Sedimentisphaera salicampi

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Phycisphaerae; Sedimentisphaerales; Sedimentisphaeraceae; Sedimentisphaera

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2335 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6LJS1|A0A1W6LJS1_9BACT Efflux pump membrane transporter BepG OS=Sedimentisphaera salicampi OX=1941349 GN=bepG PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.45RR3 pKa = 11.84LISLMLGIVLSLSLCSFGDD22 pKa = 4.08YY23 pKa = 9.29ITIDD27 pKa = 3.41SDD29 pKa = 3.72LSDD32 pKa = 3.36WAGIDD37 pKa = 3.6YY38 pKa = 9.89TDD40 pKa = 3.87QGTKK44 pKa = 10.09SGPDD48 pKa = 3.1GGSYY52 pKa = 10.03SLRR55 pKa = 11.84TTYY58 pKa = 10.89DD59 pKa = 3.13SQNLYY64 pKa = 10.15IAVDD68 pKa = 3.79RR69 pKa = 11.84SSTGRR74 pKa = 11.84ILGDD78 pKa = 2.95TGTNYY83 pKa = 11.03DD84 pKa = 3.96DD85 pKa = 3.99TSYY88 pKa = 11.66SPDD91 pKa = 3.34DD92 pKa = 3.76SFFLAFDD99 pKa = 3.35VDD101 pKa = 4.9GITDD105 pKa = 3.49SGGSSNVTLANGEE118 pKa = 4.08TDD120 pKa = 3.03GMSISFGGDD129 pKa = 3.1NLPDD133 pKa = 4.19IIYY136 pKa = 10.35AFSGGGNWFTYY147 pKa = 7.54STWNGSEE154 pKa = 3.78WTGDD158 pKa = 2.94ADD160 pKa = 3.49NWVEE164 pKa = 4.64PEE166 pKa = 4.15DD167 pKa = 4.04SSIGVSYY174 pKa = 10.91GDD176 pKa = 3.91SGNDD180 pKa = 3.09GDD182 pKa = 5.07EE183 pKa = 4.19FAIPLQDD190 pKa = 3.11IGSPSGDD197 pKa = 2.59IMAWAWMAKK206 pKa = 8.89EE207 pKa = 3.49SHH209 pKa = 6.51YY210 pKa = 10.83YY211 pKa = 9.62IEE213 pKa = 4.57AAWGFSSGAQPTAYY227 pKa = 10.19DD228 pKa = 3.85GMSVAVPEE236 pKa = 4.44PFTLGLLGIGSIALRR251 pKa = 11.84RR252 pKa = 11.84RR253 pKa = 11.84RR254 pKa = 11.84KK255 pKa = 9.57SS256 pKa = 3.07

Molecular weight: 27.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6LJ69|A0A1W6LJ69_9BACT Ig-like domain-containing protein OS=Sedimentisphaera salicampi OX=1941349 GN=STSP1_00204 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.23NQRR5 pKa = 11.84RR6 pKa = 11.84NKK8 pKa = 9.39KK9 pKa = 9.71RR10 pKa = 11.84RR11 pKa = 11.84TATIRR16 pKa = 11.84EE17 pKa = 3.97QNQCRR22 pKa = 11.84FCRR25 pKa = 11.84AKK27 pKa = 10.01TEE29 pKa = 4.12YY30 pKa = 10.33VDD32 pKa = 4.82YY33 pKa = 10.81MDD35 pKa = 5.74IDD37 pKa = 4.07TLSKK41 pKa = 10.99LLTNRR46 pKa = 11.84GKK48 pKa = 9.86IYY50 pKa = 10.11SRR52 pKa = 11.84KK53 pKa = 9.59RR54 pKa = 11.84SGNCASCQRR63 pKa = 11.84KK64 pKa = 7.67VQNAIKK70 pKa = 9.77RR71 pKa = 11.84ARR73 pKa = 11.84YY74 pKa = 8.34MGLLPYY80 pKa = 8.91QTT82 pKa = 4.47

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2335

0

2335

937580

34

3411

401.5

44.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.652 ± 0.062

1.403 ± 0.019

6.024 ± 0.047

7.405 ± 0.046

4.44 ± 0.035

7.645 ± 0.055

1.644 ± 0.022

6.536 ± 0.038

6.58 ± 0.065

8.453 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.023

4.693 ± 0.033

4.055 ± 0.03

3.268 ± 0.025

4.612 ± 0.042

7.26 ± 0.045

4.769 ± 0.042

6.156 ± 0.036

1.404 ± 0.025

3.658 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski