Lactococcus phage P087

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Teubervirus; Lactococcus virus P087

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C3U2L6|C3U2L6_9CAUD Uncharacterized protein OS=Lactococcus phage P087 OX=641487 PE=4 SV=1
MM1 pKa = 7.84IDD3 pKa = 3.35RR4 pKa = 11.84LEE6 pKa = 4.96DD7 pKa = 3.34YY8 pKa = 9.55TVAAIAFMKK17 pKa = 10.77AGDD20 pKa = 3.78QWSSFYY26 pKa = 11.34DD27 pKa = 3.39KK28 pKa = 11.32DD29 pKa = 3.42GVYY32 pKa = 10.15YY33 pKa = 10.12PSSNTVDD40 pKa = 3.94FAVAHH45 pKa = 6.54SIAA48 pKa = 3.91

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C3U2N8|C3U2N8_9CAUD Uncharacterized protein OS=Lactococcus phage P087 OX=641487 PE=4 SV=1
MM1 pKa = 7.21KK2 pKa = 9.97RR3 pKa = 11.84FYY5 pKa = 10.71RR6 pKa = 11.84IFVKK10 pKa = 10.91NEE12 pKa = 3.34IIFRR16 pKa = 11.84IFTRR20 pKa = 11.84NIRR23 pKa = 11.84RR24 pKa = 11.84ILTQNKK30 pKa = 8.36PVNHH34 pKa = 7.11ACLTAKK40 pKa = 10.27IFWGNFLGIQCEE52 pKa = 4.1II53 pKa = 4.08

Molecular weight: 6.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

18361

29

1309

208.6

23.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.051 ± 0.426

1.116 ± 0.174

6.47 ± 0.196

6.819 ± 0.442

4.058 ± 0.191

6.154 ± 0.46

1.59 ± 0.143

6.868 ± 0.218

7.037 ± 0.277

8.687 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.794 ± 0.123

6.291 ± 0.232

3.284 ± 0.185

3.916 ± 0.203

3.6 ± 0.205

6.307 ± 0.311

7.031 ± 0.285

6.579 ± 0.238

1.138 ± 0.1

4.21 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski