Bemisia-associated genomovirus NfO

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus bemta1

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z2X293|A0A1Z2X293_9VIRU Spliced replication-associated protein OS=Bemisia-associated genomovirus NfO OX=1986479 GN=Rep PE=3 SV=2
MM1 pKa = 8.01PFRR4 pKa = 11.84FQAKK8 pKa = 9.39YY9 pKa = 11.04GLLTYY14 pKa = 7.2PQCGDD19 pKa = 3.73LDD21 pKa = 3.59PWHH24 pKa = 6.69IVDD27 pKa = 4.21MLGDD31 pKa = 4.37LGAEE35 pKa = 4.22CIIGRR40 pKa = 11.84EE41 pKa = 4.03DD42 pKa = 3.19HH43 pKa = 6.47VAGGVHH49 pKa = 5.93LHH51 pKa = 6.28AFFMFEE57 pKa = 4.26RR58 pKa = 11.84KK59 pKa = 9.22FEE61 pKa = 4.24SRR63 pKa = 11.84NVRR66 pKa = 11.84VFDD69 pKa = 4.3VDD71 pKa = 3.32GHH73 pKa = 6.37HH74 pKa = 7.12PNVVPGYY81 pKa = 7.34STPEE85 pKa = 4.02KK86 pKa = 9.74GWTYY90 pKa = 9.57ATKK93 pKa = 10.78DD94 pKa = 3.22GDD96 pKa = 3.74IVAGGLEE103 pKa = 4.36CPRR106 pKa = 11.84MRR108 pKa = 11.84EE109 pKa = 4.13EE110 pKa = 4.1VSGASEE116 pKa = 3.64KK117 pKa = 9.97WSRR120 pKa = 11.84ILLSEE125 pKa = 4.03SRR127 pKa = 11.84DD128 pKa = 3.58EE129 pKa = 4.44FFEE132 pKa = 4.41TVARR136 pKa = 11.84LDD138 pKa = 3.64PRR140 pKa = 11.84ALCINFGSLRR150 pKa = 11.84AYY152 pKa = 10.41ADD154 pKa = 2.68WRR156 pKa = 11.84YY157 pKa = 10.15RR158 pKa = 11.84PTRR161 pKa = 11.84DD162 pKa = 3.17PYY164 pKa = 9.54VTPEE168 pKa = 4.01GLSFDD173 pKa = 3.45TSGFPEE179 pKa = 4.54LDD181 pKa = 2.82QWVRR185 pKa = 11.84EE186 pKa = 4.29SLVGLPGTRR195 pKa = 11.84PRR197 pKa = 11.84SLIIIGDD204 pKa = 3.64TRR206 pKa = 11.84LGKK209 pKa = 7.79TLWARR214 pKa = 11.84SLGKK218 pKa = 9.39HH219 pKa = 5.95AYY221 pKa = 9.9FGGLFCLDD229 pKa = 3.79EE230 pKa = 4.48SLEE233 pKa = 4.3DD234 pKa = 2.93VDD236 pKa = 5.06YY237 pKa = 11.57AVFDD241 pKa = 4.16DD242 pKa = 4.05MQGGLEE248 pKa = 4.23FFHH251 pKa = 7.36SYY253 pKa = 10.65KK254 pKa = 10.45FWLGAQKK261 pKa = 10.36QFYY264 pKa = 8.95ATDD267 pKa = 3.37KK268 pKa = 10.83YY269 pKa = 10.66RR270 pKa = 11.84GKK272 pKa = 10.87KK273 pKa = 9.35LVDD276 pKa = 2.98WGRR279 pKa = 11.84PSIYY283 pKa = 9.9ISNTNPLADD292 pKa = 4.19KK293 pKa = 10.9GADD296 pKa = 3.53VDD298 pKa = 4.06WLMGNCTIVHH308 pKa = 6.27VDD310 pKa = 3.28TAIFRR315 pKa = 11.84ANTEE319 pKa = 3.84

Molecular weight: 36.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z2X293|A0A1Z2X293_9VIRU Spliced replication-associated protein OS=Bemisia-associated genomovirus NfO OX=1986479 GN=Rep PE=3 SV=2
MM1 pKa = 6.64TTLFFPVKK9 pKa = 9.57STARR13 pKa = 11.84PLKK16 pKa = 8.73MYY18 pKa = 10.28RR19 pKa = 11.84RR20 pKa = 11.84STRR23 pKa = 11.84RR24 pKa = 11.84SAGPRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84TTRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84YY37 pKa = 8.32PVKK40 pKa = 10.28KK41 pKa = 9.4RR42 pKa = 11.84FSRR45 pKa = 11.84RR46 pKa = 11.84TTPTKK51 pKa = 10.13RR52 pKa = 11.84IMRR55 pKa = 11.84RR56 pKa = 11.84TSKK59 pKa = 10.48RR60 pKa = 11.84RR61 pKa = 11.84ILTITSKK68 pKa = 10.71KK69 pKa = 10.27KK70 pKa = 9.73RR71 pKa = 11.84DD72 pKa = 3.48TMLVLTNVQDD82 pKa = 3.49NRR84 pKa = 11.84AITTSYY90 pKa = 10.54NSNGAVMNATAWNGAPWRR108 pKa = 11.84MLWCATARR116 pKa = 11.84NNQNGTGAVGLPDD129 pKa = 3.52NQATRR134 pKa = 11.84TAQACYY140 pKa = 9.71MRR142 pKa = 11.84GLKK145 pKa = 9.55EE146 pKa = 3.79QIEE149 pKa = 5.09FIMNSSLPWQWRR161 pKa = 11.84RR162 pKa = 11.84IVFRR166 pKa = 11.84TKK168 pKa = 10.46GIQAILSEE176 pKa = 4.38TSTSYY181 pKa = 10.87FSNLTSGGYY190 pKa = 10.35ARR192 pKa = 11.84TVNEE196 pKa = 4.57ANTATATNLEE206 pKa = 4.11EE207 pKa = 4.21VLFKK211 pKa = 11.0GKK213 pKa = 9.97QGSDD217 pKa = 2.76WNDD220 pKa = 2.41IFIASTDD227 pKa = 3.41NSRR230 pKa = 11.84VTTMYY235 pKa = 11.2DD236 pKa = 2.86RR237 pKa = 11.84TRR239 pKa = 11.84NLNAGAEE246 pKa = 4.3GGRR249 pKa = 11.84IQKK252 pKa = 9.97VSVWHH257 pKa = 6.27PMNKK261 pKa = 8.23TLVYY265 pKa = 10.05XDD267 pKa = 5.46DD268 pKa = 3.78EE269 pKa = 4.83TGGTEE274 pKa = 3.84ATALYY279 pKa = 9.64SNPSIGLGDD288 pKa = 3.37VWVYY292 pKa = 11.68DD293 pKa = 4.15LFRR296 pKa = 11.84PAYY299 pKa = 8.71GGSGSLVFKK308 pKa = 10.28PQATLYY314 pKa = 8.68WHH316 pKa = 6.98EE317 pKa = 4.28KK318 pKa = 9.3

Molecular weight: 36.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

637

318

319

318.5

36.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.064 ± 0.575

1.256 ± 0.452

5.965 ± 1.808

4.553 ± 1.015

4.71 ± 1.129

8.32 ± 1.011

1.727 ± 0.792

4.082 ± 0.005

4.71 ± 0.459

7.535 ± 0.899

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.456

4.396 ± 1.593

4.396 ± 0.449

2.512 ± 0.456

8.948 ± 1.258

6.279 ± 0.688

8.477 ± 2.504

5.651 ± 0.447

2.826 ± 0.003

3.925 ± 0.109
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski