Dinoroseobacter phage DFL12phi1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Rhodovirinae; Baltimorevirus; Dinoroseobacter virus DFL12

Average proteome isoelectric point is 5.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023NHB8|A0A023NHB8_9CAUD Uncharacterized protein OS=Dinoroseobacter phage DFL12phi1 OX=1477404 GN=DFL12P1_0055 PE=4 SV=1
MM1 pKa = 7.74AEE3 pKa = 4.0RR4 pKa = 11.84RR5 pKa = 11.84PIVLVNGEE13 pKa = 4.21LQEE16 pKa = 4.57LPSGDD21 pKa = 3.96TLPGSGGASAFTALSDD37 pKa = 3.72TPADD41 pKa = 3.69FTGQALKK48 pKa = 10.64AIRR51 pKa = 11.84VNSGEE56 pKa = 4.16TALEE60 pKa = 4.08FTDD63 pKa = 4.62FPSGGSGGQLTAMFIDD79 pKa = 4.42AQPSVTGWSTAVSATNAATKK99 pKa = 10.05GNRR102 pKa = 11.84VRR104 pKa = 11.84VQYY107 pKa = 10.8DD108 pKa = 2.96IDD110 pKa = 4.26LKK112 pKa = 10.47TVTFQGNNTSSQTYY126 pKa = 7.79EE127 pKa = 4.14CVVAEE132 pKa = 4.48ISGTTIGTIVGRR144 pKa = 11.84VTQVSTGGADD154 pKa = 3.34EE155 pKa = 5.84FITFTFGTALRR166 pKa = 11.84LVPAQDD172 pKa = 3.52YY173 pKa = 11.02AILITQQGGTGSTQLEE189 pKa = 4.38SHH191 pKa = 6.61AGDD194 pKa = 3.32GGMTGVLSSYY204 pKa = 11.28DD205 pKa = 3.45LGGYY209 pKa = 9.91IIDD212 pKa = 4.26NDD214 pKa = 3.67IQGSEE219 pKa = 4.15TFVAFGSSYY228 pKa = 11.51VGMEE232 pKa = 3.81CTYY235 pKa = 11.01DD236 pKa = 3.3IVLTVNGVGSGILSGTVDD254 pKa = 3.72PTTEE258 pKa = 4.13GNDD261 pKa = 2.87GDD263 pKa = 3.95FYY265 pKa = 11.64YY266 pKa = 9.83RR267 pKa = 11.84TDD269 pKa = 3.06TNTIFGPKK277 pKa = 9.51NGTWPAGVSLVGPEE291 pKa = 4.44GPVTTMVQTEE301 pKa = 4.21ITATAYY307 pKa = 9.13STVSADD313 pKa = 3.24FAGNVVRR320 pKa = 11.84RR321 pKa = 11.84MNNAAAQTITVEE333 pKa = 4.15PSMTGGQPVTFIATGAGAVSFAAGVGVTIHH363 pKa = 6.31SAGGNLTIADD373 pKa = 3.9QYY375 pKa = 11.89GSATLIPDD383 pKa = 3.62ATTANTYY390 pKa = 10.22YY391 pKa = 11.08LIGNLTTT398 pKa = 4.3

Molecular weight: 40.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023NHX2|A0A023NHX2_9CAUD Uncharacterized protein OS=Dinoroseobacter phage DFL12phi1 OX=1477404 GN=DFL12P1_0084 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.3SEE4 pKa = 4.5RR5 pKa = 11.84FPPLVSQEE13 pKa = 4.26EE14 pKa = 4.19LDD16 pKa = 4.07ADD18 pKa = 3.68ASTTLFSRR26 pKa = 11.84IIRR29 pKa = 11.84MTQMLRR35 pKa = 11.84NSRR38 pKa = 11.84WSFWRR43 pKa = 3.54

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

23750

34

3555

276.2

30.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.198 ± 0.411

0.699 ± 0.141

6.636 ± 0.172

7.427 ± 0.295

3.802 ± 0.188

7.2 ± 0.28

1.794 ± 0.187

5.634 ± 0.17

5.528 ± 0.359

7.899 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.251 ± 0.143

5.204 ± 0.177

4.261 ± 0.195

4.598 ± 0.19

4.661 ± 0.231

5.752 ± 0.162

6.442 ± 0.259

6.383 ± 0.198

1.389 ± 0.139

3.242 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski