Roseococcus sp. SYP-B2431

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseococcus; unclassified Roseococcus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4416 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R0YEK1|A0A4R0YEK1_9PROT Uncharacterized protein OS=Roseococcus sp. SYP-B2431 OX=2496640 GN=EJV46_10485 PE=4 SV=1
YYY2 pKa = 9.47EE3 pKa = 4.47FSGSGTTIIAAEEE16 pKa = 3.86SGRR19 pKa = 11.84SCHHH23 pKa = 6.36VEEE26 pKa = 5.31SPAYYY31 pKa = 10.58DDD33 pKa = 3.58AVLRR37 pKa = 11.84WEEE40 pKa = 4.42FTGCLAVLEEE50 pKa = 4.64DDD52 pKa = 3.85ASYYY56 pKa = 10.78ATAALRR62 pKa = 11.84AEEE65 pKa = 4.53EE66 pKa = 4.36ATGAGEEE73 pKa = 4.12T

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R0YHB5|A0A4R0YHB5_9PROT Disulfide bond formation protein B OS=Roseococcus sp. SYP-B2431 OX=2496640 GN=EJV46_06325 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.54GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 9.11VLANRR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.87GRR39 pKa = 11.84AKK41 pKa = 10.73LSAA44 pKa = 3.92

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4416

0

4416

1429663

26

2400

323.7

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.435 ± 0.069

0.835 ± 0.01

4.863 ± 0.022

5.735 ± 0.036

3.398 ± 0.024

9.332 ± 0.037

2.029 ± 0.018

4.302 ± 0.025

2.107 ± 0.033

10.818 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.527 ± 0.016

2.13 ± 0.02

6.227 ± 0.034

3.001 ± 0.021

8.364 ± 0.041

4.665 ± 0.03

4.875 ± 0.025

7.149 ± 0.029

1.467 ± 0.017

1.741 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski