Klebsiella phage KP179

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Jiaodavirus; unclassified Jiaodavirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 260 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386K8P0|A0A386K8P0_9CAUD Ribonucleoside-diphosphate reductase OS=Klebsiella phage KP179 OX=2315700 PE=3 SV=1
MM1 pKa = 7.18AQVTVEE7 pKa = 3.88IYY9 pKa = 10.25DD10 pKa = 3.63YY11 pKa = 10.95EE12 pKa = 4.5HH13 pKa = 7.11FIEE16 pKa = 4.86TIEE19 pKa = 4.16KK20 pKa = 9.25YY21 pKa = 10.99GLIEE25 pKa = 4.11VSNKK29 pKa = 8.69SAPWGGNEE37 pKa = 3.68ITVEE41 pKa = 4.02GDD43 pKa = 3.78TPTLWLWLEE52 pKa = 3.9QEE54 pKa = 4.78YY55 pKa = 10.26FPGMDD60 pKa = 3.67DD61 pKa = 3.43EE62 pKa = 4.82CRR64 pKa = 11.84EE65 pKa = 4.14DD66 pKa = 3.49TLTTFSGG73 pKa = 3.48

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386K6P6|A0A386K6P6_9CAUD Uncharacterized protein OS=Klebsiella phage KP179 OX=2315700 PE=4 SV=1
MM1 pKa = 7.91AKK3 pKa = 10.1RR4 pKa = 11.84ISKK7 pKa = 10.04RR8 pKa = 11.84RR9 pKa = 11.84LKK11 pKa = 10.3IIRR14 pKa = 11.84KK15 pKa = 7.02QKK17 pKa = 9.83EE18 pKa = 3.73RR19 pKa = 11.84ALVLALRR26 pKa = 11.84EE27 pKa = 4.21EE28 pKa = 4.21ITRR31 pKa = 11.84EE32 pKa = 3.35IDD34 pKa = 3.75KK35 pKa = 10.86EE36 pKa = 3.9ILKK39 pKa = 10.73ALTAAII45 pKa = 4.34

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

260

0

260

50306

38

1390

193.5

21.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.19 ± 0.181

1.087 ± 0.077

6.454 ± 0.127

7.11 ± 0.196

4.206 ± 0.118

6.403 ± 0.245

1.847 ± 0.086

6.88 ± 0.151

7.462 ± 0.202

7.293 ± 0.147

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.894 ± 0.093

5.395 ± 0.152

3.485 ± 0.106

3.361 ± 0.11

4.53 ± 0.099

6.196 ± 0.12

5.836 ± 0.238

6.777 ± 0.132

1.547 ± 0.067

4.045 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski