Streptococcus phage IPP62

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SF55|A0A1S5SF55_9CAUD Uncharacterized protein OS=Streptococcus phage IPP62 OX=1916198 GN=IPP62_00025 PE=4 SV=1
MM1 pKa = 7.56SLTLNSTIGDD11 pKa = 3.66LVLAIGEE18 pKa = 4.54IIVGSDD24 pKa = 2.82GKK26 pKa = 7.93TTTAILEE33 pKa = 4.24IPDD36 pKa = 3.18QSFYY40 pKa = 11.68LEE42 pKa = 4.42IEE44 pKa = 4.3LKK46 pKa = 10.85LKK48 pKa = 10.96EE49 pKa = 4.03EE50 pKa = 4.68VINN53 pKa = 4.05

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SF44|A0A1S5SF44_9CAUD DNA replication protein OS=Streptococcus phage IPP62 OX=1916198 GN=IPP62_00016 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.59LIGVRR7 pKa = 11.84FLGITLLFSRR17 pKa = 11.84SFLYY21 pKa = 10.69VLILINLFINVKK33 pKa = 10.13KK34 pKa = 10.18FANFLDD40 pKa = 3.84NFLKK44 pKa = 10.66GGEE47 pKa = 4.05EE48 pKa = 3.9MRR50 pKa = 11.84PRR52 pKa = 11.84RR53 pKa = 11.84YY54 pKa = 9.4PYY56 pKa = 10.05SGKK59 pKa = 10.14KK60 pKa = 9.95EE61 pKa = 4.14STLVKK66 pKa = 10.31ADD68 pKa = 3.68PEE70 pKa = 4.2LVEE73 pKa = 4.85KK74 pKa = 10.51ILRR77 pKa = 11.84NTSFLEE83 pKa = 3.85RR84 pKa = 11.84LQVLLATKK92 pKa = 9.83PP93 pKa = 3.33

Molecular weight: 10.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12681

30

3302

226.4

25.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.145 ± 0.687

0.576 ± 0.132

5.851 ± 0.37

8.044 ± 0.374

4.306 ± 0.428

6.537 ± 0.311

1.396 ± 0.223

6.372 ± 0.302

8.201 ± 0.357

8.73 ± 0.434

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.346

5.733 ± 0.291

2.468 ± 0.2

4.006 ± 0.362

4.905 ± 0.297

6.506 ± 0.709

5.97 ± 0.527

6.048 ± 0.267

1.27 ± 0.113

3.564 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski