Staphylococcus phage SpT99F3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Peeveelvirus; unclassified Peeveelvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0MFL0|A0A1J0MFL0_9CAUD Uncharacterized protein OS=Staphylococcus phage SpT99F3 OX=1913449 GN=SpT99F3_010 PE=4 SV=1
MM1 pKa = 7.4MIKK4 pKa = 9.74IYY6 pKa = 10.8KK7 pKa = 9.61NEE9 pKa = 3.86NDD11 pKa = 3.52EE12 pKa = 4.82LEE14 pKa = 4.32CHH16 pKa = 6.0VNYY19 pKa = 10.56AGYY22 pKa = 10.33DD23 pKa = 3.51FKK25 pKa = 11.29FQCIKK30 pKa = 10.95DD31 pKa = 3.46NYY33 pKa = 9.09GAIFEE38 pKa = 4.86GSNSTEE44 pKa = 3.53YY45 pKa = 10.96RR46 pKa = 11.84EE47 pKa = 3.96FEE49 pKa = 4.4SYY51 pKa = 10.79IDD53 pKa = 3.4VDD55 pKa = 4.22NEE57 pKa = 4.01VLRR60 pKa = 11.84NLQDD64 pKa = 3.05VMYY67 pKa = 10.21NIASVYY73 pKa = 8.73NWRR76 pKa = 11.84EE77 pKa = 3.79SFGEE81 pKa = 4.02EE82 pKa = 3.58RR83 pKa = 4.2

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0MFZ5|A0A1J0MFZ5_9CAUD Uncharacterized protein OS=Staphylococcus phage SpT99F3 OX=1913449 GN=SpT99F3_036 PE=4 SV=1
MM1 pKa = 7.44ILSNSVRR8 pKa = 11.84QRR10 pKa = 11.84YY11 pKa = 7.38RR12 pKa = 11.84TDD14 pKa = 3.24TVGKK18 pKa = 8.39TPTEE22 pKa = 3.9LQKK25 pKa = 10.55EE26 pKa = 4.12LRR28 pKa = 11.84KK29 pKa = 10.13RR30 pKa = 11.84GVRR33 pKa = 11.84GFVVNVSHH41 pKa = 6.88NRR43 pKa = 11.84VTMLVDD49 pKa = 3.23RR50 pKa = 11.84RR51 pKa = 11.84DD52 pKa = 3.25VKK54 pKa = 9.98TNKK57 pKa = 9.82EE58 pKa = 3.97CMRR61 pKa = 11.84CLTSTEE67 pKa = 3.88TRR69 pKa = 11.84KK70 pKa = 10.33

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12317

31

1480

181.1

20.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.456 ± 0.489

0.544 ± 0.121

6.479 ± 0.392

7.258 ± 0.582

4.043 ± 0.186

5.488 ± 0.668

1.624 ± 0.163

7.153 ± 0.277

8.947 ± 0.292

7.956 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.622 ± 0.226

6.69 ± 0.263

2.557 ± 0.144

4.003 ± 0.256

4.668 ± 0.255

6.292 ± 0.4

6.195 ± 0.263

6.414 ± 0.253

1.153 ± 0.15

4.457 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski