Lachnospiraceae bacterium 3_1_57FAA_CT1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7KHC9|F7KHC9_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium 3_1_57FAA_CT1 OX=658086 GN=HMPREF0994_05268 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.16KK3 pKa = 10.17KK4 pKa = 10.68VIAALLTSAMAVSLLAGCGTTVEE27 pKa = 4.69STGSSQSTSTTEE39 pKa = 4.25TKK41 pKa = 8.79EE42 pKa = 3.76TAAPAEE48 pKa = 4.36TEE50 pKa = 4.07TAAVEE55 pKa = 4.34TEE57 pKa = 4.12VAEE60 pKa = 4.52EE61 pKa = 4.45DD62 pKa = 3.88NTDD65 pKa = 3.23LSANITLWTYY75 pKa = 10.65PIGKK79 pKa = 8.33WGDD82 pKa = 3.57SATVDD87 pKa = 4.12SLITSFNAKK96 pKa = 9.57YY97 pKa = 10.28PNINVTVEE105 pKa = 3.79YY106 pKa = 10.84LDD108 pKa = 3.71YY109 pKa = 11.43TNGDD113 pKa = 3.76DD114 pKa = 4.58QVNTAIEE121 pKa = 4.66GGQAPDD127 pKa = 3.74LVMEE131 pKa = 4.7GPEE134 pKa = 3.55RR135 pKa = 11.84LVANWGAKK143 pKa = 9.82GLMVDD148 pKa = 5.07LSDD151 pKa = 5.11IMDD154 pKa = 5.31DD155 pKa = 3.66EE156 pKa = 4.83DD157 pKa = 3.65QSEE160 pKa = 4.74IYY162 pKa = 10.77GSVLAACMTKK172 pKa = 10.21EE173 pKa = 3.98GAVYY177 pKa = 9.21EE178 pKa = 4.46YY179 pKa = 8.93PLCMTAHH186 pKa = 6.68CMAINKK192 pKa = 7.92TVFEE196 pKa = 4.53AAGAMQYY203 pKa = 10.67VDD205 pKa = 4.64EE206 pKa = 6.05DD207 pKa = 3.95SHH209 pKa = 5.84TWTTEE214 pKa = 3.81DD215 pKa = 3.34FFKK218 pKa = 11.22AIDD221 pKa = 3.4AVYY224 pKa = 9.92AHH226 pKa = 6.79TGSTVGAVYY235 pKa = 10.61CSGQGGDD242 pKa = 2.96QGTRR246 pKa = 11.84ALINNLYY253 pKa = 10.69GGTFTDD259 pKa = 4.97DD260 pKa = 3.12LHH262 pKa = 6.33TKK264 pKa = 8.31YY265 pKa = 9.04TADD268 pKa = 3.2SAEE271 pKa = 4.08NVKK274 pKa = 10.48AIQALADD281 pKa = 3.72TDD283 pKa = 3.84GKK285 pKa = 10.69IAFDD289 pKa = 3.65ASIAGGDD296 pKa = 3.84EE297 pKa = 3.95INLFRR302 pKa = 11.84QGVLNVAFCWNIAQQLNSDD321 pKa = 4.14NNDD324 pKa = 3.2AGLTNNGDD332 pKa = 4.11EE333 pKa = 4.43IVFMAFPAEE342 pKa = 4.26KK343 pKa = 10.39ADD345 pKa = 3.43ATQLCGGIWGFGVFDD360 pKa = 4.48NGDD363 pKa = 3.6ADD365 pKa = 5.19RR366 pKa = 11.84IAASKK371 pKa = 10.81LFIKK375 pKa = 10.72YY376 pKa = 8.53MADD379 pKa = 3.59SADD382 pKa = 3.51GTADD386 pKa = 3.39AVLSSTYY393 pKa = 10.12FPVRR397 pKa = 11.84DD398 pKa = 3.77TVEE401 pKa = 4.31GTDD404 pKa = 4.0LTGLYY409 pKa = 10.38NDD411 pKa = 3.66VPTMQEE417 pKa = 3.91YY418 pKa = 10.77SKK420 pKa = 11.23LMQFLGDD427 pKa = 4.01YY428 pKa = 10.0YY429 pKa = 10.98QVTPGWTEE437 pKa = 4.11ARR439 pKa = 11.84TAWWNMLQQVGSGADD454 pKa = 3.16VQTAVDD460 pKa = 4.15EE461 pKa = 4.88FVSTANAAAAAQQ473 pKa = 3.52

Molecular weight: 50.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7KH83|F7KH83_9FIRM ABC transmembrane type-1 domain-containing protein OS=Lachnospiraceae bacterium 3_1_57FAA_CT1 OX=658086 GN=HMPREF0994_05222 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.64NRR10 pKa = 11.84QRR12 pKa = 11.84NKK14 pKa = 8.02VHH16 pKa = 6.59GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6505

0

6505

2265984

29

3054

348.3

39.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.42 ± 0.029

1.595 ± 0.014

5.468 ± 0.024

7.745 ± 0.036

4.448 ± 0.023

7.298 ± 0.025

1.686 ± 0.014

6.902 ± 0.025

6.055 ± 0.024

9.472 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.119 ± 0.016

4.16 ± 0.019

3.614 ± 0.017

3.28 ± 0.017

4.809 ± 0.025

5.903 ± 0.023

5.115 ± 0.023

6.37 ± 0.023

1.188 ± 0.013

4.353 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski