Vibrio phage V05

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Schizotequatrovirus; unclassified Schizotequatrovirus

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H0X7F8|A0A6H0X7F8_9CAUD Uncharacterized protein OS=Vibrio phage V05 OX=2724325 GN=OLCHANIL_00238 PE=4 SV=1
MM1 pKa = 6.97THH3 pKa = 7.42DD4 pKa = 4.94LNMLLEE10 pKa = 4.29QYY12 pKa = 11.18NLANYY17 pKa = 10.24DD18 pKa = 3.51CDD20 pKa = 5.2GDD22 pKa = 3.99FEE24 pKa = 5.38DD25 pKa = 5.76YY26 pKa = 10.03YY27 pKa = 10.59WSVAEE32 pKa = 4.74WIGQTVFSHH41 pKa = 5.2GTIRR45 pKa = 11.84KK46 pKa = 9.07HH47 pKa = 7.39DD48 pKa = 4.01ILEE51 pKa = 4.23LLCDD55 pKa = 3.98LLEE58 pKa = 5.06ISEE61 pKa = 4.29DD62 pKa = 3.66QINVDD67 pKa = 4.83DD68 pKa = 4.75VDD70 pKa = 4.73DD71 pKa = 4.76FLTEE75 pKa = 3.86VYY77 pKa = 10.24EE78 pKa = 4.36VIEE81 pKa = 4.1

Molecular weight: 9.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H0X785|A0A6H0X785_9CAUD Tail tube terminator protein OS=Vibrio phage V05 OX=2724325 GN=OLCHANIL_00179 PE=4 SV=1
MM1 pKa = 7.56ARR3 pKa = 11.84PEE5 pKa = 3.75RR6 pKa = 11.84AKK8 pKa = 10.56KK9 pKa = 10.3KK10 pKa = 10.47KK11 pKa = 10.06EE12 pKa = 3.85SLLDD16 pKa = 3.41SFKK19 pKa = 11.33AQTHH23 pKa = 5.04EE24 pKa = 4.65LGRR27 pKa = 11.84TPIAKK32 pKa = 9.5NQRR35 pKa = 11.84KK36 pKa = 8.85SLAWFKK42 pKa = 11.19KK43 pKa = 9.13RR44 pKa = 11.84VHH46 pKa = 6.28DD47 pKa = 5.28AIRR50 pKa = 11.84SRR52 pKa = 11.84KK53 pKa = 8.44VQVPHH58 pKa = 6.94KK59 pKa = 10.2GNMYY63 pKa = 10.07VFAYY67 pKa = 9.81DD68 pKa = 4.3AKK70 pKa = 9.94TKK72 pKa = 10.26HH73 pKa = 5.78QLPYY77 pKa = 9.26WDD79 pKa = 5.07KK80 pKa = 10.21FPCIICLGQQKK91 pKa = 10.96GYY93 pKa = 9.2MLGLNLHH100 pKa = 6.75YY101 pKa = 10.26IPPKK105 pKa = 10.34DD106 pKa = 3.44RR107 pKa = 11.84EE108 pKa = 4.27KK109 pKa = 11.04FLTTLLRR116 pKa = 11.84YY117 pKa = 10.06ANTKK121 pKa = 8.31TVSNSTRR128 pKa = 11.84LMIDD132 pKa = 3.25WGRR135 pKa = 11.84VKK137 pKa = 10.58NIKK140 pKa = 9.62FSKK143 pKa = 10.51HH144 pKa = 3.75MVKK147 pKa = 10.48LYY149 pKa = 9.08ILKK152 pKa = 10.17RR153 pKa = 11.84IKK155 pKa = 10.73GSLEE159 pKa = 3.56EE160 pKa = 4.33VKK162 pKa = 10.64PHH164 pKa = 6.31DD165 pKa = 4.54WYY167 pKa = 11.71NVIHH171 pKa = 6.55MPLQQFVAKK180 pKa = 10.06NGRR183 pKa = 11.84NISAVRR189 pKa = 11.84AYY191 pKa = 10.48DD192 pKa = 3.18DD193 pKa = 3.49RR194 pKa = 11.84YY195 pKa = 10.65RR196 pKa = 11.84RR197 pKa = 11.84RR198 pKa = 3.37

Molecular weight: 23.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

237

0

237

46555

37

1165

196.4

22.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.168 ± 0.193

1.145 ± 0.08

6.609 ± 0.14

7.348 ± 0.23

4.275 ± 0.121

6.12 ± 0.202

2.234 ± 0.091

6.487 ± 0.142

6.279 ± 0.27

7.527 ± 0.136

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.775 ± 0.111

5.338 ± 0.137

3.243 ± 0.108

3.49 ± 0.097

4.809 ± 0.1

6.218 ± 0.196

6.266 ± 0.279

6.837 ± 0.12

1.486 ± 0.069

4.345 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski