 12.04ADD58 pKa = 4.33LPDD61 pKa = 4.88DD62 pKa = 5.43LGPNDD67 pKa = 4.05SGQVVYY73 pKa = 10.71VWSDD77 pKa = 2.91KK78 pKa = 11.07LIYY81 pKa = 9.62IWSGTDD87 pKa = 3.02WPAEE91 pKa = 3.95GAGKK95 pKa = 9.87DD96 pKa = 3.4IRR98 pKa = 11.84GMQGDD103 pKa = 4.39PGRR106 pKa = 11.84GIDD109 pKa = 3.62DD110 pKa = 3.73VFVVGTSLVFSMSDD124 pKa = 3.14NTSDD128 pKa = 4.28DD129 pKa = 3.82VPVPAIQQAIDD140 pKa = 3.28SAAAASGSATAADD153 pKa = 4.08TARR156 pKa = 11.84LAAEE160 pKa = 4.66AAASTAGTAASTATTEE176 pKa = 3.77RR177 pKa = 11.84TAAQTARR184 pKa = 11.84TAAEE188 pKa = 4.14AARR191 pKa = 11.84DD192 pKa = 3.65TATNRR197 pKa = 11.84ATAASNSADD206 pKa = 3.11AAATSEE212 pKa = 4.26ANAEE216 pKa = 4.11TSEE219 pKa = 4.54DD220 pKa = 3.61NAATSAAAAATSAGQAANRR239 pKa = 11.84ATDD242 pKa = 3.31ADD244 pKa = 4.03TARR247 pKa = 11.84AAAVLARR254 pKa = 11.84DD255 pKa = 3.72AAAGSATDD263 pKa = 3.64AATSAGSAQTSADD276 pKa = 3.71DD277 pKa = 4.46AGTSAAAAAASAEE290 pKa = 4.09EE291 pKa = 4.16AADD294 pKa = 3.74VVAAGVPDD302 pKa = 3.56ATVTTKK308 pKa = 10.93GGVRR312 pKa = 11.84LAGDD316 pKa = 4.57LGGTWDD322 pKa = 4.35EE323 pKa = 4.25PTVPALAMKK332 pKa = 10.57ADD334 pKa = 4.18LDD336 pKa = 4.64SNGKK340 pKa = 9.07LLSSQMPAQATHH352 pKa = 6.33EE353 pKa = 4.64SVVVTSTAEE362 pKa = 3.9RR363 pKa = 11.84LALTPAQVQPGDD375 pKa = 3.47TAIQLGNPGRR385 pKa = 11.84GTYY388 pKa = 10.03SLQGADD394 pKa = 3.84PSDD397 pKa = 3.83PGSWVLQVAPTDD409 pKa = 3.58AVSSVNGYY417 pKa = 9.61QGIVVLGKK425 pKa = 10.87GDD427 pKa = 3.53VGLGNVDD434 pKa = 3.46NTSDD438 pKa = 3.61LAKK441 pKa = 10.07PISTAAQTALDD452 pKa = 3.98GKK454 pKa = 10.39VDD456 pKa = 3.87EE457 pKa = 4.75VSTANVAYY465 pKa = 7.43GTKK468 pKa = 9.61TGGVQGTWPVTSAATATTLALRR490 pKa = 11.84GTGGTVAVGTATGPTHH506 pKa = 7.37APTKK510 pKa = 9.82QQLDD514 pKa = 3.72DD515 pKa = 4.24GLSGKK520 pKa = 10.37SDD522 pKa = 3.2TGHH525 pKa = 6.61AHH527 pKa = 6.98DD528 pKa = 5.03AAAIATGTLDD538 pKa = 3.78AARR541 pKa = 11.84LPVGTGSTQVAAGNDD556 pKa = 3.49SRR558 pKa = 11.84IVNAVPNSRR567 pKa = 11.84TVTAGTGLSGGGTLDD582 pKa = 3.23VNRR585 pKa = 11.84TLSVLYY591 pKa = 10.92GNTANTATQGNDD603 pKa = 2.58ARR605 pKa = 11.84LSDD608 pKa = 3.77TRR610 pKa = 11.84TPTDD614 pKa = 3.41NTVSTAKK621 pKa = 10.2IQDD624 pKa = 3.75GAVTLAKK631 pKa = 10.32LATAVSVSIQQMIDD645 pKa = 3.08ASVLAAQLVTINAQTGAYY663 pKa = 8.53TLVATDD669 pKa = 3.4ANKK672 pKa = 10.46AVEE675 pKa = 4.54VTSATAVNVTIPTDD689 pKa = 3.34AVNFPIGTVIEE700 pKa = 4.21IDD702 pKa = 3.17QMGAGKK708 pKa = 10.56VSIVGASGVTVKK720 pKa = 10.36PDD722 pKa = 3.11SPTPTTRR729 pKa = 11.84TQYY732 pKa = 10.75SALVLRR738 pKa = 11.84KK739 pKa = 9.31RR740 pKa = 11.84AANWWLVTGDD750 pKa = 3.84LAA752 pKa = 6.13

Molecular weight: 74.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514A2W6|A0A514A2W6_9CAUD Membrane protein OS=Gordonia phage Madeline OX=2591189 GN=23 PE=4 SV=1
MM1 pKa = 7.91RR2 pKa = 11.84APLTRR7 pKa = 11.84SRR9 pKa = 11.84RR10 pKa = 11.84SMADD14 pKa = 2.86PLARR18 pKa = 11.84FFTQPFTVRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 8.31TGDD32 pKa = 3.38GAVGPIYY39 pKa = 10.44ADD41 pKa = 3.29PVTLRR46 pKa = 11.84GRR48 pKa = 11.84VNATNRR54 pKa = 11.84LVIDD58 pKa = 3.7DD59 pKa = 4.91RR60 pKa = 11.84GNQVLSAAKK69 pKa = 9.23ISMSITEE76 pKa = 4.56DD77 pKa = 4.38DD78 pKa = 4.19IPTGSQARR86 pKa = 11.84VGVGPWRR93 pKa = 11.84TVIADD98 pKa = 3.51SRR100 pKa = 11.84HH101 pKa = 4.94VGGFHH106 pKa = 7.62KK107 pKa = 11.05SPDD110 pKa = 3.72YY111 pKa = 11.29YY112 pKa = 11.39SIDD115 pKa = 3.74LNN117 pKa = 3.96

Molecular weight: 12.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

16941

47

2146

225.9

24.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.859 ± 0.676

0.697 ± 0.15

7.066 ± 0.375

5.637 ± 0.285

2.574 ± 0.194

8.317 ± 0.344

2.007 ± 0.206

4.48 ± 0.16

3.288 ± 0.197

7.745 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.178

2.904 ± 0.206

5.82 ± 0.254

3.601 ± 0.255

7.739 ± 0.45

5.661 ± 0.216

7.278 ± 0.343

7.131 ± 0.227

1.913 ± 0.152

2.166 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski